The difference of aquaporin 5 distribution in acinar and ductal cells in lacrimal and parotid glands

Yasumasa Sasaki, Kazuo Tsubota, Jitesh D. Kawedia, Anil G. Menon, Masato Yasui

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Purpose: This study was designed to clarify the physiological function and tissue distribution of aquaporin 5 (AQP5) in the lacrimal and parotid glands. Methods: Saliva and tear volumes were compared in AQP5 knockout (AQP5-/-) mice and wild-type mice. Immunohistochemistry and immunoblot analysis were performed in wild-type and AQP5-/- mice. Results: Immunofluorescence of AQP5 staining showed that AQP5 was localized mainly in the ductal cells rather than in the acinar cells of the lacrimal gland. In contrast, in the parotid gland, AQP5 was observed abundantly in acinar cells with undetectable staining in ductal cells. Tear secretion was not changed in AQP5-/- mouse, although saliva secretion was significantly reduced. Conclusions: AQP5 distribution in acinar cells and ductal cells was completely opposite in the lacrimal and parotid glands. The physiological role of AQP5 might be dependent on the characteristic tissue distribution of the protein in the lacrimal and parotid glands.

Original languageEnglish
Pages (from-to)923-929
Number of pages7
JournalCurrent Eye Research
Volume32
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

Keywords

  • Aquaporin
  • Dry eye
  • Lacrimal gland
  • Parotid gland
  • Tear

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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