The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome

Mitsuhiro Itaya, Isamu Yamaguchi, Kaori Kobayashi, Toyoshige Endo, Teruo Tanaka

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.

Original languageEnglish
Pages (from-to)799-801
Number of pages3
JournalJournal of Biochemistry
Volume107
Issue number6
Publication statusPublished - 1990 Jun
Externally publishedYes

Fingerprint

Bacilli
Chromosomes
Bacillus subtilis
Chromosome
Genes
Gene
Bacillus cereus
5' Flanking Region
Fragment
Resistance

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Itaya, M., Yamaguchi, I., Kobayashi, K., Endo, T., & Tanaka, T. (1990). The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. Journal of Biochemistry, 107(6), 799-801.

The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. / Itaya, Mitsuhiro; Yamaguchi, Isamu; Kobayashi, Kaori; Endo, Toyoshige; Tanaka, Teruo.

In: Journal of Biochemistry, Vol. 107, No. 6, 06.1990, p. 799-801.

Research output: Contribution to journalArticle

Itaya, M, Yamaguchi, I, Kobayashi, K, Endo, T & Tanaka, T 1990, 'The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome', Journal of Biochemistry, vol. 107, no. 6, pp. 799-801.
Itaya, Mitsuhiro ; Yamaguchi, Isamu ; Kobayashi, Kaori ; Endo, Toyoshige ; Tanaka, Teruo. / The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. In: Journal of Biochemistry. 1990 ; Vol. 107, No. 6. pp. 799-801.
@article{452a4d3baaf64e4794be721e97ee5b43,
title = "The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome",
abstract = "The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.",
author = "Mitsuhiro Itaya and Isamu Yamaguchi and Kaori Kobayashi and Toyoshige Endo and Teruo Tanaka",
year = "1990",
month = "6",
language = "English",
volume = "107",
pages = "799--801",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - The blasticidin s resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome

AU - Itaya, Mitsuhiro

AU - Yamaguchi, Isamu

AU - Kobayashi, Kaori

AU - Endo, Toyoshige

AU - Tanaka, Teruo

PY - 1990/6

Y1 - 1990/6

N2 - The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.

AB - The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.

UR - http://www.scopus.com/inward/record.url?scp=0025290798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025290798&partnerID=8YFLogxK

M3 - Article

C2 - 2118136

AN - SCOPUS:0025290798

VL - 107

SP - 799

EP - 801

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 6

ER -