The lung as a possible target organ for atrial natriuretic polypeptide secreted from the heart

Makoto Sakamoto, Kazuwa Nakao, Narito Morii, Akira Sugawara, Takayuki Yamada, Hiroshi Itoh, Shozo Shiono, Yoshihiko Saito, Hiroo Imura

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Using a specific radioimmunoassay (RIA) for α-rat atrial natriuretic polypeptide (α-rANP), we have demonstrated the presence of a considerable amount (6.10 ± 0.38 ng/g) (mean ± SE) of α-rANP-like immunoreactivity (α-rANP-LI) in the rat lung, the first organ through which atrial natriuretic polypeptide (ANP) released from the heart passes. High performance gel permeation chromatography coupled with the RIA revealed that most of α-rANP-LI eluted at the position of a low molecular weight form corresponding to synthetic α-rANP. In 2- or 5-day water-deprived rats, the concentration and content of α-rANP-LI in the lung decreased significantly compared with those of control rats. In addition, water-deprivation induced a significant decrease in the plasma concentration of α-rANP-LI simultaneously determined. There was a significant positive correlation between the concentrations of α-rANP-LI in the lung and plasma (r=0.591, P<0.01). These results indicate the presence of ANP in the lung and suggest physiological roles of ANP in pulmonary function.

Original languageEnglish
Pages (from-to)515-520
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume135
Issue number2
DOIs
Publication statusPublished - 1986 Mar 13
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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