Abstract
We previously found a chemical, designated as NJ15, which inhibited both auxin and brassinosteroid responses in dark-grown Arabidopsis. To study its mode of action, we performed a phenotypic screening of NJ15-low-sensitive lines among mutant pools of Arabidopsis. One line (f127) showed clear NJ15-low-sensitivity in terms of hypocotyl elongation and shoot gravitropism. After further testing, it was determined that DCR, an enzyme involved in cutin polymerization, had lost its function in the mutant, which caused its low sensitivity to NJ15. Fatty acids are the base materials for polymers such as cutin and cuticular wax. We confirmed that NJ15 affects fatty acid biosynthesis, and that it does differently from cafenstrole, a known inhibitor of cuticular wax formation. Based on these results, we propose that the target of NJ15 is likely located within the cutin polymer formation pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 1770-1779 |
| Number of pages | 10 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 82 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2018 Jan 1 |
| Externally published | Yes |
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Keywords
- Crosstalk
- Cuticle
- DCR
- Fatty acid
- Wax
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
The chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymerization. / Jaroensanti-Tanaka, Naiyanate; Miyazaki, Sho; Hosoi, Akito; Tanaka, Keisuke; Ito, Shinsaku; Iuchi, Satoshi; Nakano, Takeshi; Kobayashi, Masatomo; Nakajima, Masatoshi; Asami, Tadao.
In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 10, 01.01.2018, p. 1770-1779.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymerization
AU - Jaroensanti-Tanaka, Naiyanate
AU - Miyazaki, Sho
AU - Hosoi, Akito
AU - Tanaka, Keisuke
AU - Ito, Shinsaku
AU - Iuchi, Satoshi
AU - Nakano, Takeshi
AU - Kobayashi, Masatomo
AU - Nakajima, Masatoshi
AU - Asami, Tadao
PY - 2018/1/1
Y1 - 2018/1/1
N2 - We previously found a chemical, designated as NJ15, which inhibited both auxin and brassinosteroid responses in dark-grown Arabidopsis. To study its mode of action, we performed a phenotypic screening of NJ15-low-sensitive lines among mutant pools of Arabidopsis. One line (f127) showed clear NJ15-low-sensitivity in terms of hypocotyl elongation and shoot gravitropism. After further testing, it was determined that DCR, an enzyme involved in cutin polymerization, had lost its function in the mutant, which caused its low sensitivity to NJ15. Fatty acids are the base materials for polymers such as cutin and cuticular wax. We confirmed that NJ15 affects fatty acid biosynthesis, and that it does differently from cafenstrole, a known inhibitor of cuticular wax formation. Based on these results, we propose that the target of NJ15 is likely located within the cutin polymer formation pathway.
AB - We previously found a chemical, designated as NJ15, which inhibited both auxin and brassinosteroid responses in dark-grown Arabidopsis. To study its mode of action, we performed a phenotypic screening of NJ15-low-sensitive lines among mutant pools of Arabidopsis. One line (f127) showed clear NJ15-low-sensitivity in terms of hypocotyl elongation and shoot gravitropism. After further testing, it was determined that DCR, an enzyme involved in cutin polymerization, had lost its function in the mutant, which caused its low sensitivity to NJ15. Fatty acids are the base materials for polymers such as cutin and cuticular wax. We confirmed that NJ15 affects fatty acid biosynthesis, and that it does differently from cafenstrole, a known inhibitor of cuticular wax formation. Based on these results, we propose that the target of NJ15 is likely located within the cutin polymer formation pathway.
KW - Crosstalk
KW - Cuticle
KW - DCR
KW - Fatty acid
KW - Wax
UR - http://www.scopus.com/inward/record.url?scp=85054140105&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054140105&partnerID=8YFLogxK
U2 - 10.1080/09168451.2018.1484278
DO - 10.1080/09168451.2018.1484278
M3 - Article
C2 - 29912637
AN - SCOPUS:85054140105
VL - 82
SP - 1770
EP - 1779
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 10
ER -