The cell cycle of the pseudostratified ventricular epithelium of the embryonic murine cerebral wall

Takao Takahashi, R. S. Nowakowski, V. S. Caviness

Research output: Contribution to journalArticle

497 Citations (Scopus)

Abstract

Neurons destined for the cerebral neocortex are formed in the pseudostratified ventricular epithelium (PVE) lining the ventricular cavity of the developing cerebral wall. The present study, based upon cumulative S- phase labeling with bromodeoxyuridine, is an analysis of cell cycle parameters of the PVE. It is undertaken in the dorsomedial cerebral wall of mouse embryos from the eleventh to the seventeenth gestational day (E11-E17, day of conception = E0) corresponding to the complete period of neuronogenesis. The growth fraction (fraction of sells in the population which is proliferating) is virtually 1.0 from E11 through E16. The length of the cell cycle increases from 8.1 to 18.4 hr, which corresponds to a sequence of 11 integer cell cycles over the course of neuronal cytogenesis in mice. The increase in the length of the cell cycle is due essentially to a fourfold increase in the length of G1 phase which is the only phase of the cell cycle which varies systematically. Thus, the G1 phase is most likely to be the phase of the celt cycle which is modulated by extrinsically and intrinsically acting mechanisms involved in the regulation of neuronal cytogenesis.

Original languageEnglish
Pages (from-to)6046-6057
Number of pages12
JournalJournal of Neuroscience
Volume15
Issue number9
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Cell Cycle
Epithelium
G1 Phase
Neocortex
Bromodeoxyuridine
S Phase
Embryonic Structures
Neurons
Growth
Population

Keywords

  • cell cycle
  • cerebrum
  • mouse
  • neocortex
  • neuronogenesis
  • proliferation
  • ventricular zone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The cell cycle of the pseudostratified ventricular epithelium of the embryonic murine cerebral wall. / Takahashi, Takao; Nowakowski, R. S.; Caviness, V. S.

In: Journal of Neuroscience, Vol. 15, No. 9, 1995, p. 6046-6057.

Research output: Contribution to journalArticle

@article{a41e818df53143658dfd7cdae68948d7,
title = "The cell cycle of the pseudostratified ventricular epithelium of the embryonic murine cerebral wall",
abstract = "Neurons destined for the cerebral neocortex are formed in the pseudostratified ventricular epithelium (PVE) lining the ventricular cavity of the developing cerebral wall. The present study, based upon cumulative S- phase labeling with bromodeoxyuridine, is an analysis of cell cycle parameters of the PVE. It is undertaken in the dorsomedial cerebral wall of mouse embryos from the eleventh to the seventeenth gestational day (E11-E17, day of conception = E0) corresponding to the complete period of neuronogenesis. The growth fraction (fraction of sells in the population which is proliferating) is virtually 1.0 from E11 through E16. The length of the cell cycle increases from 8.1 to 18.4 hr, which corresponds to a sequence of 11 integer cell cycles over the course of neuronal cytogenesis in mice. The increase in the length of the cell cycle is due essentially to a fourfold increase in the length of G1 phase which is the only phase of the cell cycle which varies systematically. Thus, the G1 phase is most likely to be the phase of the celt cycle which is modulated by extrinsically and intrinsically acting mechanisms involved in the regulation of neuronal cytogenesis.",
keywords = "cell cycle, cerebrum, mouse, neocortex, neuronogenesis, proliferation, ventricular zone",
author = "Takao Takahashi and Nowakowski, {R. S.} and Caviness, {V. S.}",
year = "1995",
language = "English",
volume = "15",
pages = "6046--6057",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "9",

}

TY - JOUR

T1 - The cell cycle of the pseudostratified ventricular epithelium of the embryonic murine cerebral wall

AU - Takahashi, Takao

AU - Nowakowski, R. S.

AU - Caviness, V. S.

PY - 1995

Y1 - 1995

N2 - Neurons destined for the cerebral neocortex are formed in the pseudostratified ventricular epithelium (PVE) lining the ventricular cavity of the developing cerebral wall. The present study, based upon cumulative S- phase labeling with bromodeoxyuridine, is an analysis of cell cycle parameters of the PVE. It is undertaken in the dorsomedial cerebral wall of mouse embryos from the eleventh to the seventeenth gestational day (E11-E17, day of conception = E0) corresponding to the complete period of neuronogenesis. The growth fraction (fraction of sells in the population which is proliferating) is virtually 1.0 from E11 through E16. The length of the cell cycle increases from 8.1 to 18.4 hr, which corresponds to a sequence of 11 integer cell cycles over the course of neuronal cytogenesis in mice. The increase in the length of the cell cycle is due essentially to a fourfold increase in the length of G1 phase which is the only phase of the cell cycle which varies systematically. Thus, the G1 phase is most likely to be the phase of the celt cycle which is modulated by extrinsically and intrinsically acting mechanisms involved in the regulation of neuronal cytogenesis.

AB - Neurons destined for the cerebral neocortex are formed in the pseudostratified ventricular epithelium (PVE) lining the ventricular cavity of the developing cerebral wall. The present study, based upon cumulative S- phase labeling with bromodeoxyuridine, is an analysis of cell cycle parameters of the PVE. It is undertaken in the dorsomedial cerebral wall of mouse embryos from the eleventh to the seventeenth gestational day (E11-E17, day of conception = E0) corresponding to the complete period of neuronogenesis. The growth fraction (fraction of sells in the population which is proliferating) is virtually 1.0 from E11 through E16. The length of the cell cycle increases from 8.1 to 18.4 hr, which corresponds to a sequence of 11 integer cell cycles over the course of neuronal cytogenesis in mice. The increase in the length of the cell cycle is due essentially to a fourfold increase in the length of G1 phase which is the only phase of the cell cycle which varies systematically. Thus, the G1 phase is most likely to be the phase of the celt cycle which is modulated by extrinsically and intrinsically acting mechanisms involved in the regulation of neuronal cytogenesis.

KW - cell cycle

KW - cerebrum

KW - mouse

KW - neocortex

KW - neuronogenesis

KW - proliferation

KW - ventricular zone

UR - http://www.scopus.com/inward/record.url?scp=0029026103&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029026103&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 6046

EP - 6057

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 9

ER -