The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons

Tetsuji Mori, Zhang Yuxing, Hiromi Takaki, Mayumi Takeuchi, Ken Iseki, Seita Hagino, Jun Ichi Kitanaka, Motohiko Takemura, Hidemi Misawa, Masahito Ikawa, Masaru Okabe, Akio Wanaka

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Basal forebrain cholinergic neurons (BFCNs) are involved in cognitive functions such as learning and memory, and are affected in several neurodegenerative diseases (e.g. Alzheimer's disease). Despite their importance, the molecular mechanisms of their development are not fully elucidated. A recent report demonstrated that some BFCNs in adult rat are positive for L3/Lhx8, a LIM homeobox transcription factor. To examine the function of L3/Lhx8 in the development of BFCNs, we generated L3/Lhx8 gene-disrupted mice. In these mice, cells expressing cholinergic neuron markers, such as choline acetyltransferase, vesicular acetylcholine transporter and p75 low-affinity NGF receptor, were markedly reduced in the basal forebrain, whereas other cholinergic neurons including brain stem and spinal motor neurons expressed the markers. Neurotransmitter phenotypes other than cholinergic in the basal forebrain appeared intact. From these results, we suggested that L3/Lhx8 has a pivotal and specific role in the development and/or maintenance of BFCNs.

Original languageEnglish
Pages (from-to)3129-3141
Number of pages13
JournalEuropean Journal of Neuroscience
Volume19
Issue number12
DOIs
Publication statusPublished - 2004 Jun
Externally publishedYes

Fingerprint

Cholinergic Neurons
Homeobox Genes
Vesicular Acetylcholine Transport Proteins
Nerve Growth Factor Receptor
Choline O-Acetyltransferase
Motor Neurons
Neurodegenerative Diseases
Cognition
Cholinergic Agents
Brain Stem
Neurotransmitter Agents
Basal Forebrain
Alzheimer Disease
Transcription Factors
Maintenance
Learning
Phenotype
Genes

Keywords

  • Brain
  • Choline acetyltransferase
  • LIM domain
  • Mouse
  • Null mutant
  • Transcription factor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons. / Mori, Tetsuji; Yuxing, Zhang; Takaki, Hiromi; Takeuchi, Mayumi; Iseki, Ken; Hagino, Seita; Kitanaka, Jun Ichi; Takemura, Motohiko; Misawa, Hidemi; Ikawa, Masahito; Okabe, Masaru; Wanaka, Akio.

In: European Journal of Neuroscience, Vol. 19, No. 12, 06.2004, p. 3129-3141.

Research output: Contribution to journalArticle

Mori, T, Yuxing, Z, Takaki, H, Takeuchi, M, Iseki, K, Hagino, S, Kitanaka, JI, Takemura, M, Misawa, H, Ikawa, M, Okabe, M & Wanaka, A 2004, 'The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons', European Journal of Neuroscience, vol. 19, no. 12, pp. 3129-3141. https://doi.org/10.1111/j.0953-816X.2004.03415.x
Mori, Tetsuji ; Yuxing, Zhang ; Takaki, Hiromi ; Takeuchi, Mayumi ; Iseki, Ken ; Hagino, Seita ; Kitanaka, Jun Ichi ; Takemura, Motohiko ; Misawa, Hidemi ; Ikawa, Masahito ; Okabe, Masaru ; Wanaka, Akio. / The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons. In: European Journal of Neuroscience. 2004 ; Vol. 19, No. 12. pp. 3129-3141.
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