Abstract
The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
| Original language | English |
|---|---|
| Pages (from-to) | 799-801 |
| Number of pages | 3 |
| Journal | Journal of Biochemistry |
| Volume | 107 |
| Issue number | 6 |
| Publication status | Published - 1990 Jun |
| Externally published | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Physiology (medical)
- Radiology Nuclear Medicine and imaging
- Molecular Biology
- Biochemistry
Cite this
The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. / Itaya, Mitsuhiro; Yamaguchi, Isamu; Kobayashi, Kaori; Endo, Toyoshige; Tanaka, Teruo.
In: Journal of Biochemistry, Vol. 107, No. 6, 06.1990, p. 799-801.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome
AU - Itaya, Mitsuhiro
AU - Yamaguchi, Isamu
AU - Kobayashi, Kaori
AU - Endo, Toyoshige
AU - Tanaka, Teruo
PY - 1990/6
Y1 - 1990/6
N2 - The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
AB - The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025290798&partnerID=8YFLogxK
M3 - Article
C2 - 2118136
AN - SCOPUS:0025290798
VL - 107
SP - 799
EP - 801
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 6
ER -