TY - JOUR
T1 - The Direct Boil-LAMP method
T2 - A simple and rapid diagnostic method for cutaneous leishmaniasis
AU - Mikita, Kei
AU - Maeda, Takuya
AU - Yoshikawa, Sachio
AU - Ono, Takeshi
AU - Miyahira, Yasushi
AU - Kawana, Akihiko
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2014/12
Y1 - 2014/12
N2 - Needle biopsy is widely used for the diagnosis of cutaneous leishmaniasis (CL) to obtain specimens for histology and culture. However, the use of such invasive procedures causes discomfort, requires technical expertise, and carries risks of bleeding and iatrogenic infection. Therefore, developing substitutive non-invasive diagnostic tools for CL will help reduce the risk of secondary infection and the exposure of both infected individuals and medical professionals. Here we employed loop-mediated isothermal amplification and boiled swab samples (Direct Boil-LAMP method) from CL model mice to develop a simple and rapid diagnostic method for CL. The detection limit of this procedure was 1.0×103parasites/mL. Accordingly, this substitutive diagnostic method should prove useful for mass screening. In addition, we discuss the potential advantages of using it, particularly in endemic regions where medical resources are limited.
AB - Needle biopsy is widely used for the diagnosis of cutaneous leishmaniasis (CL) to obtain specimens for histology and culture. However, the use of such invasive procedures causes discomfort, requires technical expertise, and carries risks of bleeding and iatrogenic infection. Therefore, developing substitutive non-invasive diagnostic tools for CL will help reduce the risk of secondary infection and the exposure of both infected individuals and medical professionals. Here we employed loop-mediated isothermal amplification and boiled swab samples (Direct Boil-LAMP method) from CL model mice to develop a simple and rapid diagnostic method for CL. The detection limit of this procedure was 1.0×103parasites/mL. Accordingly, this substitutive diagnostic method should prove useful for mass screening. In addition, we discuss the potential advantages of using it, particularly in endemic regions where medical resources are limited.
KW - Cutaneous leishmaniasis
KW - Diagnosis
KW - Direct Boil-LAMP
KW - LAMP
KW - Leishmania major
KW - Swab
UR - http://www.scopus.com/inward/record.url?scp=84905901916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84905901916&partnerID=8YFLogxK
U2 - 10.1016/j.parint.2014.07.007
DO - 10.1016/j.parint.2014.07.007
M3 - Article
C2 - 25086375
AN - SCOPUS:84905901916
SN - 1383-5769
VL - 63
SP - 785
EP - 789
JO - Parasitology International
JF - Parasitology International
IS - 6
ER -
