The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas

Yae Kanai, Saori Ushijima, Ai Min Hui, Atsushi Ochiai, Hitoshi Tsuda, Michiie Sakamoto, Setsuo Hirohashi

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238 Citations (Scopus)


Our study was designed to clarify the significance of silencing the E- cadherin gene, which is located on 16q22.1, due to CpG methylation during hepatocarcinogenesis. The CpG methylation status of primary hepatocellular carcinomas (HCCs) and corresponding liver tissues showing chronic hepatitis or cirrhosis, which are widely considered to be precancerous conditions, were assessed by digesting DNA with methylation-sensitive and non-sensitive restriction enzymes. CpG methylation around the promoter region of the E- cadherin gene was detected in 46% of liver tissues showing chronic hepatitis or cirrhosis and 67% of HCCs examined. Immunohistochemical examination revealed reduced E-cadherin expression in 59% of HCCs examined. CpG methylation around the promoter region correlated significantly with reduced E-cadherin expression in HCCs (p < 0.05). CpG methylation around the promoter region, which increases during the progression from a precancerous condition to HCC, may participate in hepatocarcinogenesis through reduction of E- cadherin expression, resulting in loss of intercellular adhesiveness and destruction of tissue morphology.

Original languageEnglish
Pages (from-to)355-359
Number of pages5
JournalInternational Journal of Cancer
Issue number3
Publication statusPublished - 1997 Jun 9
Externally publishedYes


ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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