The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein

Yamato Kikkawa, Takaho Ogawa, Ryo Sudo, Yuji Yamada, Fumihiko Katagiri, Kentaro Hozumi, Motoyoshi Nomizu, Jeffrey H. Miner

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cell-matrix interactions are critical for tumor cell migration. Lutheran (Lu), also known as basal cell adhesion molecule (B-CAM), competes with integrins for binding to laminin α5, a subunit of LM-511, a major component of basement membranes. Here we show that the preferential binding of Lu/B-CAM to laminin α5 promotes tumor cell migration. The attachment of Lu/B-CAM transfectants to LM-511 was slightly weaker than that of control cells, and this was because Lu/B-CAM disturbed integrin binding to laminin α5. Lu/B-CAM induced a spindle cell shape with pseudopods and promoted cell migration on LM-511. In addition, blocking with an anti-Lu/B-CAM antibody led to a flat cell shape and inhibited migration on LM-511, similar to the effects of an activating integrin β1 antibody. We conclude that tumor cell migration on LM-511 requires that Lu/B-CAM competitively modulates cell attachment through integrins. We suggest that this competitive interaction is involved in a balance between static and migratory cell behaviors.

Original languageEnglish
Pages (from-to)30990-31001
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number43
DOIs
Publication statusPublished - 2013 Oct 25

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Cell Adhesion Molecules
Integrins
Cell Movement
Tumors
Cells
Neoplasms
Proteins
Cell Shape
Pseudopodia
Antibodies
laminin 10
Basement Membrane
Cell Communication
kalinin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein. / Kikkawa, Yamato; Ogawa, Takaho; Sudo, Ryo; Yamada, Yuji; Katagiri, Fumihiko; Hozumi, Kentaro; Nomizu, Motoyoshi; Miner, Jeffrey H.

In: Journal of Biological Chemistry, Vol. 288, No. 43, 25.10.2013, p. 30990-31001.

Research output: Contribution to journalArticle

Kikkawa, Yamato ; Ogawa, Takaho ; Sudo, Ryo ; Yamada, Yuji ; Katagiri, Fumihiko ; Hozumi, Kentaro ; Nomizu, Motoyoshi ; Miner, Jeffrey H. / The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 43. pp. 30990-31001.
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AU - Katagiri, Fumihiko

AU - Hozumi, Kentaro

AU - Nomizu, Motoyoshi

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