The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function

Yosuke Iizumi, Hiroshi Sagara, Yasuaki Kabe, Motoki Azuma, Kanako Kume, Michinaga Ogawa, Takeshi Nagai, Peter G. Gillespie, Chihiro Sasakawa, Hiroshi Handa

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Enteropathogenic Escherichia coli (EPEC) destroys intestinal microvilli and suppresses phagocytosis by injecting effectors into infected cells through a type III secretion system (TTSS). EspB, a component of the TTSS, is also injected into the cytoplasm of host cells. However, the physiological functions of EspB within the host cell cytoplasm remain unclear. We show that EspB binds to myosins, which are a superfamily of proteins that interact with actin filaments and mediate essential cellular processes, including microvillus formation and phagocytosis. EspB inhibits the interaction of myosins with actin, and an EspB mutant that lacks the myosin-binding region maintained its TTSS function but could not induce microvillus effacing or suppress phagocytosis. Moreover, the myosin-binding region of EspB is essential for Citrobacter rodentium, an EPEC-related murine pathogen, to efficiently infect mice. These results suggest that EspB inhibits myosin functions and thereby facilitates efficient infection by EPEC.

Original languageEnglish
Pages (from-to)383-392
Number of pages10
JournalCell Host and Microbe
Volume2
Issue number6
DOIs
Publication statusPublished - 2007 Dec 13
Externally publishedYes

Fingerprint

Enteropathogenic Escherichia coli
Myosins
Microvilli
Phagocytosis
Citrobacter rodentium
Cytoplasm
Actin Cytoskeleton
Actins
Infection
Type III Secretion Systems
Proteins

Keywords

  • CELLBIO
  • MICROBIO

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function. / Iizumi, Yosuke; Sagara, Hiroshi; Kabe, Yasuaki; Azuma, Motoki; Kume, Kanako; Ogawa, Michinaga; Nagai, Takeshi; Gillespie, Peter G.; Sasakawa, Chihiro; Handa, Hiroshi.

In: Cell Host and Microbe, Vol. 2, No. 6, 13.12.2007, p. 383-392.

Research output: Contribution to journalArticle

Iizumi, Y, Sagara, H, Kabe, Y, Azuma, M, Kume, K, Ogawa, M, Nagai, T, Gillespie, PG, Sasakawa, C & Handa, H 2007, 'The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function', Cell Host and Microbe, vol. 2, no. 6, pp. 383-392. https://doi.org/10.1016/j.chom.2007.09.012
Iizumi, Yosuke ; Sagara, Hiroshi ; Kabe, Yasuaki ; Azuma, Motoki ; Kume, Kanako ; Ogawa, Michinaga ; Nagai, Takeshi ; Gillespie, Peter G. ; Sasakawa, Chihiro ; Handa, Hiroshi. / The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function. In: Cell Host and Microbe. 2007 ; Vol. 2, No. 6. pp. 383-392.
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