The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P

Sho Miyazaki; Takumi Katsumata; Masahiro Natsume; Hiroshi Kawaide

doi:10.1016/j.febslet.2011.04.057

The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P

Sho Miyazaki, Takumi Katsumata, Masahiro Natsume, Hiroshi Kawaide

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC₅₀ 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC₅₀ 0.26 μM). Crown

Original language	English
Pages (from-to)	1879-1883
Number of pages	5
Journal	FEBS Letters
Volume	585
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2011.04.057
Publication status	Published - 2011 Jun 23
Externally published	Yes

Keywords

Cytochrome P450
Diterpene
Gibberellin
Physcomitrella patens
ent-Kaurene oxidase
ent-Kaurenoic acid

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2011.04.057

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@article{a3cf86fc7bed410db373aaae4739ec47,

title = "The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P",

abstract = "The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC50 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC50 0.26 μM). Crown",

keywords = "Cytochrome P450, Diterpene, Gibberellin, Physcomitrella patens, ent-Kaurene oxidase, ent-Kaurenoic acid",

author = "Sho Miyazaki and Takumi Katsumata and Masahiro Natsume and Hiroshi Kawaide",

year = "2011",

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doi = "10.1016/j.febslet.2011.04.057",

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AU - Miyazaki, Sho

AU - Katsumata, Takumi

AU - Natsume, Masahiro

AU - Kawaide, Hiroshi

PY - 2011/6/23

Y1 - 2011/6/23

N2 - The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC50 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC50 0.26 μM). Crown

AB - The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC50 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC50 0.26 μM). Crown

KW - Cytochrome P450

KW - Diterpene

KW - Gibberellin

KW - Physcomitrella patens

KW - ent-Kaurene oxidase

KW - ent-Kaurenoic acid

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JF - FEBS Letters

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ER -

The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P

Abstract

Keywords

ASJC Scopus subject areas

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