Abstract
The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/ planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians.
| Original language | English |
|---|---|
| Pages (from-to) | 31-37 |
| Number of pages | 7 |
| Journal | Zoological Science |
| Volume | 24 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 Jan |
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Keywords
- Asexual-sexual switch
- EST analysis
- Gene ontology
- Planarian
- Sexualization
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system. / Ishizuka, Hideyuki; Maezawa, Takanobu; Kawauchi, Junpei; Nodono, Hanae; Hirao, Yukako; Nishimura, Osamu; Nakagawa, Haruka; Sekii, Kiyono; Tasaka, Kenta; Tarui, Hiroshi; Agata, Kiyokazu; Hoshi, Motonori; Kobayashi, Kazuya; Sakakibara, Yasubumi; Matsumoto, Midori.
In: Zoological Science, Vol. 24, No. 1, 01.2007, p. 31-37.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system
AU - Ishizuka, Hideyuki
AU - Maezawa, Takanobu
AU - Kawauchi, Junpei
AU - Nodono, Hanae
AU - Hirao, Yukako
AU - Nishimura, Osamu
AU - Nakagawa, Haruka
AU - Sekii, Kiyono
AU - Tasaka, Kenta
AU - Tarui, Hiroshi
AU - Agata, Kiyokazu
AU - Hoshi, Motonori
AU - Kobayashi, Kazuya
AU - Sakakibara, Yasubumi
AU - Matsumoto, Midori
PY - 2007/1
Y1 - 2007/1
N2 - The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/ planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians.
AB - The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/ planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians.
KW - Asexual-sexual switch
KW - EST analysis
KW - Gene ontology
KW - Planarian
KW - Sexualization
UR - http://www.scopus.com/inward/record.url?scp=34247540924&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247540924&partnerID=8YFLogxK
U2 - 10.2108/zsj.24.31
DO - 10.2108/zsj.24.31
M3 - Article
VL - 24
SP - 31
EP - 37
JO - Zoological Science
JF - Zoological Science
SN - 0289-0003
IS - 1
ER -