The differences of tear function and ocular surface findings in patients with atopic keratoconjunctivitis and vernal keratoconjunctivitis

Y. Hu, Y. Matsumoto, M. Dogru, N. Okada, A. Igarashi, K. Fukagawa, Kazuo Tsubota, H. Fujishima

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: The pathogenesis of the ocular surface disease in atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) has not been fully understood. We tried to clarify the differences in the ocular surface status in patients with AKC, VKC, and healthy control subjects. Methods: Twenty-four eyes of 12 AKC patients, 12 eyes of six VKC patients, and 20 eyes of 10 normal control subjects were studied. The subjects underwent corneal sensitivity measurements, Schirmer test, tear film break-up time (BUT), vital staining of the ocular surface, conjunctival impression and brush cytology. Impression cytology samples underwent periodic acid Schiff staining for goblet cell density, squamous metaplasia grading, and immunohistochemical staining for MUC1, 2, 4, and 5AC. Brush cytology specimens underwent staining for inflammatory cell counting and Real Time PCR for MUC1, 2, 4, and 5AC mRNA expression. Results: The mean BUT, corneal sensitivity, and conjunctival goblet cell density values in AKC patients were significantly lower compared with VKC patients and control subjects. The squamous metaplasia grades in eyes with AKC were significantly higher compared to eyes with VKC and controls. The inflammatory cell response in brush cytology specimens was different between patients with AKC and VKC. Eyes with AKC showed significantly higher MUC1, 2 and 4 and lower MUC5AC mRNA expression compared to eyes with VKC. Conclusions: Differences of the infiltrates, higher level of tear instability, lower corneal sensitivity, up-regulation of MUC1, 2, and 4, and down regulation of MUC5AC were important differential features of the ocular surface disease in AKC compared with VKC.

Original languageEnglish
Pages (from-to)917-925
Number of pages9
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume62
Issue number8
DOIs
Publication statusPublished - 2007 Aug

Fingerprint

Keratoconjunctivitis
Allergic Conjunctivitis
Tears
Cell Biology
Staining and Labeling
Goblet Cells
Eye Diseases
Metaplasia
Cell Count
Messenger RNA
Periodic Acid
Real-Time Polymerase Chain Reaction
Healthy Volunteers
Up-Regulation
Down-Regulation

Keywords

  • Atopic keratoconjunctivitis
  • Brush cytology
  • Impression cytology
  • Ocular surface mucin
  • Vernal keratoconjunctivitis

ASJC Scopus subject areas

  • Immunology

Cite this

The differences of tear function and ocular surface findings in patients with atopic keratoconjunctivitis and vernal keratoconjunctivitis. / Hu, Y.; Matsumoto, Y.; Dogru, M.; Okada, N.; Igarashi, A.; Fukagawa, K.; Tsubota, Kazuo; Fujishima, H.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 62, No. 8, 08.2007, p. 917-925.

Research output: Contribution to journalArticle

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abstract = "Background: The pathogenesis of the ocular surface disease in atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) has not been fully understood. We tried to clarify the differences in the ocular surface status in patients with AKC, VKC, and healthy control subjects. Methods: Twenty-four eyes of 12 AKC patients, 12 eyes of six VKC patients, and 20 eyes of 10 normal control subjects were studied. The subjects underwent corneal sensitivity measurements, Schirmer test, tear film break-up time (BUT), vital staining of the ocular surface, conjunctival impression and brush cytology. Impression cytology samples underwent periodic acid Schiff staining for goblet cell density, squamous metaplasia grading, and immunohistochemical staining for MUC1, 2, 4, and 5AC. Brush cytology specimens underwent staining for inflammatory cell counting and Real Time PCR for MUC1, 2, 4, and 5AC mRNA expression. Results: The mean BUT, corneal sensitivity, and conjunctival goblet cell density values in AKC patients were significantly lower compared with VKC patients and control subjects. The squamous metaplasia grades in eyes with AKC were significantly higher compared to eyes with VKC and controls. The inflammatory cell response in brush cytology specimens was different between patients with AKC and VKC. Eyes with AKC showed significantly higher MUC1, 2 and 4 and lower MUC5AC mRNA expression compared to eyes with VKC. Conclusions: Differences of the infiltrates, higher level of tear instability, lower corneal sensitivity, up-regulation of MUC1, 2, and 4, and down regulation of MUC5AC were important differential features of the ocular surface disease in AKC compared with VKC.",
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AU - Dogru, M.

AU - Okada, N.

AU - Igarashi, A.

AU - Fukagawa, K.

AU - Tsubota, Kazuo

AU - Fujishima, H.

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