The mouse Sox5 gene encodes a protein containing the leucine zipper and the Q box

Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Susumu Kido; Sadakazu Aiso

doi:10.1016/S0167-4781(98)00086-4

The mouse Sox5 gene encodes a protein containing the leucine zipper and the Q box

Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Susumu Kido, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The nucleotide sequence of mouse Sox5 (mSox5) cDNA derived from the testis has been reported by Denny et al. (EMBO J. 11 (1992) 3705-3712). We newly isolated an mSox5 cDNA derived from 8.5-day mouse embryo. Our cDNA encodes a protein of 763 amino acids, which is considerably larger in size than the previous one (392 amino acids) derived from the adult mouse testis. The most significant difference between the embryo-derived and testis- derived mSox5 cDNAs is that the embryonic one encodes a leucine zipper motif and a neighboring glutamine-rich sequence stretch (named Q box), but the testis-derived one does not. The leucine zipper and the Q box are highly conserved among type-D SOX proteins including mSox5. mSox5 was suggested to be a single-copy gene by Southern analysis. With reverse transcription/polymerase chain reaction, we found that not only mouse embryo, but also the adult mouse testis, express mSox5 mRNA species encoding the leucine zipper and the Q box.

Original language	English
Pages (from-to)	40-46
Number of pages	7
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1399
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(98)00086-4
Publication status	Published - 1998 Jul 30
Externally published	Yes

Keywords

Embryo
HMG box
Leucine zipper
Q box
SRY
Testis

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(98)00086-4

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title = "The mouse Sox5 gene encodes a protein containing the leucine zipper and the Q box",

abstract = "The nucleotide sequence of mouse Sox5 (mSox5) cDNA derived from the testis has been reported by Denny et al. (EMBO J. 11 (1992) 3705-3712). We newly isolated an mSox5 cDNA derived from 8.5-day mouse embryo. Our cDNA encodes a protein of 763 amino acids, which is considerably larger in size than the previous one (392 amino acids) derived from the adult mouse testis. The most significant difference between the embryo-derived and testis- derived mSox5 cDNAs is that the embryonic one encodes a leucine zipper motif and a neighboring glutamine-rich sequence stretch (named Q box), but the testis-derived one does not. The leucine zipper and the Q box are highly conserved among type-D SOX proteins including mSox5. mSox5 was suggested to be a single-copy gene by Southern analysis. With reverse transcription/polymerase chain reaction, we found that not only mouse embryo, but also the adult mouse testis, express mSox5 mRNA species encoding the leucine zipper and the Q box.",

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author = "Yoshiki Hiraoka and Motoyuki Ogawa and Yukinao Sakai and Susumu Kido and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A. and Grants-in Aids from Ministry of Education, Science and Culture to S.A. and Y.H., and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.",

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AU - Kido, Susumu

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A. and Grants-in Aids from Ministry of Education, Science and Culture to S.A. and Y.H., and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.

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N2 - The nucleotide sequence of mouse Sox5 (mSox5) cDNA derived from the testis has been reported by Denny et al. (EMBO J. 11 (1992) 3705-3712). We newly isolated an mSox5 cDNA derived from 8.5-day mouse embryo. Our cDNA encodes a protein of 763 amino acids, which is considerably larger in size than the previous one (392 amino acids) derived from the adult mouse testis. The most significant difference between the embryo-derived and testis- derived mSox5 cDNAs is that the embryonic one encodes a leucine zipper motif and a neighboring glutamine-rich sequence stretch (named Q box), but the testis-derived one does not. The leucine zipper and the Q box are highly conserved among type-D SOX proteins including mSox5. mSox5 was suggested to be a single-copy gene by Southern analysis. With reverse transcription/polymerase chain reaction, we found that not only mouse embryo, but also the adult mouse testis, express mSox5 mRNA species encoding the leucine zipper and the Q box.

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The mouse Sox5 gene encodes a protein containing the leucine zipper and the Q box

Abstract

Keywords

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