The majority of bullous pemphigoid and herpes gestationis serum samples react with the NC16a domain of the 180-kDa bullous pemphigoid antigen

K. Matsumura, Masayuki Amagai, T. Nishikawa, T. Hashimoto

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

A series of previous studies have indicated that the NC16a domain of the 180-kDa bullous pemphigoid antigen (BP180) is the most immunogenic and probably pathogenic region in both bullous pemphigoid and herpes gestationis. To confirm these previous results by a large-scale study, we examined serum from 154 bullous pemphigoid and 43 herpes gestationis patients using an immunoblot technique with the BP180 NC16a domain fusion protein, which had been prepared using cDNA obtained from a human keratinocyte cDNA library by polymerase chain reaction amplification. This fusion protein was recognized by 90% of bullous pemphigoid and 79% of herpes gestationis serum samples, but not by any other disease or normal control serum samples. These results indicate that this system is very sensitive and specific for the detection of anti-BP180 antibodies and should be useful for the diagnosis of various subepidermal bullous diseases.

Original languageEnglish
Pages (from-to)507-509
Number of pages3
JournalArchives of Dermatological Research
Volume288
Issue number9
DOIs
Publication statusPublished - 1996 Aug

Fingerprint

Pemphigoid Gestationis
Bullous Pemphigoid
Antigens
Serum
Gene Library
Keratinocytes
Anti-Idiotypic Antibodies
Complementary DNA
Polymerase Chain Reaction
Proteins

Keywords

  • Autoantigen
  • Basement membrane zone
  • Bullous pemphigoid
  • Immunoblot
  • Recombinant protein

ASJC Scopus subject areas

  • Dermatology

Cite this

The majority of bullous pemphigoid and herpes gestationis serum samples react with the NC16a domain of the 180-kDa bullous pemphigoid antigen. / Matsumura, K.; Amagai, Masayuki; Nishikawa, T.; Hashimoto, T.

In: Archives of Dermatological Research, Vol. 288, No. 9, 08.1996, p. 507-509.

Research output: Contribution to journalArticle

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