The effects of age and abnormal sperm count on the nondisjunction of spermatozoa

H. Asada, Kou Sueoka, T. Hashiba, M. Kuroshima, N. Kobayashi, Y. Yoshimura

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Purpose: The effect of paternal age on the nondisjunction of sex chromosomes is controversial. Also, the prevalence of chromosomal anomalies in infertile patients is controversial, it has been reported that the sex chromosomal aneuploidy rate following treatment with intracytoplasmic sperm injection (ICSI) is higher than in naturally conceived pregnancies. We investigated the influence of paternal age and oligozoospermia on the nondisjunction of spermatozoa. Methods: We determined the rate of aneuploidy for gonosomes and autosomes, using two-color fluorescence in situ hybridization (FISH) of the X and Y chromosomes and chromosomes 12 and 18 in 10 donors under 25 years of age who had a normal sperm count (≥20 X 106/ml), 10 donors over the age of 39 years with idiopathic infertility and normozoospermia (≥20 X 106/ml), and 5 oligozoospermic donors (<20 X 106/ml). Results: There was no obvious relationship between increasing age and autosomal disomy (disomy 12 and disomy 18). Neither autosomal disomy nor diploidy was increased in any group. The frequency of X-, Y-, XX-, and YY- bearing sperm did not differ significantly among groups, but the frequency of XY-bearing sperm was significantly higher in the older infertile group than in the control donors. Conclusions: The incidence of nondisjunction of paternal sex chromosome in meiosis I was higher in older men with idiopathic infertility. The present results suggest that the risk of producing XXY fetuses is higher among men >39 years of age with idiopathic infertility.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalJournal of Assisted Reproduction and Genetics
Volume17
Issue number1
DOIs
Publication statusPublished - 2000

Fingerprint

Sperm Count
Paternal Age
Spermatozoa
Sex Chromosomes
Tissue Donors
Aneuploidy
Infertility
Chromosomes, Human, Pair 18
Oligospermia
Chromosomes, Human, Pair 12
Intracytoplasmic Sperm Injections
Y Chromosome
X Chromosome
Fluorescence In Situ Hybridization
Color
Pregnancy
Therapeutics

Keywords

  • Nondisjunction
  • Paternal age
  • Sex chromosome
  • Spermatozoa

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics
  • Reproductive Medicine

Cite this

The effects of age and abnormal sperm count on the nondisjunction of spermatozoa. / Asada, H.; Sueoka, Kou; Hashiba, T.; Kuroshima, M.; Kobayashi, N.; Yoshimura, Y.

In: Journal of Assisted Reproduction and Genetics, Vol. 17, No. 1, 2000, p. 51-59.

Research output: Contribution to journalArticle

Asada, H. ; Sueoka, Kou ; Hashiba, T. ; Kuroshima, M. ; Kobayashi, N. ; Yoshimura, Y. / The effects of age and abnormal sperm count on the nondisjunction of spermatozoa. In: Journal of Assisted Reproduction and Genetics. 2000 ; Vol. 17, No. 1. pp. 51-59.
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