The KOR-SA3544 antigen predominantly expressed on the surface of Philadelphia chromosome-positive acute lymphoblastic leukemia cells is nonspecific: Cross-reacting antigen-50/90 (CD66c) and invariably expressed in cytoplasm of human leukemia cells

K. Sugita, Takehiko Mori, S. Yokota, Ma Kuroki, T. O-Koyama, T. Inukai, K. Iijima, K. Goi, T. Tezuka, S. Kojika, K. Shiraishi, M. Nakamura, N. Miyamoto, N. Karakida, K. Kagami, S. Nakazawa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We previously reported a novel monoclonal antibody KOR-SA3544 which predominantly reacted with a surface antigen (sSA3544) expressed on Philadelphia chromosome (Ph1)-positive acute lymphoblastic leukemia (ALL). In the present study, we demonstrate that the antibody specifically recognized nonspecific cross-reacting antigen (NCA)-50/90 (CD66c), one of the carcinoembryonic antigen (CEA)-related glycoproteins encoded by a member of the CEA gene family. In addition, we show that the SA3544 antigen (NCA-50/90) was invariably expressed in cytoplasm of all of the human leukemic cell lines examined (sSA3544-positive B-lymphoid two, sSA3544-negative T or B-lymphoid and non-lymphoid 24) regardless of the presence or absence of surface expression of this antigen. Immunoelectromicroscopic examination revealed that the cytoplasmic antigen was mainly present in granules in sSA3544-positive leukemia cells, whereas it was diffusely present in cytosol in sSA3544-negative leukemia cells. Thus, among members of the CEA family, NCA-50/90 was first demonstrated to be expressed not only on the surface of some leukemia cells, but also in cytoplasm of various types of leukemia cells.

Original languageEnglish
Pages (from-to)779-785
Number of pages7
JournalLeukemia
Volume13
Issue number5
Publication statusPublished - 1999

Fingerprint

Philadelphia Chromosome
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Cytoplasm
Leukemia
Carcinoembryonic Antigen
Antigens
Surface Antigens
Cytosol
Glycoproteins
Monoclonal Antibodies
Cell Line
Antibodies
Genes

Keywords

  • CD66c
  • KOR-SA3544
  • Nonspecific cross-reacting antigen-50/90
  • Philadelphia chromosome-positive acute lymphoblastic leukemia

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

The KOR-SA3544 antigen predominantly expressed on the surface of Philadelphia chromosome-positive acute lymphoblastic leukemia cells is nonspecific : Cross-reacting antigen-50/90 (CD66c) and invariably expressed in cytoplasm of human leukemia cells. / Sugita, K.; Mori, Takehiko; Yokota, S.; Kuroki, Ma; O-Koyama, T.; Inukai, T.; Iijima, K.; Goi, K.; Tezuka, T.; Kojika, S.; Shiraishi, K.; Nakamura, M.; Miyamoto, N.; Karakida, N.; Kagami, K.; Nakazawa, S.

In: Leukemia, Vol. 13, No. 5, 1999, p. 779-785.

Research output: Contribution to journalArticle

Sugita, K, Mori, T, Yokota, S, Kuroki, M, O-Koyama, T, Inukai, T, Iijima, K, Goi, K, Tezuka, T, Kojika, S, Shiraishi, K, Nakamura, M, Miyamoto, N, Karakida, N, Kagami, K & Nakazawa, S 1999, 'The KOR-SA3544 antigen predominantly expressed on the surface of Philadelphia chromosome-positive acute lymphoblastic leukemia cells is nonspecific: Cross-reacting antigen-50/90 (CD66c) and invariably expressed in cytoplasm of human leukemia cells', Leukemia, vol. 13, no. 5, pp. 779-785.
Sugita, K. ; Mori, Takehiko ; Yokota, S. ; Kuroki, Ma ; O-Koyama, T. ; Inukai, T. ; Iijima, K. ; Goi, K. ; Tezuka, T. ; Kojika, S. ; Shiraishi, K. ; Nakamura, M. ; Miyamoto, N. ; Karakida, N. ; Kagami, K. ; Nakazawa, S. / The KOR-SA3544 antigen predominantly expressed on the surface of Philadelphia chromosome-positive acute lymphoblastic leukemia cells is nonspecific : Cross-reacting antigen-50/90 (CD66c) and invariably expressed in cytoplasm of human leukemia cells. In: Leukemia. 1999 ; Vol. 13, No. 5. pp. 779-785.
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abstract = "We previously reported a novel monoclonal antibody KOR-SA3544 which predominantly reacted with a surface antigen (sSA3544) expressed on Philadelphia chromosome (Ph1)-positive acute lymphoblastic leukemia (ALL). In the present study, we demonstrate that the antibody specifically recognized nonspecific cross-reacting antigen (NCA)-50/90 (CD66c), one of the carcinoembryonic antigen (CEA)-related glycoproteins encoded by a member of the CEA gene family. In addition, we show that the SA3544 antigen (NCA-50/90) was invariably expressed in cytoplasm of all of the human leukemic cell lines examined (sSA3544-positive B-lymphoid two, sSA3544-negative T or B-lymphoid and non-lymphoid 24) regardless of the presence or absence of surface expression of this antigen. Immunoelectromicroscopic examination revealed that the cytoplasmic antigen was mainly present in granules in sSA3544-positive leukemia cells, whereas it was diffusely present in cytosol in sSA3544-negative leukemia cells. Thus, among members of the CEA family, NCA-50/90 was first demonstrated to be expressed not only on the surface of some leukemia cells, but also in cytoplasm of various types of leukemia cells.",
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T1 - The KOR-SA3544 antigen predominantly expressed on the surface of Philadelphia chromosome-positive acute lymphoblastic leukemia cells is nonspecific

T2 - Cross-reacting antigen-50/90 (CD66c) and invariably expressed in cytoplasm of human leukemia cells

AU - Sugita, K.

AU - Mori, Takehiko

AU - Yokota, S.

AU - Kuroki, Ma

AU - O-Koyama, T.

AU - Inukai, T.

AU - Iijima, K.

AU - Goi, K.

AU - Tezuka, T.

AU - Kojika, S.

AU - Shiraishi, K.

AU - Nakamura, M.

AU - Miyamoto, N.

AU - Karakida, N.

AU - Kagami, K.

AU - Nakazawa, S.

PY - 1999

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AB - We previously reported a novel monoclonal antibody KOR-SA3544 which predominantly reacted with a surface antigen (sSA3544) expressed on Philadelphia chromosome (Ph1)-positive acute lymphoblastic leukemia (ALL). In the present study, we demonstrate that the antibody specifically recognized nonspecific cross-reacting antigen (NCA)-50/90 (CD66c), one of the carcinoembryonic antigen (CEA)-related glycoproteins encoded by a member of the CEA gene family. In addition, we show that the SA3544 antigen (NCA-50/90) was invariably expressed in cytoplasm of all of the human leukemic cell lines examined (sSA3544-positive B-lymphoid two, sSA3544-negative T or B-lymphoid and non-lymphoid 24) regardless of the presence or absence of surface expression of this antigen. Immunoelectromicroscopic examination revealed that the cytoplasmic antigen was mainly present in granules in sSA3544-positive leukemia cells, whereas it was diffusely present in cytosol in sSA3544-negative leukemia cells. Thus, among members of the CEA family, NCA-50/90 was first demonstrated to be expressed not only on the surface of some leukemia cells, but also in cytoplasm of various types of leukemia cells.

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