The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture

Shinichi Maeno, Yasuo Niki, Hideo Matsumoto, Hideo Morioka, Taku Yatabe, Atsushi Funayama, Yoshiaki Toyama, Tetsushi Taguchi, Junzo Tanaka

Research output: Contribution to journalArticle

448 Citations (Scopus)

Abstract

Our research group aims to develop an osteochondral composite using type II collagen gel with hydroxyapatite (HAp) deposited on one side. Soaking gels in Ca2+ and phosphate solution is indispensable to HAp deposition, so relationships between cell behavior and Ca2+ concentration were examined in two- and three-dimensional cultures. The present results indicate that 2-4 mM Ca2+ is suitable for proliferation and survival of osteoblasts, whereas slightly higher concentrations (6-8 mM) favor osteoblast differentiation and matrix mineralization in both 2- and 3-dimensional cultures. Higher concentrations (>10mM) are cytotoxic. Purely from the perspective of calcium deposition, higher concentrations lead to increased accumulation of Ca2+. Culturing cells in phosphate-containing gel in media with Ca2+ also leads to time-dependent formation of HAp in the gel. Considering the viability of embedded cells, culturing scaffolds in media with Ca2+ concentrations around 5 mM is useful for both HAp deposition and osteoblast behavior.

Original languageEnglish
Pages (from-to)4847-4855
Number of pages9
JournalBiomaterials
Volume26
Issue number23
DOIs
Publication statusPublished - 2005 Aug

Keywords

  • Calcification
  • Medium calcium
  • Osteoblast
  • Tissue engineering
  • Type II collagen gel
  • Viability

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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  • Cite this

    Maeno, S., Niki, Y., Matsumoto, H., Morioka, H., Yatabe, T., Funayama, A., Toyama, Y., Taguchi, T., & Tanaka, J. (2005). The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials, 26(23), 4847-4855. https://doi.org/10.1016/j.biomaterials.2005.01.006