TY - JOUR
T1 - The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter
AU - Abe, Yoichiro
AU - Goda, Wakami
AU - Ikeshima-Kataoka, Hiroko
AU - Yasui, Masato
N1 - Funding Information:
The authors thank Dr. Dovie Wylie for editing the manuscript; Ms. Manae Imamura and Ms. Yoshiko Yagyu for expert assistance; Collaborative Research Resources, School of Medicine, Keio University for technical assistance; and all members of the Department of Pharmacology, School of Medicine, Keio University for cooperation. This work was supported by grants from the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (C) (22590940); Grant-in-Aid for Challenging Exploratory Research (25670425); Grant-in-Aid for Scientific Research (B) (16H05134); and Keio Gijuku Academic Development Funds.
PY - 2017/12
Y1 - 2017/12
N2 - Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.
AB - Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.
KW - aquaporin-4
KW - astrocytes
KW - enhancer
KW - silencer
KW - transcription
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U2 - 10.1002/1873-3468.12910
DO - 10.1002/1873-3468.12910
M3 - Article
C2 - 29125630
AN - SCOPUS:85035022021
VL - 591
SP - 3906
EP - 3915
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 23
ER -