The influence of serum for spreading of tumor cells on synthetic glycolipid films.

Toshinori Sato, M. Endo, Y. Okahata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Adhesion and spreading of tumor cells to the films of a galactose-, glucose-, or phosphatidylcholine-bearing lipid was studied. Human adenocarcinoma Hela cells, B16 mouse melanoma cells, and HuH-7 human hepatoma cells selectively adhered and spread on galactose-bearing lipid in serum-containing medium, but not in serum-free medium. The spreading of the tumor cells in serum-containing medium was inhibited in the presence of lactose, but not in the presence of maltose. Cell spreading also occured on the galactose-bearing glycolipid film pre-treated with serum. From quantitative analysis for the the adsorption of serum components by a quartz-crystal microbalance, the surfaces of the lipid films were found to be entirely covered with serum components. These results suggested that serum components pre-adsorbed on the galactose-bearing lipid influence the cell spreading.

Original languageEnglish
Pages (from-to)587-599
Number of pages13
JournalJournal of biomaterials science. Polymer edition
Volume7
Issue number7
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Bearings (structural)
Artificial Cells
Glycolipids
Galactose
Lipids
Tumors
Cells
Serum
Neoplasms
Maltose
Quartz crystal microbalances
Serum-Free Culture Media
Lactose
Phosphatidylcholines
Quartz Crystal Microbalance Techniques
Glucose
Experimental Melanomas
Adhesion
HeLa Cells
Adsorption

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials

Cite this

The influence of serum for spreading of tumor cells on synthetic glycolipid films. / Sato, Toshinori; Endo, M.; Okahata, Y.

In: Journal of biomaterials science. Polymer edition, Vol. 7, No. 7, 1995, p. 587-599.

Research output: Contribution to journalArticle

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