Abstract
Double-stranded (ds) RNA induces potent gene silencing, termed RNA interference (RNAi). At an early step in RNAi, an RNaseIII-related enzyme, Dicer (DCR-1), processes long-trigger dsRNA into small interfering RNAs (siRNAs). DCR-1 is also required for processing endogenous regulatory RNAs called miRNAs, but how DCR-1 recognizes its endogenous and foreign substrates is not yet understood. Here we show that the C. elegans RNAi pathway gene, rde-4, encodes a dsRNA binding protein that interacts during RNAi with RNA identical to the trigger dsRNA. RDE-4 protein also interacts in vivo with DCR-1, RDE-1, and a conserved DExH-box helicase. Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing.
| Original language | English |
|---|---|
| Pages (from-to) | 861-871 |
| Number of pages | 11 |
| Journal | Cell |
| Volume | 109 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2002 Jun 28 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-Box helicase to direct RNAi in C. elegans. / Tabara, Hiroaki; Yigit, Erbay; Siomi, Haruhiko; Mello, Craig C.
In: Cell, Vol. 109, No. 7, 28.06.2002, p. 861-871.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-Box helicase to direct RNAi in C. elegans
AU - Tabara, Hiroaki
AU - Yigit, Erbay
AU - Siomi, Haruhiko
AU - Mello, Craig C.
PY - 2002/6/28
Y1 - 2002/6/28
N2 - Double-stranded (ds) RNA induces potent gene silencing, termed RNA interference (RNAi). At an early step in RNAi, an RNaseIII-related enzyme, Dicer (DCR-1), processes long-trigger dsRNA into small interfering RNAs (siRNAs). DCR-1 is also required for processing endogenous regulatory RNAs called miRNAs, but how DCR-1 recognizes its endogenous and foreign substrates is not yet understood. Here we show that the C. elegans RNAi pathway gene, rde-4, encodes a dsRNA binding protein that interacts during RNAi with RNA identical to the trigger dsRNA. RDE-4 protein also interacts in vivo with DCR-1, RDE-1, and a conserved DExH-box helicase. Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing.
AB - Double-stranded (ds) RNA induces potent gene silencing, termed RNA interference (RNAi). At an early step in RNAi, an RNaseIII-related enzyme, Dicer (DCR-1), processes long-trigger dsRNA into small interfering RNAs (siRNAs). DCR-1 is also required for processing endogenous regulatory RNAs called miRNAs, but how DCR-1 recognizes its endogenous and foreign substrates is not yet understood. Here we show that the C. elegans RNAi pathway gene, rde-4, encodes a dsRNA binding protein that interacts during RNAi with RNA identical to the trigger dsRNA. RDE-4 protein also interacts in vivo with DCR-1, RDE-1, and a conserved DExH-box helicase. Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing.
UR - http://www.scopus.com/inward/record.url?scp=0037188904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037188904&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(02)00793-6
DO - 10.1016/S0092-8674(02)00793-6
M3 - Article
C2 - 12110183
AN - SCOPUS:0037188904
VL - 109
SP - 861
EP - 871
JO - Cell
JF - Cell
SN - 0092-8674
IS - 7
ER -