TY - JOUR
T1 - The lung as a possible target organ for atrial natriuretic polypeptide secreted from the heart
AU - Sakamoto, Makoto
AU - Nakao, Kazuwa
AU - Morii, Narito
AU - Sugawara, Akira
AU - Yamada, Takayuki
AU - Itoh, Hiroshi
AU - Shiono, Shozo
AU - Saito, Yoshihiko
AU - Imura, Hiroo
N1 - Funding Information:
We thank Miss H. Fumon and K. Horii for the secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1985, and Yamanouchi Foundation for Research on
Funding Information:
Metabolic Disorders, by a research (60A-3) from the Japanese Ministry Heart Foundation Research Grant for
PY - 1986/3/13
Y1 - 1986/3/13
N2 - Using a specific radioimmunoassay (RIA) for α-rat atrial natriuretic polypeptide (α-rANP), we have demonstrated the presence of a considerable amount (6.10 ± 0.38 ng/g) (mean ± SE) of α-rANP-like immunoreactivity (α-rANP-LI) in the rat lung, the first organ through which atrial natriuretic polypeptide (ANP) released from the heart passes. High performance gel permeation chromatography coupled with the RIA revealed that most of α-rANP-LI eluted at the position of a low molecular weight form corresponding to synthetic α-rANP. In 2- or 5-day water-deprived rats, the concentration and content of α-rANP-LI in the lung decreased significantly compared with those of control rats. In addition, water-deprivation induced a significant decrease in the plasma concentration of α-rANP-LI simultaneously determined. There was a significant positive correlation between the concentrations of α-rANP-LI in the lung and plasma (r=0.591, P<0.01). These results indicate the presence of ANP in the lung and suggest physiological roles of ANP in pulmonary function.
AB - Using a specific radioimmunoassay (RIA) for α-rat atrial natriuretic polypeptide (α-rANP), we have demonstrated the presence of a considerable amount (6.10 ± 0.38 ng/g) (mean ± SE) of α-rANP-like immunoreactivity (α-rANP-LI) in the rat lung, the first organ through which atrial natriuretic polypeptide (ANP) released from the heart passes. High performance gel permeation chromatography coupled with the RIA revealed that most of α-rANP-LI eluted at the position of a low molecular weight form corresponding to synthetic α-rANP. In 2- or 5-day water-deprived rats, the concentration and content of α-rANP-LI in the lung decreased significantly compared with those of control rats. In addition, water-deprivation induced a significant decrease in the plasma concentration of α-rANP-LI simultaneously determined. There was a significant positive correlation between the concentrations of α-rANP-LI in the lung and plasma (r=0.591, P<0.01). These results indicate the presence of ANP in the lung and suggest physiological roles of ANP in pulmonary function.
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U2 - 10.1016/0006-291X(86)90024-0
DO - 10.1016/0006-291X(86)90024-0
M3 - Article
C2 - 2938578
AN - SCOPUS:0022452711
SN - 0006-291X
VL - 135
SP - 515
EP - 520
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -