The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT

Kouji Hase, Takaya Murakami, Hiroyuki Takatsu, Takeshi Shimaoka, Mitsutoshi Iimura, Kimiyo Hamura, Kazuya Kawano, Sayaka Ohshima, Risa Chihara, Kikuji Itoh, Shin Yonehara, Hiroshi Ohno

Research output: Contribution to journalArticle

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Abstract

The recently identified CXCL16 has dual functions as a transmembrane adhesion molecule and a soluble chemokine. In this study we found that CXCL16 mRNA and protein were expressed constitutively on the follicle-associated epithelium covering Peyer's patches (PPs), isolated lymphoid follicles, and cecal patches, but minimally on the villous epithelium in the murine gastrointestinal tract. The CXCL16 receptor CXCR6/Bonzo was constitutively expressed on subpopulations of CD4 + and CD8 + T cells isolated from PPs. The expression of CXCR6/Bonzo on the PP T cells was up-regulated after stimulation with anti-CD3 and anti-CD28 mAbs. The activated PP T cells showed chemotactic migration in response to the soluble N-terminal chemokine domain of CXCL16. Furthermore, the activated PP T cells selectively adhered to cells expressing murine CXCL16. To determine the physiological role of CXCL16 in GALT, we first carefully analyzed T cell distribution in PPs. T cells localized not only in the interfollicular region but also at a lesser frequency in the subepithelial dome (SED) and in the germinal center of lymphoid follicles. Consistently, the majority of the adoptive transferred activated T cells migrated into the SED and the interfollicular region. However, the neutralization of CXCL16 specifically reduced the migration of the adoptive, transferred, activated T cells into the SED of PPs. These data suggest that CXCL16 expressed on the follicle-associated epithelium plays an important role in the recruitment and retention of activated T cells in the SED and should, at least partially, be responsible for lymphocyte compartmentalization in GALT.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalJournal of Immunology
Volume176
Issue number1
Publication statusPublished - 2006 Jan 1
Externally publishedYes

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Chemokines
Peyer's Patches
Epithelium
T-Lymphocytes
Membranes
Germinal Center
Gastrointestinal Tract
Lymphocytes
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT. / Hase, Kouji; Murakami, Takaya; Takatsu, Hiroyuki; Shimaoka, Takeshi; Iimura, Mitsutoshi; Hamura, Kimiyo; Kawano, Kazuya; Ohshima, Sayaka; Chihara, Risa; Itoh, Kikuji; Yonehara, Shin; Ohno, Hiroshi.

In: Journal of Immunology, Vol. 176, No. 1, 01.01.2006, p. 43-51.

Research output: Contribution to journalArticle

Hase, K, Murakami, T, Takatsu, H, Shimaoka, T, Iimura, M, Hamura, K, Kawano, K, Ohshima, S, Chihara, R, Itoh, K, Yonehara, S & Ohno, H 2006, 'The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT', Journal of Immunology, vol. 176, no. 1, pp. 43-51.
Hase, Kouji ; Murakami, Takaya ; Takatsu, Hiroyuki ; Shimaoka, Takeshi ; Iimura, Mitsutoshi ; Hamura, Kimiyo ; Kawano, Kazuya ; Ohshima, Sayaka ; Chihara, Risa ; Itoh, Kikuji ; Yonehara, Shin ; Ohno, Hiroshi. / The membrane-bound chemokine CXCL16 expressed on follicle-associated epithelium and M cells mediates lympho-epithelial interaction in GALT. In: Journal of Immunology. 2006 ; Vol. 176, No. 1. pp. 43-51.
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