The distinction of underivatized monosaccharides using electrospray ionization ion trap mass spectrometry

Xingyu Zhu, Toshinori Sato

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38 Citations (Scopus)

Abstract

A convenient method for distinguishing underivatized isomeric monosaccharides has been established using electrospray ionization ion trap mass spectrometry (ESI-ITMS). Mass spectra of hexoses (glucose, galactose, and mannose), N-acetylhexosamines (N-acetylglucosamine, N-acetylgalactosamine, and N-acetylmannosamine) and hexosamines (glucosamine, galactosamine, and mannosamine) dissolved in solvent containing 1 mM ammonium acetate were obtained in the positive ion mode. Glucose was distinguished from galactose and mannose in the MS2 spectrum of the [M+NH4]+ ion at m/z 198. The MS3 spectra generated from [MH-NH4-H 2O-NH3]+ at m/z 163 showed that galactose and mannose could be distinguished by the ratio of peak intensities at m/z 145 and 127, while the three N-acetylhexosamine and hexosamine stereochemical isomers could be identified by the relative abundance ratios of product ions observed in MS3 spectra. The investigation of MS and MS2 spectra from complexes of these monosaccharides with Na+ and Pb2+ failed to distinguish these monosaccharide isomers. Therefore, multiple stage mass analysis by ESI-ITMS using either [M+NH4]+ or [M+H]+ was useful to distinguish between the isomers of monosaccharides.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume21
Issue number2
DOIs
Publication statusPublished - 2007

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Electrospray ionization
Monosaccharides
Mass spectrometry
Mannose
Galactose
Isomers
Ions
Hexosamines
Acetylgalactosamine
Glucose
Galactosamine
Hexoses
Acetylglucosamine
Glucosamine
Positive ions

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

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title = "The distinction of underivatized monosaccharides using electrospray ionization ion trap mass spectrometry",
abstract = "A convenient method for distinguishing underivatized isomeric monosaccharides has been established using electrospray ionization ion trap mass spectrometry (ESI-ITMS). Mass spectra of hexoses (glucose, galactose, and mannose), N-acetylhexosamines (N-acetylglucosamine, N-acetylgalactosamine, and N-acetylmannosamine) and hexosamines (glucosamine, galactosamine, and mannosamine) dissolved in solvent containing 1 mM ammonium acetate were obtained in the positive ion mode. Glucose was distinguished from galactose and mannose in the MS2 spectrum of the [M+NH4]+ ion at m/z 198. The MS3 spectra generated from [MH-NH4-H 2O-NH3]+ at m/z 163 showed that galactose and mannose could be distinguished by the ratio of peak intensities at m/z 145 and 127, while the three N-acetylhexosamine and hexosamine stereochemical isomers could be identified by the relative abundance ratios of product ions observed in MS3 spectra. The investigation of MS and MS2 spectra from complexes of these monosaccharides with Na+ and Pb2+ failed to distinguish these monosaccharide isomers. Therefore, multiple stage mass analysis by ESI-ITMS using either [M+NH4]+ or [M+H]+ was useful to distinguish between the isomers of monosaccharides.",
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AU - Sato, Toshinori

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AB - A convenient method for distinguishing underivatized isomeric monosaccharides has been established using electrospray ionization ion trap mass spectrometry (ESI-ITMS). Mass spectra of hexoses (glucose, galactose, and mannose), N-acetylhexosamines (N-acetylglucosamine, N-acetylgalactosamine, and N-acetylmannosamine) and hexosamines (glucosamine, galactosamine, and mannosamine) dissolved in solvent containing 1 mM ammonium acetate were obtained in the positive ion mode. Glucose was distinguished from galactose and mannose in the MS2 spectrum of the [M+NH4]+ ion at m/z 198. The MS3 spectra generated from [MH-NH4-H 2O-NH3]+ at m/z 163 showed that galactose and mannose could be distinguished by the ratio of peak intensities at m/z 145 and 127, while the three N-acetylhexosamine and hexosamine stereochemical isomers could be identified by the relative abundance ratios of product ions observed in MS3 spectra. The investigation of MS and MS2 spectra from complexes of these monosaccharides with Na+ and Pb2+ failed to distinguish these monosaccharide isomers. Therefore, multiple stage mass analysis by ESI-ITMS using either [M+NH4]+ or [M+H]+ was useful to distinguish between the isomers of monosaccharides.

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