The identification of D-tryptophan as a bioactive substance for postembryonic ovarian development in the planarian Dugesia ryukyuensis

Kazuya Kobayashi, Takanobu Maezawa, Hiroyuki Tanaka, Hiroyuki Onuki, Yurie Horiguchi, Hiroshi Hirota, Tetsuo Ishida, Kihachiro Horiike, Yasutoshi Agata, Manabu Aoki, Motonori Hoshi, Midori Matsumoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Many metazoans start germ cell development during embryogenesis, while some metazoans possessing pluripotent stem cells undergo postembryonic germ cell development. The latter reproduce asexually but develop germ cells from pluripotent stem cells or dormant primordial germ cells when they reproduce sexually. Sexual induction of the planarian Dugesia ryukyuensis is an important model for postembryonic germ cell development. In this experimental system, hermaphroditic reproductive organs are differentiated in presumptive gonadal regions by the administration of a crude extract from sexual planarians to asexual ones. However, the substances involved in the first event during postembryonic germ cell development, i.e., ovarian development, remain unknown. Here, we aimed to identify a bioactive compound associated with postembryonic ovarian development. Bioassay-guided fractionation identified Ê-tryptophan (Trp) on the basis of electrospray ionization-mass spectrometry, circular dichroism, and nuclear magnetic resonance spectroscopy. Originally masked by a large amount of L-Trp, D-Trp was detected by reverse-phase high-performance liquid chromatography. The ovary-inducing activity of D-Trp was 500 times more potent than that of L-Trp. This is the first report describing a role for an intrinsic D-amino acid in postembryonic germ cell development. Our findings provide a novel insight into the mechanisms of germ cell development regulated by low-molecular weight bioactive compounds.

Original languageEnglish
Article number45175
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 2017 Mar 24

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Planarians
Germ Cells
Tryptophan
Pluripotent Stem Cells
Electrospray Ionization Mass Spectrometry
Reverse-Phase Chromatography
Circular Dichroism
Complex Mixtures
Biological Assay
Embryonic Development
Ovary
Magnetic Resonance Spectroscopy
Molecular Weight
High Pressure Liquid Chromatography
Amino Acids

ASJC Scopus subject areas

  • General

Cite this

The identification of D-tryptophan as a bioactive substance for postembryonic ovarian development in the planarian Dugesia ryukyuensis. / Kobayashi, Kazuya; Maezawa, Takanobu; Tanaka, Hiroyuki; Onuki, Hiroyuki; Horiguchi, Yurie; Hirota, Hiroshi; Ishida, Tetsuo; Horiike, Kihachiro; Agata, Yasutoshi; Aoki, Manabu; Hoshi, Motonori; Matsumoto, Midori.

In: Scientific Reports, Vol. 7, 45175, 24.03.2017.

Research output: Contribution to journalArticle

Kobayashi, K, Maezawa, T, Tanaka, H, Onuki, H, Horiguchi, Y, Hirota, H, Ishida, T, Horiike, K, Agata, Y, Aoki, M, Hoshi, M & Matsumoto, M 2017, 'The identification of D-tryptophan as a bioactive substance for postembryonic ovarian development in the planarian Dugesia ryukyuensis', Scientific Reports, vol. 7, 45175. https://doi.org/10.1038/srep45175
Kobayashi, Kazuya ; Maezawa, Takanobu ; Tanaka, Hiroyuki ; Onuki, Hiroyuki ; Horiguchi, Yurie ; Hirota, Hiroshi ; Ishida, Tetsuo ; Horiike, Kihachiro ; Agata, Yasutoshi ; Aoki, Manabu ; Hoshi, Motonori ; Matsumoto, Midori. / The identification of D-tryptophan as a bioactive substance for postembryonic ovarian development in the planarian Dugesia ryukyuensis. In: Scientific Reports. 2017 ; Vol. 7.
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