The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza

Masayuki Morita, Keiji Kuba, Akihiko Ichikawa, Mizuho Nakayama, Jun Katahira, Ryo Iwamoto, Tokiko Watanebe, Saori Sakabe, Tomo Daidoji, Shota Nakamura, Ayumi Kadowaki, Takayo Ohto, Hiroki Nakanishi, Ryo Taguchi, Takaaki Nakaya, Makoto Murakami, Yoshihiro Yoneda, Hiroyuki Arai, Yoshihiro Kawaoka, Josef M. PenningerMakoto Arita, Yumiko Imai

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Influenza A viruses are a major cause of mortality. Given the potential for future lethal pandemics, effective drugs are needed for the treatment of severe influenza such as that caused by H5N1 viruses. Using mediator lipidomics and bioactive lipid screen, we report that the omega-3 polyunsaturated fatty acid (PUFA)-derived lipid mediator protectin D1 (PD1) markedly attenuated influenza virus replication via RNA export machinery. Production of PD1 was suppressed during severe influenza and PD1 levels inversely correlated with the pathogenicity of H5N1 viruses. Suppression of PD1 was genetically mapped to 12/15-lipoxygenase activity. Importantly, PD1 treatment improved the survival and pathology of severe influenza in mice, even under conditions where known antiviral drugs fail to protect from death. These results identify the endogenous lipid mediator PD1 as an innate suppressor of influenza virus replication that protects against lethal influenza virus infection.

Original languageEnglish
Pages (from-to)112-125
Number of pages14
JournalCell
Volume153
Issue number1
DOIs
Publication statusPublished - 2013 Mar 28
Externally publishedYes

Fingerprint

Virus Replication
Orthomyxoviridae
Viruses
Human Influenza
Lipids
H5N1 Subtype Influenza A Virus
Influenza A virus
Omega-3 Fatty Acids
Pandemics
Pathology
Virus Diseases
Unsaturated Fatty Acids
Machinery
Antiviral Agents
Virulence
protectin D1
RNA
Mortality
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Morita, M., Kuba, K., Ichikawa, A., Nakayama, M., Katahira, J., Iwamoto, R., ... Imai, Y. (2013). The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza. Cell, 153(1), 112-125. https://doi.org/10.1016/j.cell.2013.02.027

The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza. / Morita, Masayuki; Kuba, Keiji; Ichikawa, Akihiko; Nakayama, Mizuho; Katahira, Jun; Iwamoto, Ryo; Watanebe, Tokiko; Sakabe, Saori; Daidoji, Tomo; Nakamura, Shota; Kadowaki, Ayumi; Ohto, Takayo; Nakanishi, Hiroki; Taguchi, Ryo; Nakaya, Takaaki; Murakami, Makoto; Yoneda, Yoshihiro; Arai, Hiroyuki; Kawaoka, Yoshihiro; Penninger, Josef M.; Arita, Makoto; Imai, Yumiko.

In: Cell, Vol. 153, No. 1, 28.03.2013, p. 112-125.

Research output: Contribution to journalArticle

Morita, M, Kuba, K, Ichikawa, A, Nakayama, M, Katahira, J, Iwamoto, R, Watanebe, T, Sakabe, S, Daidoji, T, Nakamura, S, Kadowaki, A, Ohto, T, Nakanishi, H, Taguchi, R, Nakaya, T, Murakami, M, Yoneda, Y, Arai, H, Kawaoka, Y, Penninger, JM, Arita, M & Imai, Y 2013, 'The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza', Cell, vol. 153, no. 1, pp. 112-125. https://doi.org/10.1016/j.cell.2013.02.027
Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R et al. The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza. Cell. 2013 Mar 28;153(1):112-125. https://doi.org/10.1016/j.cell.2013.02.027
Morita, Masayuki ; Kuba, Keiji ; Ichikawa, Akihiko ; Nakayama, Mizuho ; Katahira, Jun ; Iwamoto, Ryo ; Watanebe, Tokiko ; Sakabe, Saori ; Daidoji, Tomo ; Nakamura, Shota ; Kadowaki, Ayumi ; Ohto, Takayo ; Nakanishi, Hiroki ; Taguchi, Ryo ; Nakaya, Takaaki ; Murakami, Makoto ; Yoneda, Yoshihiro ; Arai, Hiroyuki ; Kawaoka, Yoshihiro ; Penninger, Josef M. ; Arita, Makoto ; Imai, Yumiko. / The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza. In: Cell. 2013 ; Vol. 153, No. 1. pp. 112-125.
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