The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas

Yae Kanai, Saori Ushijima, Ai Min Hui, Atsushi Ochiai, Hitoshi Tsuda, Michiie Sakamoto, Setsuo Hirohashi

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

Our study was designed to clarify the significance of silencing the E- cadherin gene, which is located on 16q22.1, due to CpG methylation during hepatocarcinogenesis. The CpG methylation status of primary hepatocellular carcinomas (HCCs) and corresponding liver tissues showing chronic hepatitis or cirrhosis, which are widely considered to be precancerous conditions, were assessed by digesting DNA with methylation-sensitive and non-sensitive restriction enzymes. CpG methylation around the promoter region of the E- cadherin gene was detected in 46% of liver tissues showing chronic hepatitis or cirrhosis and 67% of HCCs examined. Immunohistochemical examination revealed reduced E-cadherin expression in 59% of HCCs examined. CpG methylation around the promoter region correlated significantly with reduced E-cadherin expression in HCCs (p < 0.05). CpG methylation around the promoter region, which increases during the progression from a precancerous condition to HCC, may participate in hepatocarcinogenesis through reduction of E- cadherin expression, resulting in loss of intercellular adhesiveness and destruction of tissue morphology.

Original languageEnglish
Pages (from-to)355-359
Number of pages5
JournalInternational Journal of Cancer
Volume71
Issue number3
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Cadherins
Methylation
Hepatocellular Carcinoma
Genetic Promoter Regions
Precancerous Conditions
Genes
Chronic Hepatitis
Fibrosis
Adhesiveness
Liver
DNA Methylation
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas. / Kanai, Yae; Ushijima, Saori; Hui, Ai Min; Ochiai, Atsushi; Tsuda, Hitoshi; Sakamoto, Michiie; Hirohashi, Setsuo.

In: International Journal of Cancer, Vol. 71, No. 3, 1997, p. 355-359.

Research output: Contribution to journalArticle

Kanai, Yae ; Ushijima, Saori ; Hui, Ai Min ; Ochiai, Atsushi ; Tsuda, Hitoshi ; Sakamoto, Michiie ; Hirohashi, Setsuo. / The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas. In: International Journal of Cancer. 1997 ; Vol. 71, No. 3. pp. 355-359.
@article{17df070b39234d5a830604adbc65d435,
title = "The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas",
abstract = "Our study was designed to clarify the significance of silencing the E- cadherin gene, which is located on 16q22.1, due to CpG methylation during hepatocarcinogenesis. The CpG methylation status of primary hepatocellular carcinomas (HCCs) and corresponding liver tissues showing chronic hepatitis or cirrhosis, which are widely considered to be precancerous conditions, were assessed by digesting DNA with methylation-sensitive and non-sensitive restriction enzymes. CpG methylation around the promoter region of the E- cadherin gene was detected in 46{\%} of liver tissues showing chronic hepatitis or cirrhosis and 67{\%} of HCCs examined. Immunohistochemical examination revealed reduced E-cadherin expression in 59{\%} of HCCs examined. CpG methylation around the promoter region correlated significantly with reduced E-cadherin expression in HCCs (p < 0.05). CpG methylation around the promoter region, which increases during the progression from a precancerous condition to HCC, may participate in hepatocarcinogenesis through reduction of E- cadherin expression, resulting in loss of intercellular adhesiveness and destruction of tissue morphology.",
author = "Yae Kanai and Saori Ushijima and Hui, {Ai Min} and Atsushi Ochiai and Hitoshi Tsuda and Michiie Sakamoto and Setsuo Hirohashi",
year = "1997",
doi = "10.1002/(SICI)1097-0215(19970502)71:3<355::AID-IJC8>3.0.CO;2-X",
language = "English",
volume = "71",
pages = "355--359",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas

AU - Kanai, Yae

AU - Ushijima, Saori

AU - Hui, Ai Min

AU - Ochiai, Atsushi

AU - Tsuda, Hitoshi

AU - Sakamoto, Michiie

AU - Hirohashi, Setsuo

PY - 1997

Y1 - 1997

N2 - Our study was designed to clarify the significance of silencing the E- cadherin gene, which is located on 16q22.1, due to CpG methylation during hepatocarcinogenesis. The CpG methylation status of primary hepatocellular carcinomas (HCCs) and corresponding liver tissues showing chronic hepatitis or cirrhosis, which are widely considered to be precancerous conditions, were assessed by digesting DNA with methylation-sensitive and non-sensitive restriction enzymes. CpG methylation around the promoter region of the E- cadherin gene was detected in 46% of liver tissues showing chronic hepatitis or cirrhosis and 67% of HCCs examined. Immunohistochemical examination revealed reduced E-cadherin expression in 59% of HCCs examined. CpG methylation around the promoter region correlated significantly with reduced E-cadherin expression in HCCs (p < 0.05). CpG methylation around the promoter region, which increases during the progression from a precancerous condition to HCC, may participate in hepatocarcinogenesis through reduction of E- cadherin expression, resulting in loss of intercellular adhesiveness and destruction of tissue morphology.

AB - Our study was designed to clarify the significance of silencing the E- cadherin gene, which is located on 16q22.1, due to CpG methylation during hepatocarcinogenesis. The CpG methylation status of primary hepatocellular carcinomas (HCCs) and corresponding liver tissues showing chronic hepatitis or cirrhosis, which are widely considered to be precancerous conditions, were assessed by digesting DNA with methylation-sensitive and non-sensitive restriction enzymes. CpG methylation around the promoter region of the E- cadherin gene was detected in 46% of liver tissues showing chronic hepatitis or cirrhosis and 67% of HCCs examined. Immunohistochemical examination revealed reduced E-cadherin expression in 59% of HCCs examined. CpG methylation around the promoter region correlated significantly with reduced E-cadherin expression in HCCs (p < 0.05). CpG methylation around the promoter region, which increases during the progression from a precancerous condition to HCC, may participate in hepatocarcinogenesis through reduction of E- cadherin expression, resulting in loss of intercellular adhesiveness and destruction of tissue morphology.

UR - http://www.scopus.com/inward/record.url?scp=0030920634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030920634&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(19970502)71:3<355::AID-IJC8>3.0.CO;2-X

DO - 10.1002/(SICI)1097-0215(19970502)71:3<355::AID-IJC8>3.0.CO;2-X

M3 - Article

C2 - 9139867

AN - SCOPUS:0030920634

VL - 71

SP - 355

EP - 359

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 3

ER -