The Hsp70 homolog gene, Hsc70t, is expressed under translational control during mouse spermiogenesis

Naoki Tsunekawa, Midori Matsumoto, Shigenobu Tone, Takao Nishida, Hirokazu Fujimoto

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Hsc70t is a member of the Hsp70 family of genes and is constitutively expressed after meiosis in mouse spermatogenesis. Immunohistochemistry and in situ hybridization techniques were used to examine the precise localization of the Hsc70t product during the various stages of spermatogenesis. A rabbit antiserum raised against the mouse Hsc70t-lacZ fusion protein detected the Hsc70t protein in the late spermatid-enriched fraction after two-dimensional Western blot analyses. On histological sections, the protein appears in the cytoplasm of spermatids as they progress from step 9 to the final step of spermatogenesis. An antisense RNA probe generated from the 3' untranslated region of Hsc70t cDNA detected Hsc70t mRNA in late round spermatids from step 7 onward with the signal disappearing in spermatids at step 15. Thus, Hsc70t mRNA first appears after meiosis in haploid cells but is not translated effectively until these cells progress to the transcriptionally inactive stage which coincides with chromatin condensation. These results establish that the synthesis of Hsc70t protein is under strict translational control.

Original languageEnglish
Pages (from-to)383-391
Number of pages9
JournalMolecular Reproduction and Development
Volume52
Issue number4
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Spermatids
Spermatogenesis
Meiosis
Genes
Proteins
RNA Probes
Antisense RNA
Messenger RNA
Haploidy
3' Untranslated Regions
Chromatin
In Situ Hybridization
Immune Sera
Cytoplasm
Complementary DNA
Western Blotting
Immunohistochemistry
Rabbits

Keywords

  • Gene expression
  • Immunohistochemistry
  • In situ hybridization
  • Spermatid
  • Translational control

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

The Hsp70 homolog gene, Hsc70t, is expressed under translational control during mouse spermiogenesis. / Tsunekawa, Naoki; Matsumoto, Midori; Tone, Shigenobu; Nishida, Takao; Fujimoto, Hirokazu.

In: Molecular Reproduction and Development, Vol. 52, No. 4, 1999, p. 383-391.

Research output: Contribution to journalArticle

Tsunekawa, Naoki ; Matsumoto, Midori ; Tone, Shigenobu ; Nishida, Takao ; Fujimoto, Hirokazu. / The Hsp70 homolog gene, Hsc70t, is expressed under translational control during mouse spermiogenesis. In: Molecular Reproduction and Development. 1999 ; Vol. 52, No. 4. pp. 383-391.
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