The coxsackievirus-adenovirus receptor protein as a cell adhesion molecule in the developing mouse brain

Takao Honda, Hiroshi Saitoh, Masayoshi Masuko, Takako Katagiri-Abe, Kei Tominaga, Ikuo Kozakai, Kazuo Kobayashi, Toshiro Kumanishi, Yuichi G. Watanabe, Shoji Odani, Ryozo Kuwano

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179 Citations (Scopus)

Abstract

In an attempt to elucidate the molecular mechanisms underlying neuro- network formation in the developing brain, we analyzed 130 proteolytic cleavage peptides of membrane proteins purified from newborn mouse brains. We describe here the characterization of a membrane protein with an apparent molecular mass of 46 kDa, a member of the immunoglobulin superfamily of which the cDNA sequence was recently reported, encoding the mouse homologue of the human coxsackievirus and adenovirus receptor (mCAR). Western and Northern blot analyses demonstrated the abundant expression of mCAR in the mouse brain, the highest level being observed in the newborn mouse brain, and its expression was detected in embryos as early as at 10.5 days post-coitus (dpc), but decreased rapidly after birth. On in situ hybridization, mCAR mRNA expression was observed throughout the newborn mouse brain. In primary neurons from the hippocampi of mouse embryos the expression of mCAR was observed throughout the cells including those in growth cones on immunohistochemistry. In order to determine whether or not mCAR is involved in cell adhesion, aggregation assays were carried out. C6 cells transfected with mCAR cDNA aggregated homophilically, which was inhibited by specific antibodies against the extracellular domain of mCAR. In addition to its action as a virus receptor, mCAR may function naturally as an adhesion molecule involved in neuro-network formation in the developing nervous system. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)19-28
Number of pages10
JournalMolecular Brain Research
Volume77
Issue number1
DOIs
Publication statusPublished - 2000 Apr 14

Keywords

  • Adhesion molecule
  • Coxsackievirus and adenovirus receptor
  • In situ hybridization
  • Nerve growth cone
  • Subcellular localization

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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    Honda, T., Saitoh, H., Masuko, M., Katagiri-Abe, T., Tominaga, K., Kozakai, I., Kobayashi, K., Kumanishi, T., Watanabe, Y. G., Odani, S., & Kuwano, R. (2000). The coxsackievirus-adenovirus receptor protein as a cell adhesion molecule in the developing mouse brain. Molecular Brain Research, 77(1), 19-28. https://doi.org/10.1016/S0169-328X(00)00036-X