The infarcted myocardium solicits GM-CSF for the detrimental oversupply of inflammatory leukocytes

Atsushi Anzai, Jennifer L. Choi, Shun He, Ashley M. Fenn, Manfred Nairz, Sara Rattik, Cameron S. McAlpine, John E. Mindur, Christopher T. Chan, Yoshiko Iwamoto, Benoit Tricot, Gregory R. Wojtkiewicz, Ralph Weissleder, Peter Libby, Matthias Nahrendorf, James R. Stone, Burkhard Becher, Filip K. Swirski

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Myocardial infarction (MI) elicits massive inflammatory leukocyte recruitment to the heart. Here, we hypothesized that excessive leukocyte invasion leads to heart failure and death during acute myocardial ischemia. We found that shortly and transiently after onset of ischemia, human and mouse cardiac fibroblasts produce granulocyte/macrophage colony-stimulating factor (GM-CSF) that acts locally and distally to generate and recruit inflammatory and proteolytic cells. In the heart, fibroblast- derived GM-CSF alerts its neighboring myeloid cells to attract neutrophils and monocytes. The growth factor also reaches the bone marrow, where it stimulates a distinct myeloid-biased progenitor subset. Consequently, hearts of mice deficient in either GM-CSF or its receptor recruit fewer leukocytes and function relatively well, whereas mice producing GM-CSF can succumb from left ventricular rupture, a complication mitigated by anti-GM-CSF therapy. These results identify GM-CSF as both a key contributor to the pathogenesis of MI and a potential therapeutic target, bolstering the idea that GM-CSF is a major orchestrator of the leukocyte supply chain during inflammation.

Original languageEnglish
Pages (from-to)3293-3310
Number of pages18
JournalJournal of Experimental Medicine
Volume214
Issue number11
DOIs
Publication statusPublished - 2017 Nov 1
Externally publishedYes

Fingerprint

Granulocyte-Macrophage Colony-Stimulating Factor
Myocardium
Leukocytes
Fibroblasts
Myocardial Infarction
Myeloid Cells
Myocardial Ischemia
Rupture
Monocytes
Intercellular Signaling Peptides and Proteins
Neutrophils
Ischemia
Heart Failure
Bone Marrow
Inflammation
Therapeutics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

The infarcted myocardium solicits GM-CSF for the detrimental oversupply of inflammatory leukocytes. / Anzai, Atsushi; Choi, Jennifer L.; He, Shun; Fenn, Ashley M.; Nairz, Manfred; Rattik, Sara; McAlpine, Cameron S.; Mindur, John E.; Chan, Christopher T.; Iwamoto, Yoshiko; Tricot, Benoit; Wojtkiewicz, Gregory R.; Weissleder, Ralph; Libby, Peter; Nahrendorf, Matthias; Stone, James R.; Becher, Burkhard; Swirski, Filip K.

In: Journal of Experimental Medicine, Vol. 214, No. 11, 01.11.2017, p. 3293-3310.

Research output: Contribution to journalArticle

Anzai, A, Choi, JL, He, S, Fenn, AM, Nairz, M, Rattik, S, McAlpine, CS, Mindur, JE, Chan, CT, Iwamoto, Y, Tricot, B, Wojtkiewicz, GR, Weissleder, R, Libby, P, Nahrendorf, M, Stone, JR, Becher, B & Swirski, FK 2017, 'The infarcted myocardium solicits GM-CSF for the detrimental oversupply of inflammatory leukocytes', Journal of Experimental Medicine, vol. 214, no. 11, pp. 3293-3310. https://doi.org/10.1084/jem.20170689
Anzai, Atsushi ; Choi, Jennifer L. ; He, Shun ; Fenn, Ashley M. ; Nairz, Manfred ; Rattik, Sara ; McAlpine, Cameron S. ; Mindur, John E. ; Chan, Christopher T. ; Iwamoto, Yoshiko ; Tricot, Benoit ; Wojtkiewicz, Gregory R. ; Weissleder, Ralph ; Libby, Peter ; Nahrendorf, Matthias ; Stone, James R. ; Becher, Burkhard ; Swirski, Filip K. / The infarcted myocardium solicits GM-CSF for the detrimental oversupply of inflammatory leukocytes. In: Journal of Experimental Medicine. 2017 ; Vol. 214, No. 11. pp. 3293-3310.
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