The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation

Christoph S.N. Klose, Tanel Mahlakõiv, Jesper B. Moeller, Lucille C. Rankin, Anne Laure Flamar, Hiroki Kabata, Laurel A. Monticelli, Saya Moriyama, Gregory Garbès Putzel, Nikolai Rakhilin, Xiling Shen, Evi Kostenis, Gabriele M. König, Takashi Senda, Dustin Carpenter, Donna L. Farber, David Artis

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

The type 2 cytokines interleukin (IL)-4, IL-5, IL-9 and IL-13 have important roles in stimulating innate and adaptive immune responses that are required for resistance to helminth infection, promotion of allergic inflammation, metabolic homeostasis and tissue repair. Group 2 innate lymphoid cells (ILC2s) produce type 2 cytokines, and although advances have been made in understanding the cytokine milieu that promotes ILC2 responses, how ILC2 responses are regulated by other stimuli remains poorly understood. Here we demonstrate that ILC2s in the mouse gastrointestinal tract co-localize with cholinergic neurons that express the neuropeptide neuromedin U (NMU). In contrast to other haematopoietic cells, ILC2s selectively express the NMU receptor 1 (NMUR1). In vitro stimulation of ILC2s with NMU induced rapid cell activation, proliferation, and secretion of the type 2 cytokines IL-5, IL-9 and IL-13 that was dependent on cell-intrinsic expression of NMUR1 and G αq protein. In vivo administration of NMU triggered potent type 2 cytokine responses characterized by ILC2 activation, proliferation and eosinophil recruitment that was associated with accelerated expulsion of the gastrointestinal nematode Nippostrongylus brasiliensis or induction of lung inflammation. Conversely, worm burden was higher in Nmur1 â '/â ' mice than in control mice. Furthermore, use of gene-deficient mice and adoptive cell transfer experiments revealed that ILC2s were necessary and sufficient to mount NMU-elicited type 2 cytokine responses. Together, these data indicate that the NMU-NMUR1 neuronal signalling circuit provides a selective mechanism through which the enteric nervous system and innate immune system integrate to promote rapid type 2 cytokine responses that can induce anti-microbial, inflammatory and tissue-protective type 2 responses at mucosal sites.

Original languageEnglish
Pages (from-to)282-286
Number of pages5
JournalNature
Volume549
Issue number7671
DOIs
Publication statusPublished - 2017 Sep 14
Externally publishedYes

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Neuropeptides
Lymphocytes
Cytokines
Inflammation
Interleukin-9
Interleukin-13
Interleukin-5
Nippostrongylus
Enteric Nervous System
Cholinergic Neurons
Adoptive Transfer
Helminths
Adaptive Immunity
neuromedin U
Eosinophils
Innate Immunity
Interleukin-4
Gastrointestinal Tract
Immune System
Pneumonia

ASJC Scopus subject areas

  • General

Cite this

Klose, C. S. N., Mahlakõiv, T., Moeller, J. B., Rankin, L. C., Flamar, A. L., Kabata, H., ... Artis, D. (2017). The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation. Nature, 549(7671), 282-286. https://doi.org/10.1038/nature23676

The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation. / Klose, Christoph S.N.; Mahlakõiv, Tanel; Moeller, Jesper B.; Rankin, Lucille C.; Flamar, Anne Laure; Kabata, Hiroki; Monticelli, Laurel A.; Moriyama, Saya; Putzel, Gregory Garbès; Rakhilin, Nikolai; Shen, Xiling; Kostenis, Evi; König, Gabriele M.; Senda, Takashi; Carpenter, Dustin; Farber, Donna L.; Artis, David.

In: Nature, Vol. 549, No. 7671, 14.09.2017, p. 282-286.

Research output: Contribution to journalArticle

Klose, CSN, Mahlakõiv, T, Moeller, JB, Rankin, LC, Flamar, AL, Kabata, H, Monticelli, LA, Moriyama, S, Putzel, GG, Rakhilin, N, Shen, X, Kostenis, E, König, GM, Senda, T, Carpenter, D, Farber, DL & Artis, D 2017, 'The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation', Nature, vol. 549, no. 7671, pp. 282-286. https://doi.org/10.1038/nature23676
Klose CSN, Mahlakõiv T, Moeller JB, Rankin LC, Flamar AL, Kabata H et al. The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation. Nature. 2017 Sep 14;549(7671):282-286. https://doi.org/10.1038/nature23676
Klose, Christoph S.N. ; Mahlakõiv, Tanel ; Moeller, Jesper B. ; Rankin, Lucille C. ; Flamar, Anne Laure ; Kabata, Hiroki ; Monticelli, Laurel A. ; Moriyama, Saya ; Putzel, Gregory Garbès ; Rakhilin, Nikolai ; Shen, Xiling ; Kostenis, Evi ; König, Gabriele M. ; Senda, Takashi ; Carpenter, Dustin ; Farber, Donna L. ; Artis, David. / The neuropeptide neuromedin U stimulates innate lymphoid cells and type 2 inflammation. In: Nature. 2017 ; Vol. 549, No. 7671. pp. 282-286.
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