The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination

Kanehiro Hayashi, Asako Furuya, Yuriko Sakamaki, Takumi Akagi, Yo Shinoda, Tetsushi Sadakata, Tsutomu Hashikawa, Kazuki Shimizu, Haruka Minami, Yoshitake Sano, Manabu Nakayama, Teiichi Furuichi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Very-KIND/Kndc1/KIAA1768 (v-KIND) is a brain-specific Ras guanine nucleotide exchange factor carrying two sets of the kinase non-catalytic C-lobe domain (KIND), and is predominantly expressed in cerebellar granule cells. Here, we report the impact of v-KIND deficiency on dendritic and synaptic growth in cerebellar granule cells in v-KIND knockout (KO) mice. Furthermore, we evaluate motor function in these animals. The gross anatomy of the cerebellum, including the cerebellar lobules, layered cerebellar cortex and densely-packed granule cell layer, in KO mice appeared normal, and was similar to wild-type (WT) mice. However, KO mice displayed an overgrowth of cerebellar granule cell dendrites, compared with WT mice, resulting in an increased number of dendrites, dendritic branches and terminals. Immunoreactivity for vGluT2 (a marker for excitatory presynapses of mossy fiber terminals) was increased in the cerebellar glomeruli of KO mice, compared with WT mice. The postsynaptic density around the terminals of mossy fibers was also increased in KO mice. Although there were no significant differences in locomotor ability between KO and WT animals in their home cages or in the open field, young adult KO mice had an increased grip strength and a tendency to exhibit better motor performance in balance-related tests compared with WT animals. Taken together, our results suggest that v-KIND is required for compact dendritic growth and proper excitatory synaptic connections in cerebellar granule cells, which are necessary for normal motor coordination and balance.

Original languageEnglish
Article numbere0173175
JournalPLoS One
Volume12
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

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Knockout Mice
granules
Brain
Animals
brain
ras Guanine Nucleotide Exchange Factors
mice
Growth
Wild Animals
Fibers
cells
Dendrites
Phosphotransferases
dendrites
Cells
Post-Synaptic Density
Cerebellar Cortex
Hand Strength
Cerebellum
Young Adult

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination. / Hayashi, Kanehiro; Furuya, Asako; Sakamaki, Yuriko; Akagi, Takumi; Shinoda, Yo; Sadakata, Tetsushi; Hashikawa, Tsutomu; Shimizu, Kazuki; Minami, Haruka; Sano, Yoshitake; Nakayama, Manabu; Furuichi, Teiichi.

In: PLoS One, Vol. 12, No. 3, e0173175, 01.03.2017.

Research output: Contribution to journalArticle

Hayashi, K, Furuya, A, Sakamaki, Y, Akagi, T, Shinoda, Y, Sadakata, T, Hashikawa, T, Shimizu, K, Minami, H, Sano, Y, Nakayama, M & Furuichi, T 2017, 'The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination', PLoS One, vol. 12, no. 3, e0173175. https://doi.org/10.1371/journal.pone.0173175
Hayashi, Kanehiro ; Furuya, Asako ; Sakamaki, Yuriko ; Akagi, Takumi ; Shinoda, Yo ; Sadakata, Tetsushi ; Hashikawa, Tsutomu ; Shimizu, Kazuki ; Minami, Haruka ; Sano, Yoshitake ; Nakayama, Manabu ; Furuichi, Teiichi. / The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination. In: PLoS One. 2017 ; Vol. 12, No. 3.
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