The chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymerization

Naiyanate Jaroensanti-Tanaka, Sho Miyazaki, Akito Hosoi, Keisuke Tanaka, Shinsaku Ito, Satoshi Iuchi, Takeshi Nakano, Masatomo Kobayashi, Masatoshi Nakajima, Tadao Asami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We previously found a chemical, designated as NJ15, which inhibited both auxin and brassinosteroid responses in dark-grown Arabidopsis. To study its mode of action, we performed a phenotypic screening of NJ15-low-sensitive lines among mutant pools of Arabidopsis. One line (f127) showed clear NJ15-low-sensitivity in terms of hypocotyl elongation and shoot gravitropism. After further testing, it was determined that DCR, an enzyme involved in cutin polymerization, had lost its function in the mutant, which caused its low sensitivity to NJ15. Fatty acids are the base materials for polymers such as cutin and cuticular wax. We confirmed that NJ15 affects fatty acid biosynthesis, and that it does differently from cafenstrole, a known inhibitor of cuticular wax formation. Based on these results, we propose that the target of NJ15 is likely located within the cutin polymer formation pathway.

Original languageEnglish
Pages (from-to)1770-1779
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number10
DOIs
Publication statusPublished - 2018 Jan 1
Externally publishedYes

Fingerprint

Gravitropism
Hypocotyl
Polymerization
Elongation
UCON 50-HB-5100
Waxes
Arabidopsis
Polymers
Fatty Acids
Brassinosteroids
Indoleacetic Acids
Biosynthesis
Screening
Testing
Enzymes
cutin

Keywords

  • Crosstalk
  • Cuticle
  • DCR
  • Fatty acid
  • Wax

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

The chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymerization. / Jaroensanti-Tanaka, Naiyanate; Miyazaki, Sho; Hosoi, Akito; Tanaka, Keisuke; Ito, Shinsaku; Iuchi, Satoshi; Nakano, Takeshi; Kobayashi, Masatomo; Nakajima, Masatoshi; Asami, Tadao.

In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 10, 01.01.2018, p. 1770-1779.

Research output: Contribution to journalArticle

Jaroensanti-Tanaka, N, Miyazaki, S, Hosoi, A, Tanaka, K, Ito, S, Iuchi, S, Nakano, T, Kobayashi, M, Nakajima, M & Asami, T 2018, 'The chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymerization', Bioscience, Biotechnology and Biochemistry, vol. 82, no. 10, pp. 1770-1779. https://doi.org/10.1080/09168451.2018.1484278
Jaroensanti-Tanaka, Naiyanate ; Miyazaki, Sho ; Hosoi, Akito ; Tanaka, Keisuke ; Ito, Shinsaku ; Iuchi, Satoshi ; Nakano, Takeshi ; Kobayashi, Masatomo ; Nakajima, Masatoshi ; Asami, Tadao. / The chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymerization. In: Bioscience, Biotechnology and Biochemistry. 2018 ; Vol. 82, No. 10. pp. 1770-1779.
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