The KOR-SA3544 antigen predominantly expressed on the surface of Philadelphia chromosome-positive acute lymphoblastic leukemia cells is nonspecific: Cross-reacting antigen-50/90 (CD66c) and invariably expressed in cytoplasm of human leukemia cells

K. Sugita, T. Mori, S. Yokota, Ma Kuroki, T. O-Koyama, T. Inukai, K. Iijima, K. Goi, T. Tezuka, S. Kojika, K. Shiraishi, M. Nakamura, N. Miyamoto, N. Karakida, K. Kagami, S. Nakazawa

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21 Citations (Scopus)

Abstract

We previously reported a novel monoclonal antibody KOR-SA3544 which predominantly reacted with a surface antigen (sSA3544) expressed on Philadelphia chromosome (Ph1)-positive acute lymphoblastic leukemia (ALL). In the present study, we demonstrate that the antibody specifically recognized nonspecific cross-reacting antigen (NCA)-50/90 (CD66c), one of the carcinoembryonic antigen (CEA)-related glycoproteins encoded by a member of the CEA gene family. In addition, we show that the SA3544 antigen (NCA-50/90) was invariably expressed in cytoplasm of all of the human leukemic cell lines examined (sSA3544-positive B-lymphoid two, sSA3544-negative T or B-lymphoid and non-lymphoid 24) regardless of the presence or absence of surface expression of this antigen. Immunoelectromicroscopic examination revealed that the cytoplasmic antigen was mainly present in granules in sSA3544-positive leukemia cells, whereas it was diffusely present in cytosol in sSA3544-negative leukemia cells. Thus, among members of the CEA family, NCA-50/90 was first demonstrated to be expressed not only on the surface of some leukemia cells, but also in cytoplasm of various types of leukemia cells.

Original languageEnglish
Pages (from-to)779-785
Number of pages7
JournalLeukemia
Volume13
Issue number5
DOIs
Publication statusPublished - 1999

Keywords

  • CD66c
  • KOR-SA3544
  • Nonspecific cross-reacting antigen-50/90
  • Philadelphia chromosome-positive acute lymphoblastic leukemia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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