The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P

Sho Miyazaki, Takumi Katsumata, Masahiro Natsume, Hiroshi Kawaide

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC 50 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC 50 0.26 μM). Crown

Original languageEnglish
Pages (from-to)1879-1883
Number of pages5
JournalFEBS Letters
Volume585
Issue number12
DOIs
Publication statusPublished - 2011 Jun 23
Externally publishedYes

Fingerprint

Bryopsida
Kaurane Diterpenes
Biosynthesis
Gibberellins
Bryophyta
Gene encoding
Enzyme activity
Cytochromes
Crowns
Recombinant Proteins
Arabidopsis
Yeast
Yeasts
Oxidation
uniconazole-P
ent-kaurene oxidase
Enzymes
Genes
kaurenoic acid

Keywords

  • Cytochrome P450
  • Diterpene
  • ent-Kaurene oxidase
  • ent-Kaurenoic acid
  • Gibberellin
  • Physcomitrella patens

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P. / Miyazaki, Sho; Katsumata, Takumi; Natsume, Masahiro; Kawaide, Hiroshi.

In: FEBS Letters, Vol. 585, No. 12, 23.06.2011, p. 1879-1883.

Research output: Contribution to journalArticle

Miyazaki, Sho ; Katsumata, Takumi ; Natsume, Masahiro ; Kawaide, Hiroshi. / The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P. In: FEBS Letters. 2011 ; Vol. 585, No. 12. pp. 1879-1883.
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