The detergent-insoluble microdomains, rafts, can be used as an effective immunogen

Yohko U. Katagiri, Hideki Nakajima, Ban Sato, Yoshitaka Miyagawa, Yasuomi Horiuchi, Hajime Okita, Junichiro Fujimoto, Nobutaka Kiyokawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Detergent-insoluble microdomains, or rafts, act as a platform to transduce signals from the extracellular space into the cytoplasm. In the process of developing monoclonal antibodies against raft molecules for the purpose of studying the molecular mechanism of raft-mediated signaling, we observed the uniqueness and certain advantages of immunization with rafts. Simple subcutaneous injection of mice with a phosphate-buffered saline (PBS) suspension of rafts without mixing with Freund's adjuvant made it possible to increase the titer of antiserum reacting with raft components. Interestingly, injection of rafts prepared from certain specific cell lines induced monoglycolipid-specific antibodies. Furthermore, antibodies were produced by raft-immunization of even syngeneic mice. Our findings suggest that this phenomenon does not represent a breakdown of immunological self-tolerance, but typical immune reactions accompanying the class switch from IgM antibodies to IgG antibodies.

Original languageEnglish
Pages (from-to)495-501
Number of pages7
JournalGlycoconjugate Journal
Volume25
Issue number6
DOIs
Publication statusPublished - 2008 Aug 1
Externally publishedYes

Keywords

  • Antibody
  • Immune Response
  • Monoglycolipid-specific
  • Raft
  • Syngeneic antigen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Katagiri, Y. U., Nakajima, H., Sato, B., Miyagawa, Y., Horiuchi, Y., Okita, H., Fujimoto, J., & Kiyokawa, N. (2008). The detergent-insoluble microdomains, rafts, can be used as an effective immunogen. Glycoconjugate Journal, 25(6), 495-501. https://doi.org/10.1007/s10719-007-9083-7