Abstract
Adhesion and spreading of tumor cells to the films of a galactose-, glucose-, or phosphatidylcholine-bearing lipid was studied. Human adenocarcinoma Hela cells, B16 mouse melanoma cells, and HuH-7 human hepatoma cells selectively adhered and spread on galactose-bearing lipid in serum-containing medium, but not in serum-free medium. The spreading of the tumor cells in serum-containing medium was inhibited in the presence of lactose, but not in the presence of maltose. Cell spreading also occured on the galactose-bearing glycolipid film pre-treated with serum. From quantitative analysis for the adsorption of serum components by a quartz-crystal microbalance, the surfaces of the lipid films were found to be entirely covered with serum components. These results suggested that serum components pre-adsorbed on the galactose-bearing lipid influence the cell spreading.
Original language | English |
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Pages (from-to) | 587-599 |
Number of pages | 13 |
Journal | Journal of Biomaterials Science, Polymer Edition |
Volume | 7 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1995 Jan 1 |
Externally published | Yes |
Keywords
- B16 mouse melanoma cell
- Cell spreading
- Fetal bovine serum
- Glycolipid
- Hela cell
- HuH-7 human hepatoma cell
- Phospholipid
- Quartz-crystal microbalance
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering