Abstract
Adhesion and spreading of tumor cells to the films of a galactose-, glucose-, or phosphatidylcholine-bearing lipid was studied. Human adenocarcinoma Hela cells, B16 mouse melanoma cells, and HuH-7 human hepatoma cells selectively adhered and spread on galactose-bearing lipid in serum-containing medium, but not in serum-free medium. The spreading of the tumor cells in serum-containing medium was inhibited in the presence of lactose, but not in the presence of maltose. Cell spreading also occured on the galactose-bearing glycolipid film pre-treated with serum. From quantitative analysis for the the adsorption of serum components by a quartz-crystal microbalance, the surfaces of the lipid films were found to be entirely covered with serum components. These results suggested that serum components pre-adsorbed on the galactose-bearing lipid influence the cell spreading.
| Original language | English |
|---|---|
| Pages (from-to) | 587-599 |
| Number of pages | 13 |
| Journal | Journal of biomaterials science. Polymer edition |
| Volume | 7 |
| Issue number | 7 |
| Publication status | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
- Biomaterials
Cite this
The influence of serum for spreading of tumor cells on synthetic glycolipid films. / Sato, Toshinori; Endo, M.; Okahata, Y.
In: Journal of biomaterials science. Polymer edition, Vol. 7, No. 7, 1995, p. 587-599.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The influence of serum for spreading of tumor cells on synthetic glycolipid films.
AU - Sato, Toshinori
AU - Endo, M.
AU - Okahata, Y.
PY - 1995
Y1 - 1995
N2 - Adhesion and spreading of tumor cells to the films of a galactose-, glucose-, or phosphatidylcholine-bearing lipid was studied. Human adenocarcinoma Hela cells, B16 mouse melanoma cells, and HuH-7 human hepatoma cells selectively adhered and spread on galactose-bearing lipid in serum-containing medium, but not in serum-free medium. The spreading of the tumor cells in serum-containing medium was inhibited in the presence of lactose, but not in the presence of maltose. Cell spreading also occured on the galactose-bearing glycolipid film pre-treated with serum. From quantitative analysis for the the adsorption of serum components by a quartz-crystal microbalance, the surfaces of the lipid films were found to be entirely covered with serum components. These results suggested that serum components pre-adsorbed on the galactose-bearing lipid influence the cell spreading.
AB - Adhesion and spreading of tumor cells to the films of a galactose-, glucose-, or phosphatidylcholine-bearing lipid was studied. Human adenocarcinoma Hela cells, B16 mouse melanoma cells, and HuH-7 human hepatoma cells selectively adhered and spread on galactose-bearing lipid in serum-containing medium, but not in serum-free medium. The spreading of the tumor cells in serum-containing medium was inhibited in the presence of lactose, but not in the presence of maltose. Cell spreading also occured on the galactose-bearing glycolipid film pre-treated with serum. From quantitative analysis for the the adsorption of serum components by a quartz-crystal microbalance, the surfaces of the lipid films were found to be entirely covered with serum components. These results suggested that serum components pre-adsorbed on the galactose-bearing lipid influence the cell spreading.
UR - http://www.scopus.com/inward/record.url?scp=0029432290&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029432290&partnerID=8YFLogxK
M3 - Article
C2 - 8924425
AN - SCOPUS:0029432290
VL - 7
SP - 587
EP - 599
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 7
ER -