TY - JOUR
T1 - The EAT/mcl-1 gene, an inhibitor of apoptosis, is up-regulated in the early stage of acute myocardial infarction
AU - Matsushita, Kenichi
AU - Umezawa, Akihiro
AU - Iwanaga, Shiro
AU - Oda, Takahiro
AU - Okita, Hajime
AU - Kimura, Kensuke
AU - Shimada, Megumi
AU - Tanaka, Mie
AU - Sano, Makoto
AU - Ogawa, Satoshi
AU - Hata, Jun ichi
N1 - Funding Information:
We are grateful to J. Ozawa, S. Kusakari, H. Abe, and Y. Hashimoto for their technical assistance. We are also grateful to A. Suzuki, T. Ando, F. Urano, M. Fukuma, S. Tajima and S. Matsumura for useful discussion. This work was supported in part by a grant from the Ministry of Education, Science and Culture to J.H. and A.U., by a Keio University Special Grant-in-Aid for Innovative Collaborative Research Project to J.H. and A.U., by Keio Gijuku Fukuzawa Memorial Funds for the Advancement of Education and Research from Keio University to H.O., and by a National Grant-in-Aid for the Establishment of a High-Tech Research Center at Private Universities to J.H.
PY - 1999/11/16
Y1 - 1999/11/16
N2 - EAT/mcl-1 (EAT), a bcl-2-related immediate early gene, is up-regulated at an early stage of differentiation of human embryonal carcinoma cells. Recent studies have revealed that EAT inhibits apoptosis both in vitro and in vivo. In the present study, we demonstrated that the EAT gene was up-regulated in the early stage of rat myocardial infarction. This pattern of up-regulation was apparently different from that of another immediate early gene, c-fos. EAT, an anti-apoptotic molecule, was strongly up-regulated in the non-ischemic region. In contrast, the expression of c-fos was induced in both ischemic and non-ischemic regions, and was higher in the ischemic region. Apoptosis of cardiomyocytes is currently thought to significantly contribute to acute myocardial infarction. We detected cardiomyocyte apoptosis by gel electrophoresis of genomic DNA and in situ nick end labeling in the ischemic region, but not in the non-ischemic region. As an inhibitor of apoptosis, EAT may play a role in the protection of cardiomyocytes in the early stage of acute myocardial infarction. Copyright (C) 1999 Elsevier Science B.V.
AB - EAT/mcl-1 (EAT), a bcl-2-related immediate early gene, is up-regulated at an early stage of differentiation of human embryonal carcinoma cells. Recent studies have revealed that EAT inhibits apoptosis both in vitro and in vivo. In the present study, we demonstrated that the EAT gene was up-regulated in the early stage of rat myocardial infarction. This pattern of up-regulation was apparently different from that of another immediate early gene, c-fos. EAT, an anti-apoptotic molecule, was strongly up-regulated in the non-ischemic region. In contrast, the expression of c-fos was induced in both ischemic and non-ischemic regions, and was higher in the ischemic region. Apoptosis of cardiomyocytes is currently thought to significantly contribute to acute myocardial infarction. We detected cardiomyocyte apoptosis by gel electrophoresis of genomic DNA and in situ nick end labeling in the ischemic region, but not in the non-ischemic region. As an inhibitor of apoptosis, EAT may play a role in the protection of cardiomyocytes in the early stage of acute myocardial infarction. Copyright (C) 1999 Elsevier Science B.V.
KW - Apoptosis
KW - Bcl-2
KW - EAT/mcl-1
KW - Ischemia
KW - Myocardial infarction
KW - c-Fos
UR - http://www.scopus.com/inward/record.url?scp=0032728996&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032728996&partnerID=8YFLogxK
U2 - 10.1016/S0304-4165(99)00149-X
DO - 10.1016/S0304-4165(99)00149-X
M3 - Article
C2 - 10564761
AN - SCOPUS:0032728996
SN - 0006-3002
VL - 1472
SP - 471
EP - 478
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 3
ER -