Abstract
The nested polymerase chain reaction (PCR) and the loop-mediated isothermal amplification (LAMP) assay were performed to detect and identify toxoplasma parasites in human cerebrospinal fluid (CSF). The performed nested PCR targeting the 18S rDNA using primers generated by Dr. L.D. Sibley instead of the conventionally used primers that target the B1 gene. Toxoplasma gondii-specific LAMP primers targeting both genes were also designed. The clinical sensitivity and specificity were evaluated using clinical CSF samples from 16 patients with toxoplasmic encephalitis (TE) and from 12 patients with other diseases. The 18S rDNA nested PCR showed the highest detection sensitivity limit with a minimum of 1.0 × 10-8 ng/μl. However, sensitivity and specificity of nested PCR with clinical specimens were 50% and 100%, respectively. The sensitivity of molecular diagnosis of TE is not sufficient; therefore, patients clinically suspected of having TE should be treated promptly. This molecular diagnostic tool would restrictively facilitate a definitive diagnosis of TE at an early stage in approximately 50% of patients.
| Original language | English |
|---|---|
| Pages (from-to) | 1194-1195 |
| Number of pages | 2 |
| Journal | Clinical Neurology |
| Volume | 53 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- 18S-rDNA nested PCR
- Loop-mediated isothermal amplification assay
- Toxoplasmic encephalitis
ASJC Scopus subject areas
- Clinical Neurology
Cite this
The diagnosis of toxoplasmic encephalitis by polymerase chain reaction. / Asai, Takashi.
In: Clinical Neurology, Vol. 53, No. 11, 2013, p. 1194-1195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The diagnosis of toxoplasmic encephalitis by polymerase chain reaction
AU - Asai, Takashi
PY - 2013
Y1 - 2013
N2 - The nested polymerase chain reaction (PCR) and the loop-mediated isothermal amplification (LAMP) assay were performed to detect and identify toxoplasma parasites in human cerebrospinal fluid (CSF). The performed nested PCR targeting the 18S rDNA using primers generated by Dr. L.D. Sibley instead of the conventionally used primers that target the B1 gene. Toxoplasma gondii-specific LAMP primers targeting both genes were also designed. The clinical sensitivity and specificity were evaluated using clinical CSF samples from 16 patients with toxoplasmic encephalitis (TE) and from 12 patients with other diseases. The 18S rDNA nested PCR showed the highest detection sensitivity limit with a minimum of 1.0 × 10-8 ng/μl. However, sensitivity and specificity of nested PCR with clinical specimens were 50% and 100%, respectively. The sensitivity of molecular diagnosis of TE is not sufficient; therefore, patients clinically suspected of having TE should be treated promptly. This molecular diagnostic tool would restrictively facilitate a definitive diagnosis of TE at an early stage in approximately 50% of patients.
AB - The nested polymerase chain reaction (PCR) and the loop-mediated isothermal amplification (LAMP) assay were performed to detect and identify toxoplasma parasites in human cerebrospinal fluid (CSF). The performed nested PCR targeting the 18S rDNA using primers generated by Dr. L.D. Sibley instead of the conventionally used primers that target the B1 gene. Toxoplasma gondii-specific LAMP primers targeting both genes were also designed. The clinical sensitivity and specificity were evaluated using clinical CSF samples from 16 patients with toxoplasmic encephalitis (TE) and from 12 patients with other diseases. The 18S rDNA nested PCR showed the highest detection sensitivity limit with a minimum of 1.0 × 10-8 ng/μl. However, sensitivity and specificity of nested PCR with clinical specimens were 50% and 100%, respectively. The sensitivity of molecular diagnosis of TE is not sufficient; therefore, patients clinically suspected of having TE should be treated promptly. This molecular diagnostic tool would restrictively facilitate a definitive diagnosis of TE at an early stage in approximately 50% of patients.
KW - 18S-rDNA nested PCR
KW - Loop-mediated isothermal amplification assay
KW - Toxoplasmic encephalitis
UR - http://www.scopus.com/inward/record.url?scp=84891796100&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891796100&partnerID=8YFLogxK
U2 - 10.5692/clinicalneurol.53.1194
DO - 10.5692/clinicalneurol.53.1194
M3 - Article
VL - 53
SP - 1194
EP - 1195
JO - Clinical Neurology
JF - Clinical Neurology
SN - 0009-918X
IS - 11
ER -