The inhibition of motility of demembranated spermatozoa by anti-tubulin antibodies

Mikako T. Oka, Yoko Nakajima, Masataka Obika, Takao Arai, Yukimaro Nakayama, Yukihisa Hamaguchi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The effects of monoclonal anti-tubulin antibodies on the motility of demembranated and reactivated sea urchin spermatozoa were investigated. Two out of ten antibodies examined significantly reduced the motility of spermatozoa, both in motile rate and swimming speed. The binding patterns of the two antibodies YL1/2 and TUB2.1 to the axoneme were studied by immunoblot, immunofluorescence, and immuno-electron microscopy. YL1/2 bound to the axoneme in a specific pattern; signals were very intense in the tail, rich in the proximal portion, and scarce in the middle part of the axoneme. Because the inhibitory effects of the antibody on the motility of spermatozoa with fully long flagella and short flagella were similar, the inhibition was probably due to the binding of the antibody to the proximal portion of the flagellum. TUB2.1 evenly bound to the axoneme by immunofluorescence and immunoelectron microscopy. On the other hand, the eight antibodies which did not affect sperm motility, did not bind to unfixed axonemes, although epitopes for these antibodies were detected abundantly in the axoneme.

Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalCell structure and function
Volume22
Issue number4
DOIs
Publication statusPublished - 1997 Aug

Keywords

  • Distribution of tubulin isoform
  • Flagella
  • Immunostaining
  • Sea urchin
  • Sperm motility

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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