The genomic structure and promoter region of the human Parkin gene

S. Asakawa, K. I. Tsunematsu, A. Takayanagi, T. Sasaki, A. Shimizu, A. Shintani, K. Kawasaki, A. J. Mungall, S. Beck, S. Minoshima, N. Shimizu

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Parkin has been identified as a causative gene of the autosomal recessive juvenile parkinsonism (AR-JP). In this study, we determined the genomic structure of the Parkin gene and identified a core promoter region based on the DNA sequence of 1.4 Mb. The 5′-flanking region contained no apparent TATA or CAAT box elements but several putative cis-elements for various transcription factors. The GC- and CpG-rich regions were observed not only in the 5′-flanking sequence but also in the 5′-part of the first intron of Parkin. We identified an exact starting point of Parkin transcription. A core promoter region was determined by transfecting a series of deletion constructs with a dual luciferase reporter system into human neuroblastoma cells. Furthermore, we located a neighboring novel gene in a head-to-head direction with Parkin with only a 198-bp interval.

Original languageEnglish
Pages (from-to)863-868
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume286
Issue number5
DOIs
Publication statusPublished - 2001 Jan 1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Asakawa, S., Tsunematsu, K. I., Takayanagi, A., Sasaki, T., Shimizu, A., Shintani, A., Kawasaki, K., Mungall, A. J., Beck, S., Minoshima, S., & Shimizu, N. (2001). The genomic structure and promoter region of the human Parkin gene. Biochemical and Biophysical Research Communications, 286(5), 863-868. https://doi.org/10.1006/bbrc.2001.5490