The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P

Sho Miyazaki, Takumi Katsumata, Masahiro Natsume, Hiroshi Kawaide

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC50 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC50 0.26 μM). Crown

Original languageEnglish
Pages (from-to)1879-1883
Number of pages5
JournalFEBS Letters
Volume585
Issue number12
DOIs
Publication statusPublished - 2011 Jun 23

Keywords

  • Cytochrome P450
  • Diterpene
  • Gibberellin
  • Physcomitrella patens
  • ent-Kaurene oxidase
  • ent-Kaurenoic acid

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P'. Together they form a unique fingerprint.

  • Cite this