The CpG-specific methylase Sssl has topoisomerase activity in the presence of Mg2+

Koichi Matsuo, John Silke, Kostadin Gramatikoff, Walter Schaffner

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A prokaryotic CpG-specific methylase from Spiroplasma, Sssl methylase, is now widely used to study the effect of CpG methylation in mammalian cells, and can processively modify cytosines in CpG dinucleotides in the absence of Mg2+. In the presence of Mg2+, we found (i) that the methylation reaction is distributive rather than processive as a result of the decreased affinity of Sssl methylase for DNA, and (ii) that a type I-like topoisomerase activity is present in Sssl methylase preparations. This topoisomerase activity was still present in Sssl methylase further purified by either SDS-polyacrylamide or isoelectric focusing gel electrophoresis. We show that methylase and topoisomerase activities are not functionally interdependent, since conditions exist where only one or the other enzymatic activity is detectable. The catalytic domains of Sssl methylase and prokaryotic topoisomerases show similarity at the amino acid level, further supporting the idea that the topoisomerase activity is a genuine activity of Sssl methylase. Mycoplasmas, including Spiroplasma, have the smallest genomes of all living organisms; thus, this condensation of two enzymatic activites into the same protein may be a result of genome economy, and may also have functional implications for the mechanism of methylation.

Original languageEnglish
Pages (from-to)5354-5359
Number of pages6
JournalNucleic Acids Research
Volume22
Issue number24
Publication statusPublished - 1994 Dec 11
Externally publishedYes

Fingerprint

Methylation
Spiroplasma
Genes
Genome
Type I DNA Topoisomerase
Mycoplasma
Cytosine
Isoelectric Focusing
Polyacrylates
Electrophoresis
Amino acids
Condensation
Catalytic Domain
DNA
Gels
Cells
Proteins
Amino Acids
Affine transformation
DNA modification methylase SssI

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Matsuo, K., Silke, J., Gramatikoff, K., & Schaffner, W. (1994). The CpG-specific methylase Sssl has topoisomerase activity in the presence of Mg2+. Nucleic Acids Research, 22(24), 5354-5359.

The CpG-specific methylase Sssl has topoisomerase activity in the presence of Mg2+. / Matsuo, Koichi; Silke, John; Gramatikoff, Kostadin; Schaffner, Walter.

In: Nucleic Acids Research, Vol. 22, No. 24, 11.12.1994, p. 5354-5359.

Research output: Contribution to journalArticle

Matsuo, K, Silke, J, Gramatikoff, K & Schaffner, W 1994, 'The CpG-specific methylase Sssl has topoisomerase activity in the presence of Mg2+', Nucleic Acids Research, vol. 22, no. 24, pp. 5354-5359.
Matsuo, Koichi ; Silke, John ; Gramatikoff, Kostadin ; Schaffner, Walter. / The CpG-specific methylase Sssl has topoisomerase activity in the presence of Mg2+. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 24. pp. 5354-5359.
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