The EAT/mcl-1 gene, an inhibitor of apoptosis, is up-regulated in the early stage of acute myocardial infarction

Kenichi Matsushita, Akihiro Umezawa, Shiro Iwanaga, Takahiro Oda, Hajime Okita, Kensuke Kimura, Megumi Shimada, Mie Tanaka, Makoto Sano, Satoshi Ogawa, Jun ichi Hata

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Abstract

EAT/mcl-1 (EAT), a bcl-2-related immediate early gene, is up-regulated at an early stage of differentiation of human embryonal carcinoma cells. Recent studies have revealed that EAT inhibits apoptosis both in vitro and in vivo. In the present study, we demonstrated that the EAT gene was up-regulated in the early stage of rat myocardial infarction. This pattern of up-regulation was apparently different from that of another immediate early gene, c-fos. EAT, an anti-apoptotic molecule, was strongly up-regulated in the non-ischemic region. In contrast, the expression of c-fos was induced in both ischemic and non-ischemic regions, and was higher in the ischemic region. Apoptosis of cardiomyocytes is currently thought to significantly contribute to acute myocardial infarction. We detected cardiomyocyte apoptosis by gel electrophoresis of genomic DNA and in situ nick end labeling in the ischemic region, but not in the non-ischemic region. As an inhibitor of apoptosis, EAT may play a role in the protection of cardiomyocytes in the early stage of acute myocardial infarction. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)471-478
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume1472
Issue number3
DOIs
Publication statusPublished - 1999 Nov 16

Keywords

  • Apoptosis
  • Bcl-2
  • EAT/mcl-1
  • Ischemia
  • Myocardial infarction
  • c-Fos

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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    Matsushita, K., Umezawa, A., Iwanaga, S., Oda, T., Okita, H., Kimura, K., Shimada, M., Tanaka, M., Sano, M., Ogawa, S., & Hata, J. I. (1999). The EAT/mcl-1 gene, an inhibitor of apoptosis, is up-regulated in the early stage of acute myocardial infarction. Biochimica et Biophysica Acta - General Subjects, 1472(3), 471-478. https://doi.org/10.1016/S0304-4165(99)00149-X