The effect of PTEN on proliferation and drug-, and radiosensitivity in malignant glioma cells

Nobuharu Inaba, Masaki Kimura, Kouki Fujioka, Keiichi Ikeda, Hiroko Somura, Kouhei Akiyoshi, Yuriko Inoue, Mayumi Nomura, Yuji Saito, Hidetsugu Saito, Yoshinobu Manome

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Deletions or mutations of the phosphatase and tensin homolog (PTEN) are frequently observed in malignant glioma and are responsible for progression of the disease. Since the molecule is a promising target for gene therapy, the effects of PTEN on glioma proliferation in combination with the anti-neoplastic agent, temozolomide, and ionizing radiation were investigated. Materials and Methods: An adenoviral vector encoding PTEN was used. After infection, changes in proliferation, the cell cycle, as well as drug- and radiosensitivity were investigated. Results: Expression of PTEN led to a 1.21-fold prolongation of the doubling time of the cells. It reduced G 1 and increased G2/M populations. Forced PTEN expression conferred sensitivity to temozolomide and/or ionizing radiation. Conclusion: In addition to counteracting cell proliferation, expression of PTEN presented advantages in the chemo- and radiosensitivity of glioma cells. Methods for up-regulation of PTEN may have a role in increasing the efficacy of current adjuvant therapies.

Original languageEnglish
Pages (from-to)1653-1658
Number of pages6
JournalAnticancer Research
Volume31
Issue number5
Publication statusPublished - 2011 May

    Fingerprint

Keywords

  • Cell cycle
  • Glioma
  • Ionizing radiation
  • PTEN
  • Radiosensitivity
  • Temozolomide

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Inaba, N., Kimura, M., Fujioka, K., Ikeda, K., Somura, H., Akiyoshi, K., Inoue, Y., Nomura, M., Saito, Y., Saito, H., & Manome, Y. (2011). The effect of PTEN on proliferation and drug-, and radiosensitivity in malignant glioma cells. Anticancer Research, 31(5), 1653-1658.