The effect of temperature on the morphology of fibroblasts in a collagen gel in nutrient-free medium

Hiroshi Yamada, Shinji Nobuhara, Shogo Miyata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The effect of temperature on the morphology of fibroblasts in a collagen gel (living dermal model (Toyobo, Japan)) was investigated to And an efficient way to delay the occurrence of pressure ulcers in malnourished patients. Cells were observed microscopically after culture for 0, 3, and 6 h at 37 or 25°C in Dulbecco's phosphate-buffered saline (DPBS) in an incubator under 5% CO 2/95% air and 100% humidity. We measured the aspect ratio of cells located 10 μm below the gel surface after 0, 3, and 6 h under each condition. The mean±SD aspect ratio was 16.9±5.1 and 7.1±4.0 (n = 16) at 0 and 6 h, respectively, for cells incubated at 37°C and 13.8±4.1 and 14.5±6.1 (n = 16) at 0 and 6 h, for cells incubated at 25°C. This indicates that cell viability can be maintained by lowering the temperature when the supply of nutrients is limited.

Original languageEnglish
Title of host publication5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
Pages539-540
Number of pages2
DOIs
Publication statusPublished - 2008
Externally publishedYes
Event5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008 - Shenzhen, China
Duration: 2008 May 302008 May 31

Other

Other5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
CountryChina
CityShenzhen
Period08/5/3008/5/31

Fingerprint

Fibroblasts
Collagen
Nutrients
Aspect ratio
Gels
Food
Temperature
Atmospheric humidity
Phosphates
Cells
Incubators
Pressure Ulcer
Carbon Monoxide
Air
Humidity
Cell Survival
Japan
Skin

ASJC Scopus subject areas

  • Computer Science(all)
  • Biomedical Engineering
  • Health Informatics

Cite this

Yamada, H., Nobuhara, S., & Miyata, S. (2008). The effect of temperature on the morphology of fibroblasts in a collagen gel in nutrient-free medium. In 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008 (pp. 539-540). [4570592] https://doi.org/10.1109/ITAB.2008.4570592

The effect of temperature on the morphology of fibroblasts in a collagen gel in nutrient-free medium. / Yamada, Hiroshi; Nobuhara, Shinji; Miyata, Shogo.

5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008. 2008. p. 539-540 4570592.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yamada, H, Nobuhara, S & Miyata, S 2008, The effect of temperature on the morphology of fibroblasts in a collagen gel in nutrient-free medium. in 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008., 4570592, pp. 539-540, 5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008, Shenzhen, China, 08/5/30. https://doi.org/10.1109/ITAB.2008.4570592
Yamada H, Nobuhara S, Miyata S. The effect of temperature on the morphology of fibroblasts in a collagen gel in nutrient-free medium. In 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008. 2008. p. 539-540. 4570592 https://doi.org/10.1109/ITAB.2008.4570592
Yamada, Hiroshi ; Nobuhara, Shinji ; Miyata, Shogo. / The effect of temperature on the morphology of fibroblasts in a collagen gel in nutrient-free medium. 5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008. 2008. pp. 539-540
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