The E3 ligase VHL promotes follicular helper T cell differentiation via glycolytic-epigenetic control

Yangyang Zhu, Yanxia Zhao, Le Zou, Danfeng Zhang, Daisuke Aki, Yun Cai Liu

Research output: Contribution to journalArticle

Abstract

Follicular helper T (Tfh) cells are essential for germinal center formation and effective humoral immunity, which undergo different stages of development to become fully polarized. However, the detailed mechanisms of their regulation remain unsolved. Here we found that the E3 ubiquitin ligase VHL was required for Tfh cell development and function upon acute virus infection or antigen immunization. VHL acted through the hypoxia-inducible factor 1α (HIF-1α)-dependent glycolysis pathway to positively regulate early Tfh cell initiation. The enhanced glycolytic activity due to VHL deficiency was involved in the epigenetic regulation of ICOS expression, a critical molecule for Tfh development. By using an RNA interference screen, we identified the glycolytic enzyme GAPDH as the key target for the reduced ICOS expression via m6A modification. Our results thus demonstrated that the VHL-HIF-1α axis played an important role during the initiation of Tfh cell development through glycolytic-epigenetic reprogramming.

Original languageEnglish
Pages (from-to)1664-1681
Number of pages18
JournalThe Journal of experimental medicine
Volume216
Issue number7
DOIs
Publication statusPublished - 2019 Jul 1

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Ubiquitin-Protein Ligases
Helper-Inducer T-Lymphocytes
Epigenomics
Hypoxia-Inducible Factor 1
Cell Differentiation
Germinal Center
Glycolysis
Virus Diseases
Humoral Immunity
RNA Interference
Immunization
Antigens
Enzymes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

The E3 ligase VHL promotes follicular helper T cell differentiation via glycolytic-epigenetic control. / Zhu, Yangyang; Zhao, Yanxia; Zou, Le; Zhang, Danfeng; Aki, Daisuke; Liu, Yun Cai.

In: The Journal of experimental medicine, Vol. 216, No. 7, 01.07.2019, p. 1664-1681.

Research output: Contribution to journalArticle

Zhu, Yangyang ; Zhao, Yanxia ; Zou, Le ; Zhang, Danfeng ; Aki, Daisuke ; Liu, Yun Cai. / The E3 ligase VHL promotes follicular helper T cell differentiation via glycolytic-epigenetic control. In: The Journal of experimental medicine. 2019 ; Vol. 216, No. 7. pp. 1664-1681.
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