The glutamate receptor agonist, AMPA, induces acetylcholine release in guinea pig cochlea; a microdialysis study

Noriyuki Hoya, Kaoru Ogawa, Yasuhiro Inoue, Yoichiro Takiguchi, Jin Kanzaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Acetylcholine (Ach) has been considered a major neurotransmitter in the inner ear efferent nerve endings. A bioassay analysis has shown that the electrical stimulation of the crossed olivocochlear bundle increased the Ach-like activity in the perilymph. Applying in vivo microdialysis techniques and high-performance liquid chromatography to the perilymph, the change of Ach level was thus measured before and after α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA), a glutamate receptor agonist, was added to the perfusate. Ach was only detectable when the perfusate contained an acetylcholinesterase inhibitor. The level of Ach increased 2-3-fold immediately after AMPA was administered. Our data suggest that the afferent stimulation, such as the administration of AMPA, may therefore induce the release of Ach from the efferent nerve endings.

Original languageEnglish
Pages (from-to)206-208
Number of pages3
JournalNeuroscience Letters
Volume311
Issue number3
DOIs
Publication statusPublished - 2001 Oct 5

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Excitatory Amino Acid Agonists
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Cochlea
Microdialysis
Glutamate Receptors
Acetylcholine
Guinea Pigs
Perilymph
Nerve Endings
Isoxazoles
Cholinesterase Inhibitors
Inner Ear
Biological Assay
Electric Stimulation
Neurotransmitter Agents
High Pressure Liquid Chromatography

Keywords

  • α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid
  • 6-cyano-7-nitroquinoxaline-2,3-dione
  • Acetylcholine
  • Cochlea
  • Efferent
  • Guinea pig
  • Microdialysis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The glutamate receptor agonist, AMPA, induces acetylcholine release in guinea pig cochlea; a microdialysis study. / Hoya, Noriyuki; Ogawa, Kaoru; Inoue, Yasuhiro; Takiguchi, Yoichiro; Kanzaki, Jin.

In: Neuroscience Letters, Vol. 311, No. 3, 05.10.2001, p. 206-208.

Research output: Contribution to journalArticle

Hoya, Noriyuki ; Ogawa, Kaoru ; Inoue, Yasuhiro ; Takiguchi, Yoichiro ; Kanzaki, Jin. / The glutamate receptor agonist, AMPA, induces acetylcholine release in guinea pig cochlea; a microdialysis study. In: Neuroscience Letters. 2001 ; Vol. 311, No. 3. pp. 206-208.
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