The cortical subventricular zone-specific molecule Svet1 is part of the nuclear RNA coded by the putative Netrin receptor gene Unc5d and is expressed in multipolar migrating cells

Shinji Sasaki, Hidenori Tabata, Kashiko Tachikawa, Kazunori Nakajima

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Although Svet1 RNA is a widely used marker for the subventricular zone (SVZ) of the embryonic cerebral cortex, its function remains completely unknown. We report finding that Svet1 contains a high proportion of repetitive sequences and maps in the first intron of the putative Netrin receptor gene Unc5d. The direction of transcription of Svet1 is the same as that of Unc5d. The Svet1 RNA was detected in the nucleus but not in the cytoplasm. Both Svet1/Unc5d RNAs and UNC5D protein were localized in the multipolar cells in the SVZ throughout cortical development. These results suggest that Svet1 RNA is part of the sequence of the primary transcript of Unc5d in the nucleus that is spliced out before the mRNA is transported to the cytoplasm. Thus, the previously reported "SVZ-specific expression of the Svet1 RNA" in fact indicates putative involvement of UNC5D signaling in the multipolar migrating cells.

Original languageEnglish
Pages (from-to)474-483
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume38
Issue number4
DOIs
Publication statusPublished - 2008 Aug 4

Fingerprint

Nuclear RNA
Lateral Ventricles
RNA
Genes
Cytoplasm
Nucleic Acid Repetitive Sequences
Cerebral Cortex
Introns
netrin receptors
Messenger RNA
Proteins

Keywords

  • Cerebral cortex
  • Multipolar migration
  • Subventricular zone
  • Svet1
  • Unc5d

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

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title = "The cortical subventricular zone-specific molecule Svet1 is part of the nuclear RNA coded by the putative Netrin receptor gene Unc5d and is expressed in multipolar migrating cells",
abstract = "Although Svet1 RNA is a widely used marker for the subventricular zone (SVZ) of the embryonic cerebral cortex, its function remains completely unknown. We report finding that Svet1 contains a high proportion of repetitive sequences and maps in the first intron of the putative Netrin receptor gene Unc5d. The direction of transcription of Svet1 is the same as that of Unc5d. The Svet1 RNA was detected in the nucleus but not in the cytoplasm. Both Svet1/Unc5d RNAs and UNC5D protein were localized in the multipolar cells in the SVZ throughout cortical development. These results suggest that Svet1 RNA is part of the sequence of the primary transcript of Unc5d in the nucleus that is spliced out before the mRNA is transported to the cytoplasm. Thus, the previously reported {"}SVZ-specific expression of the Svet1 RNA{"} in fact indicates putative involvement of UNC5D signaling in the multipolar migrating cells.",
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author = "Shinji Sasaki and Hidenori Tabata and Kashiko Tachikawa and Kazunori Nakajima",
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AU - Sasaki, Shinji

AU - Tabata, Hidenori

AU - Tachikawa, Kashiko

AU - Nakajima, Kazunori

PY - 2008/8/4

Y1 - 2008/8/4

N2 - Although Svet1 RNA is a widely used marker for the subventricular zone (SVZ) of the embryonic cerebral cortex, its function remains completely unknown. We report finding that Svet1 contains a high proportion of repetitive sequences and maps in the first intron of the putative Netrin receptor gene Unc5d. The direction of transcription of Svet1 is the same as that of Unc5d. The Svet1 RNA was detected in the nucleus but not in the cytoplasm. Both Svet1/Unc5d RNAs and UNC5D protein were localized in the multipolar cells in the SVZ throughout cortical development. These results suggest that Svet1 RNA is part of the sequence of the primary transcript of Unc5d in the nucleus that is spliced out before the mRNA is transported to the cytoplasm. Thus, the previously reported "SVZ-specific expression of the Svet1 RNA" in fact indicates putative involvement of UNC5D signaling in the multipolar migrating cells.

AB - Although Svet1 RNA is a widely used marker for the subventricular zone (SVZ) of the embryonic cerebral cortex, its function remains completely unknown. We report finding that Svet1 contains a high proportion of repetitive sequences and maps in the first intron of the putative Netrin receptor gene Unc5d. The direction of transcription of Svet1 is the same as that of Unc5d. The Svet1 RNA was detected in the nucleus but not in the cytoplasm. Both Svet1/Unc5d RNAs and UNC5D protein were localized in the multipolar cells in the SVZ throughout cortical development. These results suggest that Svet1 RNA is part of the sequence of the primary transcript of Unc5d in the nucleus that is spliced out before the mRNA is transported to the cytoplasm. Thus, the previously reported "SVZ-specific expression of the Svet1 RNA" in fact indicates putative involvement of UNC5D signaling in the multipolar migrating cells.

KW - Cerebral cortex

KW - Multipolar migration

KW - Subventricular zone

KW - Svet1

KW - Unc5d

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