The detection of IRF-1 promoter polymorphisms and their possible contribution to T helper 1 response in chronic hepatitis C

Hidetsugu Saito, Shinichiro Tada, Kanji Wakabayashi, Nobuhiro Nakamoto, Masahiko Takahashi, Mitsuyasu Nakamura, Hirotoshi Ebinuma, Hiromasa Ishii

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We described the interferon (IFN) regulatory factor-1 (IRF-1) promoter single nucleotide polymorphisms (SNPs), and the clinical and immunologic implications of these SNPs have been investigated. We successfully determined the mutation at -300 of the IRF-1 promoter by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and this mutation linked with other mutations in the promoter region. In our Japanese population, the frequency of the type -300*A/A was 11.9%, type A/G was 54.2%, and type G/G was 33.9%. We found no significant difference without IFN stimulation in the production levels of IFN-γ and interleukin-10 (IL-10) from peripheral blood mononuclear cells (PBMC) between subjects with -300*A/A and those with other types. IFN-α stimulation, however, increased the levels of IFN-γ significantly and decreased the IL-10 production level significantly only in the subject with -300*A/A type. Flow cytometric analysis showed that the Th1-type CD4+ cell population was significantly increased by IFN-β administration only in the patient with chronic hepatitis C with -300*A/A type. These results suggest that the IRF-1 promoter SNP types are positively involved in Th1-type response and, consequently, the -300*A/A type may be beneficial for viral elimination in chronic hepatitis C and IFN therapy.

Original languageEnglish
Pages (from-to)693-700
Number of pages8
JournalJournal of Interferon and Cytokine Research
Volume22
Issue number6
DOIs
Publication statusPublished - 2002 Sep 27

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

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