The evaluation of the treatment response in obstructive meibomian gland disease by in vivo laser confocal microscopy

Yukihiro Matsumoto, Yuta Shigeno, Enrique Adan Sato, Osama M A Ibrahim, Megumi Saiki, Kazuno Negishi, Yoko Ogawa, Murat Dogru, Kazuo Tsubota

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Purpose: To evaluate the status of periglandular inflammation, ocular surface and tear function alterations in patients with obstructive meibomian gland disease (OMGD) by in vivo confocal microscopy before and after anti-inflammatory treatment, and to compare the results with patients receiving only topical non-preserved artificial tears and sodium hyaluronate eye drops without anti-inflammatory agents. Methods: Thirty-two eyes of 16 OMGD patients receiving anti-inflammatory treatment (treatment group) and 22 eyes of 11 OMGD patients receiving only topical non-preserved artificial tears and sodium hyaluronate eye drops (control group) were recruited in this prospective study. All subjects underwent slit-lamp examinations, tear film break-up time (BUT) measurements, fluorescein and Rose-Bengal stainings, Schirmer test I without anesthesia, transillumination of the lids (meibography), and in vivo laser confocal microscopy of the lids (HRTII-RCM). Results: The mean BUT, fluorescein staining scores, and inflammatory cell densities observed by in vivo confocal microscopy improved significantly in the group receiving anti-inflammatory treatment (p<0.05), whereas no significant alterations of these parameters were observed in the group not receiving anti-inflammatory agents (p>0.05). Conclusions: In vivo confocal microscopy was able to effectively demonstrate the treatment responses in patients with OMGD. Inflammatory cell density calculation seems to be a promising new parameter of in vivo confocal microscopy in the evaluation of treatment responses.

Original languageEnglish
Pages (from-to)821-829
Number of pages9
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume247
Issue number6
DOIs
Publication statusPublished - 2009 Jun

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Meibomian Glands
Confocal Microscopy
Anti-Inflammatory Agents
Ophthalmic Solutions
Hyaluronic Acid
Fluorescein
Tears
Therapeutics
Cell Count
Transillumination
Staining and Labeling
Rose Bengal
Intravital Microscopy
Anesthesia
Prospective Studies
Inflammation
Control Groups

Keywords

  • Confocal microscopy
  • Inflammation
  • Meibomian gland dysfunction

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

The evaluation of the treatment response in obstructive meibomian gland disease by in vivo laser confocal microscopy. / Matsumoto, Yukihiro; Shigeno, Yuta; Sato, Enrique Adan; Ibrahim, Osama M A; Saiki, Megumi; Negishi, Kazuno; Ogawa, Yoko; Dogru, Murat; Tsubota, Kazuo.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 247, No. 6, 06.2009, p. 821-829.

Research output: Contribution to journalArticle

Matsumoto, Yukihiro ; Shigeno, Yuta ; Sato, Enrique Adan ; Ibrahim, Osama M A ; Saiki, Megumi ; Negishi, Kazuno ; Ogawa, Yoko ; Dogru, Murat ; Tsubota, Kazuo. / The evaluation of the treatment response in obstructive meibomian gland disease by in vivo laser confocal microscopy. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 2009 ; Vol. 247, No. 6. pp. 821-829.
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