The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter

Yoichiro Abe, Wakami Goda, Hiroko Ikeshima-Kataoka, Masato Yasui

Research output: Contribution to journalLetter

Abstract

Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.

Original languageEnglish
Pages (from-to)3906-3915
Number of pages10
JournalFEBS Letters
Volume591
Issue number23
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Astrocytes
Aquaporin 4
Aquaporins
Neurology
Exons
Assays
Protein Isoforms
Central Nervous System
Genes

Keywords

  • aquaporin-4
  • astrocytes
  • enhancer
  • silencer
  • transcription

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter. / Abe, Yoichiro; Goda, Wakami; Ikeshima-Kataoka, Hiroko; Yasui, Masato.

In: FEBS Letters, Vol. 591, No. 23, 01.12.2017, p. 3906-3915.

Research output: Contribution to journalLetter

Abe, Yoichiro ; Goda, Wakami ; Ikeshima-Kataoka, Hiroko ; Yasui, Masato. / The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter. In: FEBS Letters. 2017 ; Vol. 591, No. 23. pp. 3906-3915.
@article{9133a74023fd4a9384c8c3ab2d824bd4,
title = "The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter",
abstract = "Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.",
keywords = "aquaporin-4, astrocytes, enhancer, silencer, transcription",
author = "Yoichiro Abe and Wakami Goda and Hiroko Ikeshima-Kataoka and Masato Yasui",
year = "2017",
month = "12",
day = "1",
doi = "10.1002/1873-3468.12910",
language = "English",
volume = "591",
pages = "3906--3915",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "23",

}

TY - JOUR

T1 - The dual effects of the astrocyte-specific enhancer of the AQP4 M1 promoter

AU - Abe, Yoichiro

AU - Goda, Wakami

AU - Ikeshima-Kataoka, Hiroko

AU - Yasui, Masato

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.

AB - Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lacked enhancers functioning in astrocytes. When the astrocyte-specific enhancer (ASE) of the M1 promoter is connected to the putative M23 core promoter, it also works in astrocytes. Importantly, the ASE inhibits downstream promoter activity in NIH3T3 cells, indicating that the ASE also functions as a silencer in cells lacking aquaporin-4.

KW - aquaporin-4

KW - astrocytes

KW - enhancer

KW - silencer

KW - transcription

UR - http://www.scopus.com/inward/record.url?scp=85035022021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85035022021&partnerID=8YFLogxK

U2 - 10.1002/1873-3468.12910

DO - 10.1002/1873-3468.12910

M3 - Letter

C2 - 29125630

AN - SCOPUS:85035022021

VL - 591

SP - 3906

EP - 3915

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 23

ER -