The Direct Boil-LAMP method

A simple and rapid diagnostic method for cutaneous leishmaniasis

Kei Mikita, Takuya Maeda, Sachio Yoshikawa, Takeshi Ono, Yasushi Miyahira, Akihiko Kawana

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Needle biopsy is widely used for the diagnosis of cutaneous leishmaniasis (CL) to obtain specimens for histology and culture. However, the use of such invasive procedures causes discomfort, requires technical expertise, and carries risks of bleeding and iatrogenic infection. Therefore, developing substitutive non-invasive diagnostic tools for CL will help reduce the risk of secondary infection and the exposure of both infected individuals and medical professionals. Here we employed loop-mediated isothermal amplification and boiled swab samples (Direct Boil-LAMP method) from CL model mice to develop a simple and rapid diagnostic method for CL. The detection limit of this procedure was 1.0×103parasites/mL. Accordingly, this substitutive diagnostic method should prove useful for mass screening. In addition, we discuss the potential advantages of using it, particularly in endemic regions where medical resources are limited.

Original languageEnglish
Pages (from-to)785-789
Number of pages5
JournalParasitology International
Volume63
Issue number6
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Furunculosis
Cutaneous Leishmaniasis
Professional Competence
Mass Screening
Needle Biopsy
Coinfection
Limit of Detection
Histology
Hemorrhage
Infection

Keywords

  • Cutaneous leishmaniasis
  • Diagnosis
  • Direct Boil-LAMP
  • LAMP
  • Leishmania major
  • Swab

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Medicine(all)

Cite this

The Direct Boil-LAMP method : A simple and rapid diagnostic method for cutaneous leishmaniasis. / Mikita, Kei; Maeda, Takuya; Yoshikawa, Sachio; Ono, Takeshi; Miyahira, Yasushi; Kawana, Akihiko.

In: Parasitology International, Vol. 63, No. 6, 2014, p. 785-789.

Research output: Contribution to journalArticle

Mikita, Kei ; Maeda, Takuya ; Yoshikawa, Sachio ; Ono, Takeshi ; Miyahira, Yasushi ; Kawana, Akihiko. / The Direct Boil-LAMP method : A simple and rapid diagnostic method for cutaneous leishmaniasis. In: Parasitology International. 2014 ; Vol. 63, No. 6. pp. 785-789.
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