Abstract
The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
| Original language | English |
|---|---|
| Pages (from-to) | 799-801 |
| Number of pages | 3 |
| Journal | Journal of biochemistry |
| Volume | 107 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1990 Jun |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
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Itaya, M., Yamaguchi, I., Kobayashi, K., Endo, T., & Tanaka, T. (1990). The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. Journal of biochemistry, 107(6), 799-801. https://doi.org/10.1093/oxfordjournals.jbchem.a123128