Abstract
Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.
| Original language | English |
|---|---|
| Pages (from-to) | 3906-3915 |
| Number of pages | 10 |
| Journal | FEBS Letters |
| Volume | 591 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - 2017 Dec 1 |
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Keywords
- aquaporin-4
- astrocytes
- enhancer
- silencer
- transcription
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter. / Abe, Yoichiro; Goda, Wakami; Ikeshima-Kataoka, Hiroko; Yasui, Masato.
In: FEBS Letters, Vol. 591, No. 23, 01.12.2017, p. 3906-3915.Research output: Contribution to journal › Letter
}
TY - JOUR
T1 - The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter
AU - Abe, Yoichiro
AU - Goda, Wakami
AU - Ikeshima-Kataoka, Hiroko
AU - Yasui, Masato
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.
AB - Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.
KW - aquaporin-4
KW - astrocytes
KW - enhancer
KW - silencer
KW - transcription
UR - http://www.scopus.com/inward/record.url?scp=85035022021&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85035022021&partnerID=8YFLogxK
U2 - 10.1002/1873-3468.12910
DO - 10.1002/1873-3468.12910
M3 - Letter
C2 - 29125630
AN - SCOPUS:85035022021
VL - 591
SP - 3906
EP - 3915
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 23
ER -