TY - JOUR
T1 - The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-Box helicase to direct RNAi in C. elegans
AU - Tabara, Hiroaki
AU - Yigit, Erbay
AU - Siomi, Haruhiko
AU - Mello, Craig C.
N1 - Funding Information:
We thank the members of our laboratories for helpful discussions; N. Li, L.M. Rivera, and D. Guertin for technical assistance; A. Fire for reagents and helpful discussions; T.H. Shin, A.E. Pasquinelli, and H. Sakamoto for several technical suggestions; J.F. Mello for comments on the manuscript; Y. Kohara and A. Coulson for strains and reagents; the C. elegans Genetics Center (funded by National Institutes of Health National Center for Research Support) for strains; and the PMM core for sequence analysis and peptide mass spectrometry. Research support was provided in part by Japan Society for the Promotion of Science grants to H.T. and H.S. and a National Institutes of Health grant to C.C.M. (GM58800). C.C.M. is a Howard Hughes Medical Institute assistant investigator.
PY - 2002/6/28
Y1 - 2002/6/28
N2 - Double-stranded (ds) RNA induces potent gene silencing, termed RNA interference (RNAi). At an early step in RNAi, an RNaseIII-related enzyme, Dicer (DCR-1), processes long-trigger dsRNA into small interfering RNAs (siRNAs). DCR-1 is also required for processing endogenous regulatory RNAs called miRNAs, but how DCR-1 recognizes its endogenous and foreign substrates is not yet understood. Here we show that the C. elegans RNAi pathway gene, rde-4, encodes a dsRNA binding protein that interacts during RNAi with RNA identical to the trigger dsRNA. RDE-4 protein also interacts in vivo with DCR-1, RDE-1, and a conserved DExH-box helicase. Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing.
AB - Double-stranded (ds) RNA induces potent gene silencing, termed RNA interference (RNAi). At an early step in RNAi, an RNaseIII-related enzyme, Dicer (DCR-1), processes long-trigger dsRNA into small interfering RNAs (siRNAs). DCR-1 is also required for processing endogenous regulatory RNAs called miRNAs, but how DCR-1 recognizes its endogenous and foreign substrates is not yet understood. Here we show that the C. elegans RNAi pathway gene, rde-4, encodes a dsRNA binding protein that interacts during RNAi with RNA identical to the trigger dsRNA. RDE-4 protein also interacts in vivo with DCR-1, RDE-1, and a conserved DExH-box helicase. Our findings suggest a model in which RDE-4 and RDE-1 function together to detect and retain foreign dsRNA and to present this dsRNA to DCR-1 for processing.
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U2 - 10.1016/S0092-8674(02)00793-6
DO - 10.1016/S0092-8674(02)00793-6
M3 - Article
C2 - 12110183
AN - SCOPUS:0037188904
VL - 109
SP - 861
EP - 871
JO - Cell
JF - Cell
SN - 0092-8674
IS - 7
ER -