The measurement of the phosphorescence and singlet oxygen fluorescence time-resolved waveforms of Photofrin(II)® and Talaporfin Sodium with pulsed excitation

Shiho Hakomori, Sayaka Ohmori, Kensuke Masuda, Kojiro Yamamoto, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In order to investigate the interaction between the triplet state T 1 and ground state oxygen 3O2 during pulsed excitation photodynamic therapy (PDT), we measured the phosphorescence and singlet oxygen 1O2 fluorescence timeresolved waveform. The phosphorescence time-resolved waveform from clinical photosensitizers has not been obtained because this signal was buried in the photosensitizer fluorescence. We constructed the experimental setup with a spectral and temporal filter to select the phosphorescence signals from the Photofrin(II)® and Talaporfin sodium solution. The lifetimes and spectrums of the measured luminescence coincided with the phosphorescence characteristics, respectively. We obtained the phosphorescence time-resolved waveforms from the clinical photosensitizer solutions successfully. The 1O2 fluorescence time-resolved waveforms from these photosensitizers were measured with an IR-PMT with a photon counter. The fluorescence time-resolved waveforms of each photosensitizer were also obtained by the authors. We could consequently describe sequential generation of three time-resolved waveforms throughout the photosensitive reaction in the clinical photosensitizers. We think we may evaluate the photoseisitizer characteristics by these waveforms.

Original languageEnglish
Title of host publicationOptical Methods for Tumor Treatment and Detection
Subtitle of host publicationMechanisms and Techniques in Photodynamic Therapy XV
DOIs
Publication statusPublished - 2007 Aug 31
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI - San Jose, CA, United States
Duration: 2007 Jan 202007 Jan 21

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6427
ISSN (Print)1605-7422

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI
CountryUnited States
CitySan Jose, CA
Period07/1/2007/1/21

Keywords

  • Phosphorescence
  • Photodynamic therapy
  • Photofrin(II)®
  • Pulsed excitation
  • Singlet oxygen
  • Talaporfin sodium
  • Time-resolved waveform

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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