The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome

Mitsuhiro Itaya, Isamu Yamaguchi, Kaori Kobayashi, Toyoshige Endo, Teruo Tanaka

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.

Original languageEnglish
Pages (from-to)799-801
Number of pages3
JournalJournal of biochemistry
Volume107
Issue number6
DOIs
Publication statusPublished - 1990 Jun

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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