The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster

Takehide Murata, Hideyuki Nagaso, Shin ichi Kashiwabara, Tadashi Baba, Hideyuki Okano, Kazunari K. Yokoyama

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The hiiragi (hrg) gene plays a key role in the development of the wing margin in Drosophila melanogaster. A mutation in the hrg gene resulted in a decrease in the level of the hrg transcript and was associated with a notched wing phenotype. We report here that the hrg gene encodes a poly(A) polymerase(PAP). The bovine cDNA for PAP type II reversed the phenotype due to mutation of the hrg gene, suggesting that hrg might encode a functional homolog of PAP. A mutation that reduced the enzymatic activity of Hrg failed to reverse the phenotype of hrg mutants, suggesting that the enzymatic activity of Hrg was required to rescue the wing phenotype. The levels of expression of wingless and cut at the presumptive wing margins were reduced in the late third-instar larvae of hrg mutants. These results suggest that the product of hrg is required for the normal expression of a series of genes in this region. Our results provide the first evidence that a PAP in Drosophila plays a key role in the early development of the wing margin, acting to regulate the specific expression of a series of genes via, perhaps, control of the processing of the 3′ ends of transcripts.

Original languageEnglish
Pages (from-to)137-147
Number of pages11
JournalDevelopmental Biology
Volume233
Issue number1
DOIs
Publication statusPublished - 2001 May 1
Externally publishedYes

Fingerprint

Polynucleotide Adenylyltransferase
Drosophila melanogaster
Genes
Phenotype
Mutation
Drosophila
Larva
Complementary DNA

Keywords

  • Cut
  • Drosophila
  • Notch
  • Poly(A) polymerase
  • Wing development
  • Wingless

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster. / Murata, Takehide; Nagaso, Hideyuki; Kashiwabara, Shin ichi; Baba, Tadashi; Okano, Hideyuki; Yokoyama, Kazunari K.

In: Developmental Biology, Vol. 233, No. 1, 01.05.2001, p. 137-147.

Research output: Contribution to journalArticle

Murata, Takehide ; Nagaso, Hideyuki ; Kashiwabara, Shin ichi ; Baba, Tadashi ; Okano, Hideyuki ; Yokoyama, Kazunari K. / The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster. In: Developmental Biology. 2001 ; Vol. 233, No. 1. pp. 137-147.
@article{242db273ba1c481db5ce13ea3e075ea7,
title = "The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster",
abstract = "The hiiragi (hrg) gene plays a key role in the development of the wing margin in Drosophila melanogaster. A mutation in the hrg gene resulted in a decrease in the level of the hrg transcript and was associated with a notched wing phenotype. We report here that the hrg gene encodes a poly(A) polymerase(PAP). The bovine cDNA for PAP type II reversed the phenotype due to mutation of the hrg gene, suggesting that hrg might encode a functional homolog of PAP. A mutation that reduced the enzymatic activity of Hrg failed to reverse the phenotype of hrg mutants, suggesting that the enzymatic activity of Hrg was required to rescue the wing phenotype. The levels of expression of wingless and cut at the presumptive wing margins were reduced in the late third-instar larvae of hrg mutants. These results suggest that the product of hrg is required for the normal expression of a series of genes in this region. Our results provide the first evidence that a PAP in Drosophila plays a key role in the early development of the wing margin, acting to regulate the specific expression of a series of genes via, perhaps, control of the processing of the 3′ ends of transcripts.",
keywords = "Cut, Drosophila, Notch, Poly(A) polymerase, Wing development, Wingless",
author = "Takehide Murata and Hideyuki Nagaso and Kashiwabara, {Shin ichi} and Tadashi Baba and Hideyuki Okano and Yokoyama, {Kazunari K.}",
year = "2001",
month = "5",
day = "1",
doi = "10.1006/dbio.2001.0205",
language = "English",
volume = "233",
pages = "137--147",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster

AU - Murata, Takehide

AU - Nagaso, Hideyuki

AU - Kashiwabara, Shin ichi

AU - Baba, Tadashi

AU - Okano, Hideyuki

AU - Yokoyama, Kazunari K.

PY - 2001/5/1

Y1 - 2001/5/1

N2 - The hiiragi (hrg) gene plays a key role in the development of the wing margin in Drosophila melanogaster. A mutation in the hrg gene resulted in a decrease in the level of the hrg transcript and was associated with a notched wing phenotype. We report here that the hrg gene encodes a poly(A) polymerase(PAP). The bovine cDNA for PAP type II reversed the phenotype due to mutation of the hrg gene, suggesting that hrg might encode a functional homolog of PAP. A mutation that reduced the enzymatic activity of Hrg failed to reverse the phenotype of hrg mutants, suggesting that the enzymatic activity of Hrg was required to rescue the wing phenotype. The levels of expression of wingless and cut at the presumptive wing margins were reduced in the late third-instar larvae of hrg mutants. These results suggest that the product of hrg is required for the normal expression of a series of genes in this region. Our results provide the first evidence that a PAP in Drosophila plays a key role in the early development of the wing margin, acting to regulate the specific expression of a series of genes via, perhaps, control of the processing of the 3′ ends of transcripts.

AB - The hiiragi (hrg) gene plays a key role in the development of the wing margin in Drosophila melanogaster. A mutation in the hrg gene resulted in a decrease in the level of the hrg transcript and was associated with a notched wing phenotype. We report here that the hrg gene encodes a poly(A) polymerase(PAP). The bovine cDNA for PAP type II reversed the phenotype due to mutation of the hrg gene, suggesting that hrg might encode a functional homolog of PAP. A mutation that reduced the enzymatic activity of Hrg failed to reverse the phenotype of hrg mutants, suggesting that the enzymatic activity of Hrg was required to rescue the wing phenotype. The levels of expression of wingless and cut at the presumptive wing margins were reduced in the late third-instar larvae of hrg mutants. These results suggest that the product of hrg is required for the normal expression of a series of genes in this region. Our results provide the first evidence that a PAP in Drosophila plays a key role in the early development of the wing margin, acting to regulate the specific expression of a series of genes via, perhaps, control of the processing of the 3′ ends of transcripts.

KW - Cut

KW - Drosophila

KW - Notch

KW - Poly(A) polymerase

KW - Wing development

KW - Wingless

UR - http://www.scopus.com/inward/record.url?scp=0035339120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035339120&partnerID=8YFLogxK

U2 - 10.1006/dbio.2001.0205

DO - 10.1006/dbio.2001.0205

M3 - Article

C2 - 11319863

AN - SCOPUS:0035339120

VL - 233

SP - 137

EP - 147

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -