The effect of the microstructure of β-tricalcium phosphate on the metabolism of subsequently formed bone tissue

Takatoshi Okuda, Koji Ioku, Ikuho Yonezawa, Hideyuki Minagi, Giichiro Kawachi, Yoshinori Gonda, Hisashi Murayama, Yasuaki Shibata, Soichiro Minami, Shimeru Kamihira, Hisashi Kurosawa, Tohru Ikeda

Research output: Contribution to journalArticlepeer-review

131 Citations (Scopus)


The response of bone cells to a newly developed porous β-tricalcium phosphate composed of rod-shaped particles (RSβ-TCP), β-TCP composed of conventional non-rod-shaped particles (Cβ-TCP), and hydroxyapatite (HA) was analyzed using in vivo implantation and in vitro osteoclastogenesis systems. Implantation of the materials into the rabbit femur showed that RSβ-TCP and Cβ-TCP were bioresorbable, but HA was not. Up to 12 weeks after the implantation, bioresorption of RSβ-TCP and Cβ-TCP accompanied by the formation of new bone occurred satisfactorily. At 24 weeks post-implantation, most of the RSβ-TCP had been absorbed, and active osteogenesis was preserved in the region. However, in the specimens implanted with Cβ-TCP, the amount of not only the implanted Cβ-TCP but also the newly formed bone tissue decreased, and bone marrow dominated the region. The implanted HA was unbioresorbable throughout the experimental period. When osteoclasts were generated on RSβ-TCP, Cβ-TCP, or HA disks, apparent resorption lacunae were formed on the RSβ-TCP and Cβ-TCP, but not HA disks. Quantitation of the calcium concentration in the culture media showed an earlier and more constant release of calcium from RSβ-TCP than Cβ-TCP. These results showed that the microstructure of β-TCP affects the activity of bone cells and subsequent bone replacement.

Original languageEnglish
Pages (from-to)2612-2621
Number of pages10
Issue number16
Publication statusPublished - 2007 Jun
Externally publishedYes


  • Biodegradation
  • Bone graft
  • Coculture
  • Osteoblast
  • Osteoclast

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


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