Abstract
Background: Deletions or mutations of the phosphatase and tensin homolog (PTEN) are frequently observed in malignant glioma and are responsible for progression of the disease. Since the molecule is a promising target for gene therapy, the effects of PTEN on glioma proliferation in combination with the anti-neoplastic agent, temozolomide, and ionizing radiation were investigated. Materials and Methods: An adenoviral vector encoding PTEN was used. After infection, changes in proliferation, the cell cycle, as well as drug- and radiosensitivity were investigated. Results: Expression of PTEN led to a 1.21-fold prolongation of the doubling time of the cells. It reduced G 1 and increased G2/M populations. Forced PTEN expression conferred sensitivity to temozolomide and/or ionizing radiation. Conclusion: In addition to counteracting cell proliferation, expression of PTEN presented advantages in the chemo- and radiosensitivity of glioma cells. Methods for up-regulation of PTEN may have a role in increasing the efficacy of current adjuvant therapies.
Original language | English |
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Pages (from-to) | 1653-1658 |
Number of pages | 6 |
Journal | Anticancer research |
Volume | 31 |
Issue number | 5 |
Publication status | Published - 2011 May |
Keywords
- Cell cycle
- Glioma
- Ionizing radiation
- PTEN
- Radiosensitivity
- Temozolomide
ASJC Scopus subject areas
- Oncology
- Cancer Research