The myocilin (MYOC) gene expression in the human trabecular meshwork

Hiroki Takahashi, Setsuko Noda, Yukihiko Mashima, Ryo Kubota, Yuichiro Ohtake, Tomihiko Tanino, Jun Kudo, Shinsei Minoshima, Yoshihisa Oguchi, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Purpose. We previously reported a novel cytoskeletal protein with a myosin-like domain which is localized in the ciliary rootlet and basal body of connecting cilium of photoreceptor and hence we named it 'myocilin'. It was soon realized that myocilin is identical to a protein called TIGR (trabecular meshwork inducible glucocorticoid response protein) which was found to be responsible for the pathogenesis of juvenile open angle glaucoma. In this study, we employed in situ RNA hybridization to examine the myocilin (MYOC)/TIGR gene expression in the trabecular meshworks of glaucomatous and nonglaucomatous eyes. Methods. The glaucomatous specimens were obtained by trabeculectomy from the patients with primary open angle glaucoma (POAG), chronic angle closure glaucoma (CACG) and steroid glaucoma, respectively, and the nonglaucomatous specimens were obtained from a victim of traffic accident at autopsy and from a patient with maxillary sinus carcinoma at enucleation for the operation. The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. Results. In all cases, hybridization signals were detected primarily in the trabecular meshwork cells and secondarily in the fibroblast-like cells of corneoscleral wall. Conclusions. Myocilin gene is expressed clearly in the trabecular meshwork cells of both glaucomatous and nonglaucomatous eyes.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalCurrent Eye Research
Volume20
Issue number2
Publication statusPublished - 2000 Feb

Fingerprint

Trabecular Meshwork
Gene Expression
Photoreceptor Connecting Cilium
Glucocorticoids
In Situ Hybridization
RNA
Basal Bodies
RNA Probes
Angle Closure Glaucoma
Digoxigenin
Antisense RNA
Ciliary Body
Proteins
Trabeculectomy
Cytoskeletal Proteins
Maxillary Sinus
Traffic Accidents
Open Angle Glaucoma
Myosins
Glaucoma

Keywords

  • GLC1A
  • Myocilin
  • POAG (primary open angle glaucoma)
  • TIGR (trabecular meshwork inducible glucocorticoid response protein)
  • Trabecular meshwork

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Takahashi, H., Noda, S., Mashima, Y., Kubota, R., Ohtake, Y., Tanino, T., ... Shimizu, N. (2000). The myocilin (MYOC) gene expression in the human trabecular meshwork. Current Eye Research, 20(2), 81-84.

The myocilin (MYOC) gene expression in the human trabecular meshwork. / Takahashi, Hiroki; Noda, Setsuko; Mashima, Yukihiko; Kubota, Ryo; Ohtake, Yuichiro; Tanino, Tomihiko; Kudo, Jun; Minoshima, Shinsei; Oguchi, Yoshihisa; Shimizu, Nobuyoshi.

In: Current Eye Research, Vol. 20, No. 2, 02.2000, p. 81-84.

Research output: Contribution to journalArticle

Takahashi, H, Noda, S, Mashima, Y, Kubota, R, Ohtake, Y, Tanino, T, Kudo, J, Minoshima, S, Oguchi, Y & Shimizu, N 2000, 'The myocilin (MYOC) gene expression in the human trabecular meshwork', Current Eye Research, vol. 20, no. 2, pp. 81-84.
Takahashi H, Noda S, Mashima Y, Kubota R, Ohtake Y, Tanino T et al. The myocilin (MYOC) gene expression in the human trabecular meshwork. Current Eye Research. 2000 Feb;20(2):81-84.
Takahashi, Hiroki ; Noda, Setsuko ; Mashima, Yukihiko ; Kubota, Ryo ; Ohtake, Yuichiro ; Tanino, Tomihiko ; Kudo, Jun ; Minoshima, Shinsei ; Oguchi, Yoshihisa ; Shimizu, Nobuyoshi. / The myocilin (MYOC) gene expression in the human trabecular meshwork. In: Current Eye Research. 2000 ; Vol. 20, No. 2. pp. 81-84.
@article{919d15b7aeb14112ae26740143428aa9,
title = "The myocilin (MYOC) gene expression in the human trabecular meshwork",
abstract = "Purpose. We previously reported a novel cytoskeletal protein with a myosin-like domain which is localized in the ciliary rootlet and basal body of connecting cilium of photoreceptor and hence we named it 'myocilin'. It was soon realized that myocilin is identical to a protein called TIGR (trabecular meshwork inducible glucocorticoid response protein) which was found to be responsible for the pathogenesis of juvenile open angle glaucoma. In this study, we employed in situ RNA hybridization to examine the myocilin (MYOC)/TIGR gene expression in the trabecular meshworks of glaucomatous and nonglaucomatous eyes. Methods. The glaucomatous specimens were obtained by trabeculectomy from the patients with primary open angle glaucoma (POAG), chronic angle closure glaucoma (CACG) and steroid glaucoma, respectively, and the nonglaucomatous specimens were obtained from a victim of traffic accident at autopsy and from a patient with maxillary sinus carcinoma at enucleation for the operation. The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. Results. In all cases, hybridization signals were detected primarily in the trabecular meshwork cells and secondarily in the fibroblast-like cells of corneoscleral wall. Conclusions. Myocilin gene is expressed clearly in the trabecular meshwork cells of both glaucomatous and nonglaucomatous eyes.",
keywords = "GLC1A, Myocilin, POAG (primary open angle glaucoma), TIGR (trabecular meshwork inducible glucocorticoid response protein), Trabecular meshwork",
author = "Hiroki Takahashi and Setsuko Noda and Yukihiko Mashima and Ryo Kubota and Yuichiro Ohtake and Tomihiko Tanino and Jun Kudo and Shinsei Minoshima and Yoshihisa Oguchi and Nobuyoshi Shimizu",
year = "2000",
month = "2",
language = "English",
volume = "20",
pages = "81--84",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - The myocilin (MYOC) gene expression in the human trabecular meshwork

AU - Takahashi, Hiroki

AU - Noda, Setsuko

AU - Mashima, Yukihiko

AU - Kubota, Ryo

AU - Ohtake, Yuichiro

AU - Tanino, Tomihiko

AU - Kudo, Jun

AU - Minoshima, Shinsei

AU - Oguchi, Yoshihisa

AU - Shimizu, Nobuyoshi

PY - 2000/2

Y1 - 2000/2

N2 - Purpose. We previously reported a novel cytoskeletal protein with a myosin-like domain which is localized in the ciliary rootlet and basal body of connecting cilium of photoreceptor and hence we named it 'myocilin'. It was soon realized that myocilin is identical to a protein called TIGR (trabecular meshwork inducible glucocorticoid response protein) which was found to be responsible for the pathogenesis of juvenile open angle glaucoma. In this study, we employed in situ RNA hybridization to examine the myocilin (MYOC)/TIGR gene expression in the trabecular meshworks of glaucomatous and nonglaucomatous eyes. Methods. The glaucomatous specimens were obtained by trabeculectomy from the patients with primary open angle glaucoma (POAG), chronic angle closure glaucoma (CACG) and steroid glaucoma, respectively, and the nonglaucomatous specimens were obtained from a victim of traffic accident at autopsy and from a patient with maxillary sinus carcinoma at enucleation for the operation. The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. Results. In all cases, hybridization signals were detected primarily in the trabecular meshwork cells and secondarily in the fibroblast-like cells of corneoscleral wall. Conclusions. Myocilin gene is expressed clearly in the trabecular meshwork cells of both glaucomatous and nonglaucomatous eyes.

AB - Purpose. We previously reported a novel cytoskeletal protein with a myosin-like domain which is localized in the ciliary rootlet and basal body of connecting cilium of photoreceptor and hence we named it 'myocilin'. It was soon realized that myocilin is identical to a protein called TIGR (trabecular meshwork inducible glucocorticoid response protein) which was found to be responsible for the pathogenesis of juvenile open angle glaucoma. In this study, we employed in situ RNA hybridization to examine the myocilin (MYOC)/TIGR gene expression in the trabecular meshworks of glaucomatous and nonglaucomatous eyes. Methods. The glaucomatous specimens were obtained by trabeculectomy from the patients with primary open angle glaucoma (POAG), chronic angle closure glaucoma (CACG) and steroid glaucoma, respectively, and the nonglaucomatous specimens were obtained from a victim of traffic accident at autopsy and from a patient with maxillary sinus carcinoma at enucleation for the operation. The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. Results. In all cases, hybridization signals were detected primarily in the trabecular meshwork cells and secondarily in the fibroblast-like cells of corneoscleral wall. Conclusions. Myocilin gene is expressed clearly in the trabecular meshwork cells of both glaucomatous and nonglaucomatous eyes.

KW - GLC1A

KW - Myocilin

KW - POAG (primary open angle glaucoma)

KW - TIGR (trabecular meshwork inducible glucocorticoid response protein)

KW - Trabecular meshwork

UR - http://www.scopus.com/inward/record.url?scp=6744231317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6744231317&partnerID=8YFLogxK

M3 - Article

C2 - 10617907

AN - SCOPUS:6744231317

VL - 20

SP - 81

EP - 84

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 2

ER -