The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system

Hideyuki Ishizuka, Takanobu Maezawa, Junpei Kawauchi, Hanae Nodono, Yukako Hirao, Osamu Nishimura, Haruka Nakagawa, Kiyono Sekii, Kenta Tasaka, Hiroshi Tarui, Kiyokazu Agata, Motonori Hoshi, Kazuya Kobayashi, Yasubumi Sakakibara, Midori Matsumoto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/ planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalZoological Science
Volume24
Issue number1
DOIs
Publication statusPublished - 2007 Jan

Fingerprint

Dugesia
reproductive system
expressed sequence tags
Planaria
unigenes
cDNA libraries
actin
germ cells
testes
proteins
cells
methodology

Keywords

  • Asexual-sexual switch
  • EST analysis
  • Gene ontology
  • Planarian
  • Sexualization

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system. / Ishizuka, Hideyuki; Maezawa, Takanobu; Kawauchi, Junpei; Nodono, Hanae; Hirao, Yukako; Nishimura, Osamu; Nakagawa, Haruka; Sekii, Kiyono; Tasaka, Kenta; Tarui, Hiroshi; Agata, Kiyokazu; Hoshi, Motonori; Kobayashi, Kazuya; Sakakibara, Yasubumi; Matsumoto, Midori.

In: Zoological Science, Vol. 24, No. 1, 01.2007, p. 31-37.

Research output: Contribution to journalArticle

Ishizuka, H, Maezawa, T, Kawauchi, J, Nodono, H, Hirao, Y, Nishimura, O, Nakagawa, H, Sekii, K, Tasaka, K, Tarui, H, Agata, K, Hoshi, M, Kobayashi, K, Sakakibara, Y & Matsumoto, M 2007, 'The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system', Zoological Science, vol. 24, no. 1, pp. 31-37. https://doi.org/10.2108/zsj.24.31
Ishizuka, Hideyuki ; Maezawa, Takanobu ; Kawauchi, Junpei ; Nodono, Hanae ; Hirao, Yukako ; Nishimura, Osamu ; Nakagawa, Haruka ; Sekii, Kiyono ; Tasaka, Kenta ; Tarui, Hiroshi ; Agata, Kiyokazu ; Hoshi, Motonori ; Kobayashi, Kazuya ; Sakakibara, Yasubumi ; Matsumoto, Midori. / The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system. In: Zoological Science. 2007 ; Vol. 24, No. 1. pp. 31-37.
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