Abstract
Background: Deletions or mutations of the phosphatase and tensin homolog (PTEN) are frequently observed in malignant glioma and are responsible for progression of the disease. Since the molecule is a promising target for gene therapy, the effects of PTEN on glioma proliferation in combination with the anti-neoplastic agent, temozolomide, and ionizing radiation were investigated. Materials and Methods: An adenoviral vector encoding PTEN was used. After infection, changes in proliferation, the cell cycle, as well as drug- and radiosensitivity were investigated. Results: Expression of PTEN led to a 1.21-fold prolongation of the doubling time of the cells. It reduced G 1 and increased G2/M populations. Forced PTEN expression conferred sensitivity to temozolomide and/or ionizing radiation. Conclusion: In addition to counteracting cell proliferation, expression of PTEN presented advantages in the chemo- and radiosensitivity of glioma cells. Methods for up-regulation of PTEN may have a role in increasing the efficacy of current adjuvant therapies.
| Original language | English |
|---|---|
| Pages (from-to) | 1653-1658 |
| Number of pages | 6 |
| Journal | Anticancer Research |
| Volume | 31 |
| Issue number | 5 |
| Publication status | Published - 2011 May |
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Keywords
- Cell cycle
- Glioma
- Ionizing radiation
- PTEN
- Radiosensitivity
- Temozolomide
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
The effect of PTEN on proliferation and drug-, and radiosensitivity in malignant glioma cells. / Inaba, Nobuharu; Kimura, Masaki; Fujioka, Kouki; Ikeda, Keiichi; Somura, Hiroko; Akiyoshi, Kouhei; Inoue, Yuriko; Nomura, Mayumi; Saito, Yuji; Saito, Hidetsugu; Manome, Yoshinobu.
In: Anticancer Research, Vol. 31, No. 5, 05.2011, p. 1653-1658.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of PTEN on proliferation and drug-, and radiosensitivity in malignant glioma cells
AU - Inaba, Nobuharu
AU - Kimura, Masaki
AU - Fujioka, Kouki
AU - Ikeda, Keiichi
AU - Somura, Hiroko
AU - Akiyoshi, Kouhei
AU - Inoue, Yuriko
AU - Nomura, Mayumi
AU - Saito, Yuji
AU - Saito, Hidetsugu
AU - Manome, Yoshinobu
PY - 2011/5
Y1 - 2011/5
N2 - Background: Deletions or mutations of the phosphatase and tensin homolog (PTEN) are frequently observed in malignant glioma and are responsible for progression of the disease. Since the molecule is a promising target for gene therapy, the effects of PTEN on glioma proliferation in combination with the anti-neoplastic agent, temozolomide, and ionizing radiation were investigated. Materials and Methods: An adenoviral vector encoding PTEN was used. After infection, changes in proliferation, the cell cycle, as well as drug- and radiosensitivity were investigated. Results: Expression of PTEN led to a 1.21-fold prolongation of the doubling time of the cells. It reduced G 1 and increased G2/M populations. Forced PTEN expression conferred sensitivity to temozolomide and/or ionizing radiation. Conclusion: In addition to counteracting cell proliferation, expression of PTEN presented advantages in the chemo- and radiosensitivity of glioma cells. Methods for up-regulation of PTEN may have a role in increasing the efficacy of current adjuvant therapies.
AB - Background: Deletions or mutations of the phosphatase and tensin homolog (PTEN) are frequently observed in malignant glioma and are responsible for progression of the disease. Since the molecule is a promising target for gene therapy, the effects of PTEN on glioma proliferation in combination with the anti-neoplastic agent, temozolomide, and ionizing radiation were investigated. Materials and Methods: An adenoviral vector encoding PTEN was used. After infection, changes in proliferation, the cell cycle, as well as drug- and radiosensitivity were investigated. Results: Expression of PTEN led to a 1.21-fold prolongation of the doubling time of the cells. It reduced G 1 and increased G2/M populations. Forced PTEN expression conferred sensitivity to temozolomide and/or ionizing radiation. Conclusion: In addition to counteracting cell proliferation, expression of PTEN presented advantages in the chemo- and radiosensitivity of glioma cells. Methods for up-regulation of PTEN may have a role in increasing the efficacy of current adjuvant therapies.
KW - Cell cycle
KW - Glioma
KW - Ionizing radiation
KW - PTEN
KW - Radiosensitivity
KW - Temozolomide
UR - http://www.scopus.com/inward/record.url?scp=79958138485&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79958138485&partnerID=8YFLogxK
M3 - Article
C2 - 21617223
AN - SCOPUS:79958138485
VL - 31
SP - 1653
EP - 1658
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 5
ER -