The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter

Yoichiro Abe, Wakami Goda, Hiroko Ikeshima-Kataoka, Masato Yasui

Research output: Contribution to journalLetter

Abstract

Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.

Original languageEnglish
Pages (from-to)3906-3915
Number of pages10
JournalFEBS Letters
Volume591
Issue number23
DOIs
Publication statusPublished - 2017 Dec 1

Keywords

  • aquaporin-4
  • astrocytes
  • enhancer
  • silencer
  • transcription

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter'. Together they form a unique fingerprint.

  • Cite this