The dual origin of the peripheral olfactory system: Placode and neural crest

Hiroyuki Katoh, Shinsuke Shibata, Kimiko Fukuda, Momoka Sato, Etsuko Satoh, Narihito Nagoshi, Takeo Minematsu, Yumi Matsuzaki, Chihiro Akazawa, Yoshiaki Toyama, Masaya Nakamura, Hideyuki Okano

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Abstract. Background: The olfactory epithelium (OE) has a unique capacity for continuous neurogenesis, extending axons to the olfactory bulb with the assistance of olfactory ensheathing cells (OECs). The OE and OECs have been believed to develop solely from the olfactory placode, while the neural crest (NC) cells have been believed to contribute only the underlying structural elements of the olfactory system. In order to further elucidate the role of NC cells in olfactory development, we examined the olfactory system in the transgenic mice Wnt1-Cre/Floxed-EGFP and P0-Cre/Floxed-EGFP, in which migrating NC cells and its descendents permanently express GFP, and conducted transposon-mediated cell lineage tracing studies in chick embryos. Results: Examination of these transgenic mice revealed GFP-positive cells in the OE, demonstrating that NC-derived cells give rise to OE cells with morphologic and antigenic properties identical to placode-derived cells. OECs were also positive for GFP, confirming their NC origin. Cell lineage tracing studies performed in chick embryos confirmed the migration of NC cells into the OE. Furthermore, spheres cultured from the dissociated cells of the olfactory mucosa demonstrated self-renewal and trilineage differentiation capacities (neurons, glial cells, and myofibroblasts), demonstrating the presence of NC progenitors in the olfactory mucosa. Conclusion: Our data demonstrates that the NC plays a larger role in the development of the olfactory system than previously believed, and suggests that NC-derived cells may in part be responsible for the remarkable capacity of the OE for neurogenesis and regeneration.

Original languageEnglish
Article number34
JournalMolecular brain
Volume4
Issue number1
DOIs
Publication statusPublished - 2011

Keywords

  • P0-Cre
  • Sox10
  • Wnt1-Cre
  • chick embryo
  • neural crest
  • neural crest progenitor cell
  • olfactory ensheathing cell
  • olfactory placode

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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