The COUP-TFII/neuropilin-2 is a molecular switch steering diencephalon-derived GABAergic neurons in the developing mouse brain

Shigeaki Kanatani, Takao Honda, Michihiko Aramaki, Kanehiro Hayashi, Kenichiro Kubo, Mami Ishida, Daisuke H. Tanaka, Takeshi Kawauchi, Katsutoshi Sekine, Sayaka Kusuzawa, Takahiko Kawasaki, Tatsumi Hirata, Hidenori Tabata, Per Uhlén, Kazunori Nakajima

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The preoptic area (POa) of the rostral diencephalon supplies the neocortex and the amygdala with GABAergic neurons in the developing mouse brain. However, the molecular mechanisms that determine the pathway and destinations of POa-derived neurons have not yet been identified. Here we show that Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII)-induced expression of Neuropilin-2 (Nrp2) and its down-regulation control the destination of POa-derived GABAergic neurons. Initially, a majority of the POa-derived migrating neurons express COUP-TFII and form a caudal migratory stream toward the caudal subpallium. When a subpopulation of cells steers toward the neocortex, they exhibit decreased expression of COUP-TFII and Nrp2. The present findings show that suppression of COUP-TFII/Nrp2 changed the destination of the cells into the neocortex, whereas overexpression of COUP-TFII/Nrp2 caused cells to end up in the medial part of the amygdala. Taken together, these results reveal that COUP-TFII/Nrp2 is a molecular switch determining the pathway and destination of migrating GABAergic neurons born in the POa.

Original languageEnglish
Pages (from-to)E4985-E4994
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number36
DOIs
Publication statusPublished - 2015 Sep 8

Fingerprint

COUP Transcription Factor II
Neuropilin-2
GABAergic Neurons
Diencephalon
Preoptic Area
Neocortex
Brain
Amygdala
Neurons
Down-Regulation

Keywords

  • Amygdala
  • Caudal migratory stream
  • Cortex
  • COUP-TFII/neuropilin-2
  • Preoptic area

ASJC Scopus subject areas

  • General

Cite this

The COUP-TFII/neuropilin-2 is a molecular switch steering diencephalon-derived GABAergic neurons in the developing mouse brain. / Kanatani, Shigeaki; Honda, Takao; Aramaki, Michihiko; Hayashi, Kanehiro; Kubo, Kenichiro; Ishida, Mami; Tanaka, Daisuke H.; Kawauchi, Takeshi; Sekine, Katsutoshi; Kusuzawa, Sayaka; Kawasaki, Takahiko; Hirata, Tatsumi; Tabata, Hidenori; Uhlén, Per; Nakajima, Kazunori.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 36, 08.09.2015, p. E4985-E4994.

Research output: Contribution to journalArticle

Kanatani, Shigeaki ; Honda, Takao ; Aramaki, Michihiko ; Hayashi, Kanehiro ; Kubo, Kenichiro ; Ishida, Mami ; Tanaka, Daisuke H. ; Kawauchi, Takeshi ; Sekine, Katsutoshi ; Kusuzawa, Sayaka ; Kawasaki, Takahiko ; Hirata, Tatsumi ; Tabata, Hidenori ; Uhlén, Per ; Nakajima, Kazunori. / The COUP-TFII/neuropilin-2 is a molecular switch steering diencephalon-derived GABAergic neurons in the developing mouse brain. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 36. pp. E4985-E4994.
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