The effects of coffee on conjugation reactions in human colon carcinoma cells

Shigeaki Okamura, Keiko Suzuki, Masayo Yanase, Masumi Koizumi, Hiroomi Tamura

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We examined the effect of coffee on conjugation reactions in the human colon carcinoma cell line, Caco-2. After supplementing Caco-2 cultures with both 1-naphthol (200 μM) and various concentrations of coffee, the accumulation of 1-naphthyl sulfate and glucuronide in the growth medium was determined by analytical HPLC over a 24-h period. A strong reduction in sulfo-conjugation (<50% of the control value) was observed in cells treated with coffee (IC50=4.3%), but no effect on glucuronic acid conjugation (glucuronidation) was observed. Coffee was also found to inhibit sulfotransferase (SULT) activity towards 1-naphthol in vitro to a similar extent (IC50=5.1%) as in intact Caco-2 cells, but exhibited no effect upon UDP-glucuronosyl transferase (UGT) activity in vitro. PCR analyses showed no significant changes in the expression of either SULT genes (SULT1A1 and SULT1A3) or UGT genes (UGT1A1 and UGT1A6) following treatment with coffee solutions of up to 5% in concentration. These results suggest that the consumption of coffee can modify sulfo-conjugation reactions within intestinal epithelial cells, which may possibly affect the bioavailability of therapeutic drugs and the toxicity of environmental chemicals.

Original languageEnglish
Pages (from-to)271-274
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number2
DOIs
Publication statusPublished - 2005 Feb

Fingerprint

Coffee
Colon
Carcinoma
Sulfotransferases
Uridine Diphosphate
Transferases
Inhibitory Concentration 50
Glucuronic Acid
Caco-2 Cells
Drug-Related Side Effects and Adverse Reactions
Biological Availability
Genes
Epithelial Cells
High Pressure Liquid Chromatography
Cell Line
Polymerase Chain Reaction
Growth

Keywords

  • Caco-2
  • Coffee
  • Conjugation

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

The effects of coffee on conjugation reactions in human colon carcinoma cells. / Okamura, Shigeaki; Suzuki, Keiko; Yanase, Masayo; Koizumi, Masumi; Tamura, Hiroomi.

In: Biological and Pharmaceutical Bulletin, Vol. 28, No. 2, 02.2005, p. 271-274.

Research output: Contribution to journalArticle

Okamura, Shigeaki ; Suzuki, Keiko ; Yanase, Masayo ; Koizumi, Masumi ; Tamura, Hiroomi. / The effects of coffee on conjugation reactions in human colon carcinoma cells. In: Biological and Pharmaceutical Bulletin. 2005 ; Vol. 28, No. 2. pp. 271-274.
@article{bd40f8cd183d4124aa84d5e66813e3df,
title = "The effects of coffee on conjugation reactions in human colon carcinoma cells",
abstract = "We examined the effect of coffee on conjugation reactions in the human colon carcinoma cell line, Caco-2. After supplementing Caco-2 cultures with both 1-naphthol (200 μM) and various concentrations of coffee, the accumulation of 1-naphthyl sulfate and glucuronide in the growth medium was determined by analytical HPLC over a 24-h period. A strong reduction in sulfo-conjugation (<50{\%} of the control value) was observed in cells treated with coffee (IC50=4.3{\%}), but no effect on glucuronic acid conjugation (glucuronidation) was observed. Coffee was also found to inhibit sulfotransferase (SULT) activity towards 1-naphthol in vitro to a similar extent (IC50=5.1{\%}) as in intact Caco-2 cells, but exhibited no effect upon UDP-glucuronosyl transferase (UGT) activity in vitro. PCR analyses showed no significant changes in the expression of either SULT genes (SULT1A1 and SULT1A3) or UGT genes (UGT1A1 and UGT1A6) following treatment with coffee solutions of up to 5{\%} in concentration. These results suggest that the consumption of coffee can modify sulfo-conjugation reactions within intestinal epithelial cells, which may possibly affect the bioavailability of therapeutic drugs and the toxicity of environmental chemicals.",
keywords = "Caco-2, Coffee, Conjugation",
author = "Shigeaki Okamura and Keiko Suzuki and Masayo Yanase and Masumi Koizumi and Hiroomi Tamura",
year = "2005",
month = "2",
doi = "10.1248/bpb.28.271",
language = "English",
volume = "28",
pages = "271--274",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "2",

}

TY - JOUR

T1 - The effects of coffee on conjugation reactions in human colon carcinoma cells

AU - Okamura, Shigeaki

AU - Suzuki, Keiko

AU - Yanase, Masayo

AU - Koizumi, Masumi

AU - Tamura, Hiroomi

PY - 2005/2

Y1 - 2005/2

N2 - We examined the effect of coffee on conjugation reactions in the human colon carcinoma cell line, Caco-2. After supplementing Caco-2 cultures with both 1-naphthol (200 μM) and various concentrations of coffee, the accumulation of 1-naphthyl sulfate and glucuronide in the growth medium was determined by analytical HPLC over a 24-h period. A strong reduction in sulfo-conjugation (<50% of the control value) was observed in cells treated with coffee (IC50=4.3%), but no effect on glucuronic acid conjugation (glucuronidation) was observed. Coffee was also found to inhibit sulfotransferase (SULT) activity towards 1-naphthol in vitro to a similar extent (IC50=5.1%) as in intact Caco-2 cells, but exhibited no effect upon UDP-glucuronosyl transferase (UGT) activity in vitro. PCR analyses showed no significant changes in the expression of either SULT genes (SULT1A1 and SULT1A3) or UGT genes (UGT1A1 and UGT1A6) following treatment with coffee solutions of up to 5% in concentration. These results suggest that the consumption of coffee can modify sulfo-conjugation reactions within intestinal epithelial cells, which may possibly affect the bioavailability of therapeutic drugs and the toxicity of environmental chemicals.

AB - We examined the effect of coffee on conjugation reactions in the human colon carcinoma cell line, Caco-2. After supplementing Caco-2 cultures with both 1-naphthol (200 μM) and various concentrations of coffee, the accumulation of 1-naphthyl sulfate and glucuronide in the growth medium was determined by analytical HPLC over a 24-h period. A strong reduction in sulfo-conjugation (<50% of the control value) was observed in cells treated with coffee (IC50=4.3%), but no effect on glucuronic acid conjugation (glucuronidation) was observed. Coffee was also found to inhibit sulfotransferase (SULT) activity towards 1-naphthol in vitro to a similar extent (IC50=5.1%) as in intact Caco-2 cells, but exhibited no effect upon UDP-glucuronosyl transferase (UGT) activity in vitro. PCR analyses showed no significant changes in the expression of either SULT genes (SULT1A1 and SULT1A3) or UGT genes (UGT1A1 and UGT1A6) following treatment with coffee solutions of up to 5% in concentration. These results suggest that the consumption of coffee can modify sulfo-conjugation reactions within intestinal epithelial cells, which may possibly affect the bioavailability of therapeutic drugs and the toxicity of environmental chemicals.

KW - Caco-2

KW - Coffee

KW - Conjugation

UR - http://www.scopus.com/inward/record.url?scp=19444385420&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19444385420&partnerID=8YFLogxK

U2 - 10.1248/bpb.28.271

DO - 10.1248/bpb.28.271

M3 - Article

VL - 28

SP - 271

EP - 274

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 2

ER -