TY - JOUR
T1 - The chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymerization
AU - Jaroensanti-Tanaka, Naiyanate
AU - Miyazaki, Sho
AU - Hosoi, Akito
AU - Tanaka, Keisuke
AU - Ito, Shinsaku
AU - Iuchi, Satoshi
AU - Nakano, Takeshi
AU - Kobayashi, Masatomo
AU - Nakajima, Masatoshi
AU - Asami, Tadao
N1 - Funding Information:
This work was supported by the MEXT-Supported Program for the Strategic Research Foundation at Private Universities [S1311017];KAKENHI [15H04492];KAKENHI [18H02142]; KAKENHI [26440132];
Funding Information:
This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI [grant no. 15H04492 and 18H02142 (to MN)], [grant no. 26440132 (to TA)]; by Wada Kunko-kai research foundation (to MN) and by MEXT-Supported Program for the Strategic Research Foundation at Private Universities, 2013–2017 (S1311017) from the Ministry of Education, Culture, Sports, Science and Technology of Japan for RNA sequencing experiment.
Publisher Copyright:
© 2018 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2018
Y1 - 2018
N2 - We previously found a chemical, designated as NJ15, which inhibited both auxin and brassinosteroid responses in dark-grown Arabidopsis. To study its mode of action, we performed a phenotypic screening of NJ15-low-sensitive lines among mutant pools of Arabidopsis. One line (f127) showed clear NJ15-low-sensitivity in terms of hypocotyl elongation and shoot gravitropism. After further testing, it was determined that DCR, an enzyme involved in cutin polymerization, had lost its function in the mutant, which caused its low sensitivity to NJ15. Fatty acids are the base materials for polymers such as cutin and cuticular wax. We confirmed that NJ15 affects fatty acid biosynthesis, and that it does differently from cafenstrole, a known inhibitor of cuticular wax formation. Based on these results, we propose that the target of NJ15 is likely located within the cutin polymer formation pathway.
AB - We previously found a chemical, designated as NJ15, which inhibited both auxin and brassinosteroid responses in dark-grown Arabidopsis. To study its mode of action, we performed a phenotypic screening of NJ15-low-sensitive lines among mutant pools of Arabidopsis. One line (f127) showed clear NJ15-low-sensitivity in terms of hypocotyl elongation and shoot gravitropism. After further testing, it was determined that DCR, an enzyme involved in cutin polymerization, had lost its function in the mutant, which caused its low sensitivity to NJ15. Fatty acids are the base materials for polymers such as cutin and cuticular wax. We confirmed that NJ15 affects fatty acid biosynthesis, and that it does differently from cafenstrole, a known inhibitor of cuticular wax formation. Based on these results, we propose that the target of NJ15 is likely located within the cutin polymer formation pathway.
KW - Crosstalk
KW - Cuticle
KW - DCR
KW - Fatty acid
KW - Wax
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U2 - 10.1080/09168451.2018.1484278
DO - 10.1080/09168451.2018.1484278
M3 - Article
C2 - 29912637
AN - SCOPUS:85054140105
VL - 82
SP - 1770
EP - 1779
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 10
ER -