The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome

Mitsuhiro Itaya, Isamu Yamaguchi, Kaori Kobayashi, Toyoshige Endo, Teruo Tanaka

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43 Citations (Scopus)

Abstract

The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.

Original languageEnglish
Pages (from-to)799-801
Number of pages3
JournalJournal of Biochemistry
Volume107
Issue number6
Publication statusPublished - 1990 Jun
Externally publishedYes

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ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Itaya, M., Yamaguchi, I., Kobayashi, K., Endo, T., & Tanaka, T. (1990). The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. Journal of Biochemistry, 107(6), 799-801.