TY - JOUR
T1 - The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome
AU - Itaya, Mitsuhiro
AU - Yamaguchi, Isamu
AU - Kobayashi, Kaori
AU - Endo, Toyoshige
AU - Tanaka, Teruo
PY - 1990/6
Y1 - 1990/6
N2 - The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
AB - The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
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U2 - 10.1093/oxfordjournals.jbchem.a123128
DO - 10.1093/oxfordjournals.jbchem.a123128
M3 - Article
C2 - 2118136
AN - SCOPUS:0025290798
VL - 107
SP - 799
EP - 801
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 6
ER -